Abstract
Previous studies with isolated soybean cotyledon protoplasts revealed the presence of a saturable, simple diffusion, and nonsaturating carrier-mediated uptake of sucrose into soybean cotyledon cells. A proton/sucrose cotransport may be involved in the saturable sucrose uptake (Lin et al. 1984 Plant Physiol 75: 936-940 and Schmitt et al. 1984 Plant Physiol 75: 941-946). In this study, we investigated the linear sucrose uptake mechanism by treating isolated protoplasts with 15 micromolar p-trifluoromethoxy-carbonylcyanide phenylhydrazone (FCCP) or 100 micromolar p-chloromecuribenzenesulfonic acid to eliminate the saturable uptake. We found: (a) increasing external pH decreases the linear sucrose uptake; (b) fusicoccin at 20 micromolar stimulates and FCCP at 15 micromolar inhibits this linear sucrose uptake; and (c) the ratio of the initial influx of proton to sucrose is close to one in both saturable and nondiffusive linear (difference between the total linear and diffusive components) uptakes. The results suggest that a proton/sucrose cotransport is also involved in the nondiffusive linear sucrose uptake into soybean cotyledon cells.
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Claims 6
Claim · model-written summary
Higher external pH decreased linear sucrose uptake.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- increasing external pHe0
- Outcome
- linear sucrose uptakee0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increasing external pH decreases the linear sucrose uptake”
t0“Soybean Cotyledons”
Claim · model-written summary
Fusicoccin at 20 micromolar stimulated linear sucrose uptake.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- fusicoccin at 20 micromolare0
- Outcome
- linear sucrose uptakee0
- Direction
- stimulatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fusicoccin at 20 micromolar stimulates and FCCP at 15 micromolar inhibits this linear sucrose uptake”
t0“Soybean Cotyledons”
Claim · model-written summary
FCCP at 15 micromolar inhibited linear sucrose uptake.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- FCCP at 15 micromolare0
- Outcome
- linear sucrose uptakee0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fusicoccin at 20 micromolar stimulates and FCCP at 15 micromolar inhibits this linear sucrose uptake”
t0“Soybean Cotyledons”
Claim · model-written summary
Initial proton-to-sucrose influx ratio was near one in saturable uptake.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- ratio of the initial influx of proton to sucrosee0
- Value
- close to onee0
- Qualifier
- saturablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ratio of the initial influx of proton to sucrose is close to one in both saturable and nondiffusive linear (difference between the total linear and diffusive components) uptakes”
t0“Soybean Cotyledons”
Claim · model-written summary
Initial proton-to-sucrose influx ratio was near one in nondiffusive linear uptake.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- ratio of the initial influx of proton to sucrosee0
- Value
- close to onee0
- Qualifier
- nondiffusive lineare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the ratio of the initial influx of proton to sucrose is close to one in both saturable and nondiffusive linear (difference between the total linear and diffusive components) uptakes”
t0“Soybean Cotyledons”
Claim · model-written summary
Authors infer proton/sucrose cotransport contributes to nondiffusive linear uptake.
- Taxon
- soybeanGlycine maxe0
- Outcome
- nondiffusive linear sucrose uptakee0
- Qualifier
- proton/sucrose cotransporte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results suggest that a proton/sucrose cotransport is also involved in the nondiffusive linear sucrose uptake into soybean cotyledon cells”
The same questions in other papers
carbonyl cyanide p-trifluoromethoxyphenylhydrazone · nutrient: content · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- negative
- negative
fusicoccin · nutrient: content · Glycine max (soybean)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.