Abstract
Lysobacter antibioticus HS124 is an antagonistic bacterial strain that was previously isolated from the rhizosphere soil of pepper and showed an enhanced ability to produce lytic enzymes as well as an antibiotic that was identified as 4-hydroxyphenylacetic acid (4-HPAA). In the present study, nematicidal activity of the strain and 4-HPAA against the root-knot nematode, Meloidogyne incognita , causing disease in tomato was investigated in both in vitro and in vivo conditions. For this purpose, adding different concentrations of culture filtrate, crude extract collected from extraction with ethyl acetate and 4-HPAA, in 24-well plates containing ca 500 eggs or 300 second-stage juveniles (J2), significantly decreased the rate of nematode hatch and caused higher mortality of J2 compared with the control treatments. Nematicidal activity of the bacterial strain was further confirmed by conducting pot experiments in which tomato plants were inoculated with M. incognita and the HS124 culture (BC). The control pots were treated with commercial nematicide (CN, 5% Ethoprophos), tap water (TW) or the non-inoculated bacterial culture medium (BCM). In these pot experiments, results demonstrated a strong antagonistic potential of L. antibioticus HS124 against M. incognita where the disease was significantly reduced in the pots treated with BC as compared to TW or BCM. Furthermore, the shoot fresh weight was also increased significantly, which may be attributed to the disease control ability of the strain. Hence, L. antibioticus HS124 may be further developed as a potential biocontrol of root knot nematode in the field.
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Claims 5
Claim · model-written summary
Culture filtrate, crude extract and 4-HPAA of the strain decreased nematode hatch compared with controls.
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- culture filtrate, crude extract collected from extraction with ethyl acetate and 4-HPAAe2
- Interactor
- Meloidogyne incognita
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=60)
Meloidogyne incognitae1pest - Second interactor
- Lysobacter antibioticus HS124Lysobacter antibioticus HS124e0biocontrol_agent
- Outcome
- rate of nematode hatche2
- Direction
- decreasede2
- Comparator
- the control treatmentse2
- Setting
- in 24-well platese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=500)
Verbatim
e0“Lysobacter antibioticus HS124 is an antagonistic bacterial strain”
e1“nematicidal activity of the strain and 4-HPAA against the root-knot nematode, Meloidogyne incognita, causing disease in tomato was investigated in both in vitro and in vivo conditions”
e2“adding different concentrations of culture filtrate, crude extract collected from extraction with ethyl acetate and 4-HPAA, in 24-well plates containing ca 500 eggs or 300 second-stage juveniles (J2), significantly decreased the rate of nematode hatch and caused higher mortality of J2 compared with the control treatments”
Claim · model-written summary
Culture filtrate, crude extract and 4-HPAA of the strain caused higher mortality of second-stage juveniles compared with controls.
- Taxon
- tomatoSolanum lycopersicume1
- Organ / stage
- second-stage juveniles (J2)e2
- Treatment
- culture filtrate, crude extract collected from extraction with ethyl acetate and 4-HPAAe2
- Interactor
- Meloidogyne incognitaMeloidogyne incognitae1pest
- Second interactor
- Lysobacter antibioticus HS124Lysobacter antibioticus HS124e0biocontrol_agent
- Outcome
- mortality of J2e2
- Direction
- highere2
- Comparator
- the control treatmentse2
- Setting
- in 24-well platese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lysobacter antibioticus HS124 is an antagonistic bacterial strain”
e1“nematicidal activity of the strain and 4-HPAA against the root-knot nematode, Meloidogyne incognita, causing disease in tomato was investigated in both in vitro and in vivo conditions”
e2“adding different concentrations of culture filtrate, crude extract collected from extraction with ethyl acetate and 4-HPAA, in 24-well plates containing ca 500 eggs or 300 second-stage juveniles (J2), significantly decreased the rate of nematode hatch and caused higher mortality of J2 compared with the control treatments”
Claim · model-written summary
In pots, the strain culture (BC) significantly reduced tomato disease caused by M. incognita compared with TW or BCM.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- BCe3
- Interactor
- M. incognitaMeloidogyne incognitae3pest
- Second interactor
- L. antibioticus HS124Lysobacter antibioticuse3biocontrol_agent
- Outcome
- diseasee3
- Direction
- significantly reducede3
- Comparator
- TW or BCMe3
- Setting
- potse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“nematicidal activity of the strain and 4-HPAA against the root-knot nematode, Meloidogyne incognita, causing disease in tomato was investigated in both in vitro and in vivo conditions”
e3“results demonstrated a strong antagonistic potential of L. antibioticus HS124 against M. incognita where the disease was significantly reduced in the pots treated with BC as compared to TW or BCM”
Claim · model-written summary
Shoot fresh weight of tomato increased significantly, which may be attributed to the disease control ability of the strain.
- Taxon
- tomatoSolanum lycopersicume1
- Interactor
- Meloidogyne incognitaMeloidogyne incognitae1pest
- Outcome
- shoot fresh weighte4
- Direction
- increased significantlye4
- Qualifier
- may be attributed to the disease control ability of the straine4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“nematicidal activity of the strain and 4-HPAA against the root-knot nematode, Meloidogyne incognita, causing disease in tomato was investigated in both in vitro and in vivo conditions”
e4“Furthermore, the shoot fresh weight was also increased significantly, which may be attributed to the disease control ability of the strain”
Claim · model-written summary
L. antibioticus HS124 may be further developed as a field biocontrol agent against root knot nematode on tomato.
- Taxon
- tomatoSolanum lycopersicume1
- Interactor
- root knot nematodeMeloidogynee5pest
- Second interactor
- L. antibioticus HS124Lysobacter antibioticuse5biocontrol_agent
- Outcome
- potential biocontrol of root knot nematodee5
- Setting
- in the fielde5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“nematicidal activity of the strain and 4-HPAA against the root-knot nematode, Meloidogyne incognita, causing disease in tomato was investigated in both in vitro and in vivo conditions”
e5“Hence, L. antibioticus HS124 may be further developed as a potential biocontrol of root knot nematode in the field”
The same questions in other papers
ethyl acetate · Meloidogyne incognita · Solanum lycopersicum (tomato)Results agree
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See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.