Abstract
Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in mediating biotic and abiotic stress responses. Cotton (Gossypium hirsutum) is the most important textile crop in the world, and often encounters abiotic stress during its growth seasons. In this study, a gene encoding a mitogen-activated protein kinase (MAPK) was isolated from cotton, and designated as GhMPK17. The open reading frame (ORF) of GhMPK17 gene is 1494 bp in length and encodes a protein with 497 amino acids. Quantitative RT-PCR analysis indicated that GhMPK17 expression was up-regulated in cotton under NaCl, mannitol and ABA treatments. The transgenic Arabidopsis plants expressing GhMPK17 gene showed higher seed germination, root elongation and cotyledon greening/expansion rates than those of the wild type on MS medium containing NaCl, mannitol and exogenous ABA, suggesting that overexpression of GhMPK17 in Arabidopsis increased plant ABA-insensitivity, and enhanced plant tolerance to salt and osmotic stresses. Furthermore, overexpression of GhMPK17 in Arabidopsis reduced H2O2 level and altered expression of ABA- and abiotic stress-related genes in the transgenic plants. Collectively, these data suggested that GhMPK17 gene may be involved in plant response to high salinity and osmotic stresses and ABA signaling.
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Claims 12
Claim · model-written summary
A cotton MAPK gene was isolated and named GhMPK17.
- Taxon
- cotton
Gossypium hirsutume0 - Gene
- GhMPK17e0
- Outcome
- gene encoding a mitogen-activated protein kinase (MAPK)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a gene encoding a mitogen-activated protein kinase (MAPK) was isolated from cotton, and designated as GhMPK17”
Claim · model-written summary
GhMPK17 has a 1494 bp open reading frame and encodes a protein of 497 amino acids.
- Taxon
- cottonGossypium hirsutume1
- Gene
- GhMPK17e0
- Outcome
- open reading frame (ORF)e0
- Value
- 1494 bpe0
- Qualifier
- 497 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The open reading frame (ORF) of GhMPK17 gene is 1494 bp in length and encodes a protein with 497 amino acids”
e1“isolated from cotton”
Claim · model-written summary
NaCl treatment up-regulated GhMPK17 expression in cotton.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- NaCle0
- Gene
- GhMPK17e0
- Outcome
- GhMPK17 expressione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhMPK17 expression was up-regulated in cotton under NaCl, mannitol and ABA treatments”
Claim · model-written summary
Mannitol treatment up-regulated GhMPK17 expression in cotton.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- mannitole0
- Gene
- GhMPK17e0
- Outcome
- GhMPK17 expressione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhMPK17 expression was up-regulated in cotton under NaCl, mannitol and ABA treatments”
Claim · model-written summary
ABA treatment up-regulated GhMPK17 expression in cotton.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- ABAe0
- Gene
- GhMPK17e0
- Outcome
- GhMPK17 expressione0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhMPK17 expression was up-regulated in cotton under NaCl, mannitol and ABA treatments”
Claim · model-written summary
Transgenic Arabidopsis expressing GhMPK17 had higher seed germination, root elongation and cotyledon greening or expansion rates than wild type on NaCl medium.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- NaCle0
- Gene
- GhMPK17e0
- Outcome
- seed germination, root elongation and cotyledon greening/expansion ratese0
- Direction
- highere0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic Arabidopsis plants expressing GhMPK17 gene showed higher seed germination, root elongation and cotyledon greening/expansion rates than those of the wild type on MS medium containing NaCl, mannitol and exogenous ABA”
Claim · model-written summary
Transgenic Arabidopsis expressing GhMPK17 had higher seed germination, root elongation and cotyledon greening or expansion rates than wild type on mannitol medium.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- mannitole0
- Gene
- GhMPK17e0
- Outcome
- seed germination, root elongation and cotyledon greening/expansion ratese0
- Direction
- highere0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic Arabidopsis plants expressing GhMPK17 gene showed higher seed germination, root elongation and cotyledon greening/expansion rates than those of the wild type on MS medium containing NaCl, mannitol and exogenous ABA”
Claim · model-written summary
Transgenic Arabidopsis expressing GhMPK17 had higher seed germination, root elongation and cotyledon greening or expansion rates than wild type on exogenous ABA medium.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- exogenous ABAe0
- Gene
- GhMPK17e0
- Outcome
- seed germination, root elongation and cotyledon greening/expansion ratese0
- Direction
- highere0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transgenic Arabidopsis plants expressing GhMPK17 gene showed higher seed germination, root elongation and cotyledon greening/expansion rates than those of the wild type on MS medium containing NaCl, mannitol and exogenous ABA”
Claim · model-written summary
GhMPK17 overexpression reduced H2O2 level in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpression of GhMPK17e0
- Gene
- GhMPK17e0
- Outcome
- H2O2 levele0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GhMPK17 in Arabidopsis reduced H2O2 level and altered expression of ABA- and abiotic stress-related genes in the transgenic plants”
Claim · model-written summary
GhMPK17 overexpression altered ABA- and abiotic stress-related gene expression in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpression of GhMPK17e0
- Gene
- GhMPK17e0
- Outcome
- expression of ABA- and abiotic stress-related genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GhMPK17 in Arabidopsis reduced H2O2 level and altered expression of ABA- and abiotic stress-related genes in the transgenic plants”
Claim · model-written summary
The authors inferred that GhMPK17 overexpression increases ABA insensitivity in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpression of GhMPK17e0
- Gene
- GhMPK17e0
- Outcome
- plant ABA-insensitivitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GhMPK17 in Arabidopsis increased plant ABA-insensitivity”
Claim · model-written summary
The authors inferred that GhMPK17 overexpression enhances salt and osmotic stress tolerance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpression of GhMPK17e0
- Gene
- GhMPK17e0
- Outcome
- plant tolerance to salt and osmotic stressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GhMPK17 in Arabidopsis increased plant ABA-insensitivity, and enhanced plant tolerance to salt and osmotic stresses”
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