Abstract
The ethylene-responsive element-binding factor(ERF) gene family is one of the largest transcription factor gene families in plants.In this study,a novel ERF gene named GhERF8(GenBank: JN656957) was isolated from our laboratory's leaf cDNA library for Gossypium hirsutum L.The gene encodes a putative of 265 amino-acid protein with a conserved AP2 domain.To understand the function of GhERF8,real-time quantitative PCR(RT-PCR) was used to evaluate the relative expression of GhERF8 at different developmental stages of leaves and tissues.The results showed that GhERF8 could express in all tissues,but expression levels differed.The expression of GhERF8 was the highest in the later stage of leaf maturation.During leaf senescence,GhERF8 expression and chlorophyll content were inversely correlated,indicating that GhERF8 could be related to leaf senescence.Under ethephon and jasmonic acid(JA) treatment,the expression of GhERF8 was up-regulated in leaves,but there was no significant change in expression of GhERF8 under abscisic acid(ABA) treatment.These results indicated that GhERF8 could participate in the signal transduction of ethylene and JA,but not in the ABA-dependent pathway.
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Claims 11
Claim · model-written summary
A novel GhERF8 gene was isolated from a Gossypium hirsutum leaf cDNA library.
- Taxon
- Gossypium hirsutum L.
Gossypium hirsutume0 - Gene
- GhERF8e0
- Outcome
- was isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a novel ERF gene named GhERF8(GenBank: JN656957) was isolated from our laboratory's leaf cDNA library for Gossypium hirsutum L.”
Claim · model-written summary
GhERF8 encodes a putative 265 amino-acid protein with a conserved AP2 domain.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Gene
- GhERF8t0
- Outcome
- protein with a conserved AP2 domaine0
- Value
- 265 amino-acide0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The gene encodes a putative of 265 amino-acid protein with a conserved AP2 domain.”
t0“GhERF8 Gene in Gossypium hirsutum L.”
Claim · model-written summary
GhERF8 was expressed in all tissues, with differing levels.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Gene
- GhERF8e0
- Outcome
- express in all tissuese0
- Qualifier
- expression levels differede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhERF8 could express in all tissues,but expression levels differed.”
t0“Gossypium hirsutum L.”
Claim · model-written summary
GhERF8 expression was highest in the later stage of leaf maturation.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Organ / stage
- later stage of leaf maturatione0
- Gene
- GhERF8e0
- Outcome
- expressione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of GhERF8 was the highest in the later stage of leaf maturation.”
t0“Gossypium hirsutum L.”
Claim · model-written summary
During leaf senescence, GhERF8 expression and chlorophyll content were inversely correlated.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Organ / stage
- leaf senescencee0
- Gene
- GhERF8e0
- Outcome
- chlorophyll contente0
- Direction
- inversely correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During leaf senescence,GhERF8 expression and chlorophyll content were inversely correlated”
t0“Gossypium hirsutum L.”
Claim · model-written summary
GhERF8 may be related to leaf senescence.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Gene
- GhERF8e0
- Outcome
- leaf senescencee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicating that GhERF8 could be related to leaf senescence.”
t0“Gossypium hirsutum L.”
Claim · model-written summary
Ethephon treatment up-regulated GhERF8 expression in leaves.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Organ / stage
- leavese0
- Treatment
- ethephone0
- Gene
- GhERF8e0
- Outcome
- expression of GhERF8e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under ethephon and jasmonic acid(JA) treatment,the expression of GhERF8 was up-regulated in leaves”
t0“Gossypium hirsutum L.”
Claim · model-written summary
Jasmonic acid treatment up-regulated GhERF8 expression in leaves.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Organ / stage
- leavese0
- Treatment
- jasmonic acid(JA)e0
- Gene
- GhERF8e0
- Outcome
- expression of GhERF8e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under ethephon and jasmonic acid(JA) treatment,the expression of GhERF8 was up-regulated in leaves”
t0“Gossypium hirsutum L.”
Claim · model-written summary
Abscisic acid treatment produced no significant change in GhERF8 expression.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Treatment
- abscisic acid(ABA)e0
- Gene
- GhERF8e0
- Outcome
- expression of GhERF8e0
- Direction
- no significant changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was no significant change in expression of GhERF8 under abscisic acid(ABA) treatment.”
t0“Gossypium hirsutum L.”
Claim · model-written summary
The authors infer that GhERF8 could participate in ethylene and JA signal transduction.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Gene
- GhERF8e0
- Outcome
- signal transduction of ethylene and JAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhERF8 could participate in the signal transduction of ethylene and JA”
t0“Gossypium hirsutum L.”
Claim · model-written summary
The authors infer that GhERF8 does not participate in the ABA-dependent pathway.
- Taxon
- Gossypium hirsutum L.Gossypium hirsutumt0
- Gene
- GhERF8t0
- Outcome
- ABA-dependent pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not in the ABA-dependent pathway.”
t0“GhERF8 Gene in Gossypium hirsutum L.”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
3 papers in the same direction as this paper · 12 in the opposite direction
- positive
- positive
- positive
12 papers more in this group
See all 16 papers →jasmonic acid · molecular: expression · Gossypium hirsutum (cotton)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.