Abstract
The plasma membrane H+-ATPase plays an important role in the plant response to nutrient and environmental stresses. However, the involvement of plant root plasma membrane H+-ATPase in adaptation to phosphate (P) starvation is not yet fully elucidated. In this study, experiments were performed with soybean roots in low-P nutrient solution (10 microM). Treatment with fusicoccin, an activator of the plasma membrane H+-ATPase, increased P uptake by 35%, while vanadate, an inhibitor of plasma membrane H+-ATPase, severely suppressed it. These results suggested that P uptake might be regulated via the modulation of the activity of plasma membrane H+-ATPase under P starvation. The relationship between P uptake and the activity of plasma membrane H+-ATPase was examined further by using plasma membrane H+-ATPase transgenic Arabidopsis thaliana under low-P conditions. Transgenic plants absorbed more P compared with wild-type Arabidopsis. Results from real-time RT-PCR, western-blotting and immunolocalization analysis indicated that the increase in activity of the plasma membrane H+-ATPase by P starvation was caused by its transcriptional and translational regulation. A higher expression was observed at the translational level than at the transcriptional level. P starvation could induce a transient increase of endogenous indole-3-acetic acid (IAA) in soybean roots. The exogenous application of IAA stimulated the activity of plasma membrane H+-ATPase and P uptake, while naphthylphthalamic acid (NPA), an IAA transport inhibitor, blocked IAA effects. Taken together, these results suggested an involvement of root plasma membrane H+-ATPase in the adaptation of soybean to P starvation. IAA might be involved in signal transduction of P starvation by activating the plasma membrane H+-ATPase in soybean roots.
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Claims 10
Claim · model-written summary
Fusicoccin, an activator of plasma membrane H+-ATPase, increased phosphorus uptake by 35% in soybean roots.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adaptation of soybean to phosphorus starvation”
e1“Treatment with fusicoccin, an activator of the plasma membrane H+-ATPase, increased P uptake by 35%”
Claim · model-written summary
Vanadate, an inhibitor of plasma membrane H+-ATPase, severely suppressed phosphorus uptake in soybean roots.
- Taxon
- soybean
Glycine maxt0 - Treatment
- vanadatee2
- Outcome
- P uptakee1
- Direction
- suppressede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adaptation of soybean to phosphorus starvation”
e1“Treatment with fusicoccin, an activator of the plasma membrane H+-ATPase, increased P uptake by 35%”
e2“while vanadate, an inhibitor of plasma membrane H+-ATPase, severely suppressed it”
Claim · model-written summary
Transgenic Arabidopsis thaliana with higher plasma membrane H+-ATPase absorbed more P than wild-type Arabidopsis under low-P conditions.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae4 - Treatment
- plasma membrane H+-ATPase transgenice4
- Outcome
- Pe3
- Direction
- moree3
- Comparator
- wild-type Arabidopsise3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Transgenic plants absorbed more P compared with wild-type Arabidopsis”
e4“plasma membrane H+-ATPase transgenic Arabidopsis thaliana under low-P conditions”
Claim · model-written summary
Phosphorus starvation increased plasma membrane H+-ATPase activity through transcriptional and translational regulation in soybean.
- Taxon
- soybeanGlycine maxt0
- Treatment
- P starvatione5
- Outcome
- activity of the plasma membrane H+-ATPasee5
- Qualifier
- transcriptional and translational regulatione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adaptation of soybean to phosphorus starvation”
e5“the increase in activity of the plasma membrane H+-ATPase by P starvation was caused by its transcriptional and translational regulation”
Claim · model-written summary
Expression of plasma membrane H+-ATPase was higher at the translational level than at the transcriptional level under phosphorus starvation.
- Taxon
- soybeanGlycine maxt0
- Outcome
- expressione6
- Direction
- highere6
- Qualifier
- at the translational level than at the transcriptional levele6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adaptation of soybean to phosphorus starvation”
e6“A higher expression was observed at the translational level than at the transcriptional level”
Claim · model-written summary
Phosphorus starvation induced a transient increase of endogenous indole-3-acetic acid in soybean roots.
- Taxon
- soybeanGlycine maxt0
- Treatment
- P starvatione7
- Outcome
- endogenous indole-3-acetic acide7
- Direction
- increasee7
- Qualifier
- transiente7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adaptation of soybean to phosphorus starvation”
e7“P starvation could induce a transient increase of endogenous indole-3-acetic acid (IAA) in soybean roots”
Claim · model-written summary
Exogenous IAA stimulated plasma membrane H+-ATPase activity and phosphorus uptake in soybean.
- Taxon
- soybeanGlycine maxt0
- Treatment
- exogenous application of IAAe7
- Outcome
- the activity of plasma membrane H+-ATPase and P uptakee7
- Direction
- stimulatede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adaptation of soybean to phosphorus starvation”
e7“The exogenous application of IAA stimulated the activity of plasma membrane H+-ATPase and P uptake”
Claim · model-written summary
Naphthylphthalamic acid, an IAA transport inhibitor, blocked IAA effects in soybean.
- Taxon
- soybeanGlycine maxt0
- Treatment
- naphthylphthalamic acid (NPA)e7
- Outcome
- IAA effectse7
- Direction
- blockede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adaptation of soybean to phosphorus starvation”
e7“naphthylphthalamic acid (NPA), an IAA transport inhibitor, blocked IAA effects”
Claim · model-written summary
IAA may be involved in phosphorus starvation signal transduction by activating plasma membrane H+-ATPase in soybean roots.
- Taxon
- soybeanGlycine maxt0
- Outcome
- signal transduction of P starvatione7
- Qualifier
- by activating the plasma membrane H+-ATPasee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adaptation of soybean to phosphorus starvation”
e7“IAA might be involved in signal transduction of P starvation by activating the plasma membrane H+-ATPase in soybean roots”
Claim · model-written summary
Root plasma membrane H+-ATPase is suggested to be involved in the adaptation of soybean to phosphorus starvation.
- Taxon
- soybeanGlycine maxt0
- Outcome
- adaptation of soybean to P starvatione7
- Qualifier
- root plasma membrane H+-ATPasee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“adaptation of soybean to phosphorus starvation”
e7“Taken together, these results suggested an involvement of root plasma membrane H+-ATPase in the adaptation of soybean to P starvation”
The same questions in other papers
indole-3-acetic acid · nutrient: content · Glycine max (soybean)Positive and negative results
6 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
4 papers more in this group
See all 8 papers →fusicoccin · nutrient: content · Glycine max (soybean)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.