Abstract
We isolated large quantities of mesophyll protoplasts from source and sink leaves of soybean plants and examined them for amino acid uptake. Accumulation of amino acids in isolated protoplasts was linear for at least 40 minutes. Uptake kinetics revealed the presence of both saturable and linear components. Increasing external pH decreases the uptake. The uncoupler, carbonyl cyanide p-trifluoromethoxyphenylhydrazone at 15 micromolar inhibited and fusicoccin at 10 micromolar stimulated amino acid uptake. Our data are consistent with a proton-cotransport mechanism for the uptake of l-glutamine and alpha-amino isobutyric acid into soybean mesophyll cells.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
Amino acid accumulation was linear for at least 40 minutes.
- Taxon
- Soybean
Glycine maxt0 - Outcome
- Accumulation of amino acidse0
- Qualifier
- for at least 40 minutese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of amino acids in isolated protoplasts was linear for at least 40 minutes.”
t0“Soybean”
Claim · model-written summary
Uptake kinetics had saturable and linear components.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- Uptake kineticse0
- Qualifier
- both saturable and linear componentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Uptake kinetics revealed the presence of both saturable and linear components.”
t0“Soybean”
Claim · model-written summary
Increasing external pH decreased amino acid uptake.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- external pHe0
- Outcome
- uptakee0
- Direction
- decreasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing external pH decreases the uptake.”
t0“Soybean”
Claim · model-written summary
Carbonyl cyanide p-trifluoromethoxyphenylhydrazone inhibited amino acid uptake.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- carbonyl cyanide p-trifluoromethoxyphenylhydrazone at 15 micromolare0
- Outcome
- amino acid uptakee0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The uncoupler, carbonyl cyanide p-trifluoromethoxyphenylhydrazone at 15 micromolar inhibited and fusicoccin at 10 micromolar stimulated amino acid uptake.”
t0“Soybean”
Claim · model-written summary
Fusicoccin stimulated amino acid uptake.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- fusicoccin at 10 micromolare0
- Outcome
- amino acid uptakee0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The uncoupler, carbonyl cyanide p-trifluoromethoxyphenylhydrazone at 15 micromolar inhibited and fusicoccin at 10 micromolar stimulated amino acid uptake.”
t0“Soybean”
Claim · model-written summary
Authors interpret l-glutamine and alpha-amino isobutyric acid uptake as proton cotransport.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- mesophyll cellse0
- Outcome
- uptake of l-glutamine and alpha-amino isobutyric acide0
- Qualifier
- proton-cotransport mechanisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our data are consistent with a proton-cotransport mechanism for the uptake of l-glutamine and alpha-amino isobutyric acid into soybean mesophyll cells.”
The same questions in other papers
carbonyl cyanide p-trifluoromethoxyphenylhydrazone · nutrient: content · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- negative
- negative
fusicoccin · nutrient: content · Glycine max (soybean)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.