Abstract
The transcription factors C-repeat binding factors/dehydration-responsive element binding proteins (CBFs/DREBs) control the expression of many stress-inducible genes in Arabidopsis. A cDNA clone, designated GhDREB1, was isolated from cotton (Gossypium hirsutum) by cDNA library screening. Northern blot analysis indicated that mRNA accumulation of GhDREB1 was induced by low temperatures and salt stress, but was not induced by abscisic acid (ABA) or drought stress in cotton seedlings. Transgenic tobacco (Nicotiana tabacum) plants overexpressing GhDREB1 displayed stronger chilling tolerance than wild-type plants. Their leaf chlorophyll fluorescence, net photosynthetic rate and proline concentrations were higher than those of control plants during low-temperature treatment. However, under normal growth conditions, the transgenic tobacco plants exhibited retarded growth and delayed flowering. Interestingly, GhDREB1 transcripts in cotton seedlings were negatively regulated by gibberellic acid (GA(3)) treatment. Analysis of the promoter of the GhDREB1 gene revealed the presence of one low-temperature and four gibberellin-responsive elements. Green fluorescent protein (GFP) signal intensity or beta-glucuronidase (GUS) activity driven by the GhDREB1 promoter was clearly enhanced by low temperature but repressed by GA(3). These results suggest that GhDREB1 functions as a transcription factor and plays an important role in improving cold tolerance, and also affects plant growth and development via GA(3).
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Claims 11
Claim · model-written summary
Low temperature induced GhDREB1 mRNA in cotton seedlings.
- Taxon
- cotton
Gossypium hirsutume0 - Treatment
- low temperaturese0
- Gene
- GhDREB1e0
- Outcome
- mRNA accumulatione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mRNA accumulation of GhDREB1 was induced by low temperatures and salt stress, but was not induced by abscisic acid (ABA) or drought stress in cotton seedlings.”
Claim · model-written summary
Salt stress induced GhDREB1 mRNA in cotton seedlings.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- salt stresse0
- Gene
- GhDREB1e0
- Outcome
- mRNA accumulatione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mRNA accumulation of GhDREB1 was induced by low temperatures and salt stress, but was not induced by abscisic acid (ABA) or drought stress in cotton seedlings.”
Claim · model-written summary
ABA did not induce GhDREB1 mRNA in cotton seedlings.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- abscisic acid (ABA)e0
- Gene
- GhDREB1e0
- Outcome
- mRNA accumulatione0
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mRNA accumulation of GhDREB1 was induced by low temperatures and salt stress, but was not induced by abscisic acid (ABA) or drought stress in cotton seedlings.”
Claim · model-written summary
Drought did not induce GhDREB1 mRNA in cotton seedlings.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- drought stresse0
- Gene
- GhDREB1e0
- Outcome
- mRNA accumulatione0
- Direction
- not inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mRNA accumulation of GhDREB1 was induced by low temperatures and salt stress, but was not induced by abscisic acid (ABA) or drought stress in cotton seedlings.”
Claim · model-written summary
GhDREB1 tobacco had greater chilling tolerance than wild type.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- overexpressing GhDREB1e0
- Gene
- GhDREB1e0
- Outcome
- chilling tolerancee0
- Direction
- strongere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic tobacco (Nicotiana tabacum) plants overexpressing GhDREB1 displayed stronger chilling tolerance than wild-type plants.”
Claim · model-written summary
During cold treatment, GhDREB1 tobacco had higher chlorophyll fluorescence, photosynthetic rate and proline than controls.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- overexpressing GhDREB1e1
- Outcome
- leaf chlorophyll fluorescence, net photosynthetic rate and proline concentrationse0
- Direction
- highere0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Their leaf chlorophyll fluorescence, net photosynthetic rate and proline concentrations were higher than those of control plants during low-temperature treatment.”
e1“Transgenic tobacco (Nicotiana tabacum) plants overexpressing GhDREB1”
Claim · model-written summary
Under normal conditions, GhDREB1 tobacco had retarded growth and delayed flowering.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- retarded growth and delayed floweringe0
- Qualifier
- normal growth conditionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under normal growth conditions, the transgenic tobacco plants exhibited retarded growth and delayed flowering.”
Claim · model-written summary
GA3 negatively regulated GhDREB1 transcripts in cotton seedlings.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- gibberellic acid (GA(3)) treatmente0
- Gene
- GhDREB1e0
- Outcome
- GhDREB1 transcriptse0
- Direction
- negatively regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhDREB1 transcripts in cotton seedlings were negatively regulated by gibberellic acid (GA(3)) treatment.”
Claim · model-written summary
GhDREB1 promoter contains one low-temperature and four gibberellin-responsive elements.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- GhDREB1e0
- Outcome
- presence of one low-temperature and four gibberellin-responsive elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the GhDREB1 gene revealed the presence of one low-temperature and four gibberellin-responsive elements”
t0“Cotton”
Claim · model-written summary
Low temperature enhanced GhDREB1 promoter driven reporter signals.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- low temperaturee0
- Gene
- GhDREB1e0
- Outcome
- Green fluorescent protein (GFP) signal intensity or beta-glucuronidase (GUS) activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Green fluorescent protein (GFP) signal intensity or beta-glucuronidase (GUS) activity driven by the GhDREB1 promoter was clearly enhanced by low temperature but repressed by GA(3).”
t0“Cotton”
Claim · model-written summary
GA3 repressed GhDREB1 promoter driven reporter signals.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- GA(3)e0
- Gene
- GhDREB1e0
- Outcome
- Green fluorescent protein (GFP) signal intensity or beta-glucuronidase (GUS) activitye0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Green fluorescent protein (GFP) signal intensity or beta-glucuronidase (GUS) activity driven by the GhDREB1 promoter was clearly enhanced by low temperature but repressed by GA(3).”
t0“Cotton”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
3 papers in the same direction as this paper · 12 in the opposite direction
- positive
- positive
- positive
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See all 16 papers →drought · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
10 papers in the same direction as this paper · 19 in the opposite direction
- positive
- positive
- positive
26 papers more in this group
See all 30 papers →overexpression · physiology: photosynthesis · Nicotiana tabacum (tobacco)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.