Paper · 1971 · American Journal of Botany

THE CULTURE OF APICAL BUDS OF XANTHIUM AND THEIR USE AS A BIOASSAY FOR FLOWERING ACTIVITY OF ECDYSTERONE

William P. Jacobs; Hannah B. Suthers

5
claims
10
verbatim quotes
28
citations
2
research areas
—
Not open access

Abstract

Conditions were developed for the sterile culture of shoot tips of Xanthium pensylvanicum Wallr. for use as a bioassay for flower‐controlling chemicals. By using a modified Murashige‐Skoog medium (minus the auxin but including kinetin) and light intensity much higher than usual for plant tissue cultures, fast growth and development of the shoot tips was achieved. Under short‐day conditions (8 hr day: 16 hr night), the cultures from vegetative shoots flowered and fruited; under noninductive conditions (using a 2 hr light‐break in the middle of the dark period), the shoot tips continued vegetative development. Both intact plants and cultured tips could be photoinduced in the first days after germination. Ecdysterone, a potent insect moulting hormone, was tested in the bioassay system. It was without either qualitative or quantitative effect on flowering or vegetative development on either cultured shoot tips or intact plants irrespective of whether they were under inductive or noninductive photoperiodic conditions.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Modified medium and high light produced fast shoot-tip growth and development.

Taxon
Xanthium pensylvanicumXanthium strumariume1
Treatment
modified Murashige‐Skoog medium (minus the auxin but including kinetin) and light intensity much higher than usuale0
Outcome
fast growth and development of the shoot tipse0
Direction
faste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Xanthium strumarium
Xanthium strumarium · TaxonGeorge Chernilevsky · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“By using a modified Murashige‐Skoog medium (minus the auxin but including kinetin) and light intensity much higher than usual for plant tissue cultures, fast growth and development of the shoot tips was achieved”

✓ located verbatim in the source · abstract, characters 153–364 · show in text ↑
e1

“shoot tips of Xanthium pensylvanicum Wallr.”

✓ located verbatim in the source · abstract, characters 53–96 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Vegetative shoot cultures flowered and fruited under short days.

Taxon
Xanthium pensylvanicumXanthium strumariume1
Treatment
short‐day conditions (8 hr day: 16 hr night)e0
Outcome
flowered and fruitede0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Xanthium strumarium
Xanthium strumarium · TaxonGeorge Chernilevsky · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Under short‐day conditions (8 hr day: 16 hr night), the cultures from vegetative shoots flowered and fruited”

✓ located verbatim in the source · abstract, characters 366–474 · show in text ↑
e1

“shoot tips of Xanthium pensylvanicum Wallr.”

✓ located verbatim in the source · abstract, characters 53–96 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Shoot tips remained vegetative with a light break in the dark period.

Taxon
Xanthium pensylvanicumXanthium strumariume1
Treatment
2 hr light‐break in the middle of the dark periode0
Outcome
continued vegetative developmente0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Xanthium strumarium
Xanthium strumarium · TaxonGeorge Chernilevsky · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“under noninductive conditions (using a 2 hr light‐break in the middle of the dark period), the shoot tips continued vegetative development”

✓ located verbatim in the source · abstract, characters 476–614 · show in text ↑
e1

“shoot tips of Xanthium pensylvanicum Wallr.”

✓ located verbatim in the source · abstract, characters 53–96 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Both intact plants and cultured tips were photoinducible soon after germination.

Taxon
Xanthium pensylvanicumXanthium strumariume1
Organ / stage
first days after germinatione0
Outcome
photoinducede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Xanthium strumarium
Xanthium strumarium · TaxonGeorge Chernilevsky · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Both intact plants and cultured tips could be photoinduced in the first days after germination”

✓ located verbatim in the source · abstract, characters 616–710 · show in text ↑
e1

“shoot tips of Xanthium pensylvanicum Wallr.”

✓ located verbatim in the source · abstract, characters 53–96 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Ecdysterone had no qualitative or quantitative effect on flowering or vegetative development.

Taxon
Xanthium pensylvanicumXanthium strumariume1
Treatment
Ecdysteronee0
Outcome
flowering or vegetative developmente0
Direction
without either qualitative or quantitative effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Xanthium strumarium
Xanthium strumarium · TaxonGeorge Chernilevsky · CC BY 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Ecdysterone, a potent insect moulting hormone, was tested in the bioassay system. It was without either qualitative or quantitative effect on flowering or vegetative development on either cultured shoot tips or intact plants irrespective of whether they were under inductive or noninductive photoperiodic conditions”

✓ located verbatim in the source · abstract, characters 712–1027 · show in text ↑
e1

“shoot tips of Xanthium pensylvanicum Wallr.”

✓ located verbatim in the source · abstract, characters 53–96 · show in text ↑

light · growth · Xanthium strumarium (cocklebur)Positive and negative results

7 papers: 4 positive, 3 negative or no effect

3 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

class · growth · Xanthium strumarium (cocklebur)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.