Abstract
The establishment of an efficient Agrobacterium–mediated transformation protocol for a recalcitrant crop like high oleic sunflower genotypes, cv. Capella and SWSR2 inbred line, is presented in this paper. The protocol requires the identification and optimization of parameters affecting T-DNA delivery and plant regeneration. The basis for identifying the most appropriate conditions for transformation was the fluorometric, histochemical GUS activity coupled with plant cell vitality. Agrobacterium tumefaciens–mediated transformation was performed using strain LBA4404 for cv. Capella and strain GV3101 for the inbred line. Both strains harboring the pBI121 plasmid with the gus gene were under the control of 35S promoter. The flourometric GUS activity was increased in cv. Capella and SWSR2 inbred line 1.9 and 1.6 fold, respectively, relative to transformation without inducers by optimizing the bacterial density to OD600=1.0, by splitting shoot apices and by using MS medium as a co-cultivation medium supplemented with 200 µM acetosyringone. Pre-culturing the explants for three days on SIM2 medium before the bacterial inoculation followed by co-cultivation for 72 h increased the percentage of the GUS expression to 40% and 30% in cv. Capella and SWSR2 inbred line, respectively. It also improved the regeneration percentage as well as the vitality of cells.
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Claims 5
Claim · model-written summary
Optimized conditions increased fluorometric GUS activity 1.9 fold in cv. Capella relative to transformation without inducers.
- Taxon
- sunflower
Helianthus annuust0 - Material
- cv. Capellae0
- Treatment
- optimizing the bacterial density to OD600=1.0, by splitting shoot apices and by using MS medium as a co-cultivation medium supplemented with 200 µM acetosyringonee0
- Outcome
- flourometric GUS activitye0
- Direction
- increasede0
- Value
- 1.9e0
- Qualifier
- folde0
- Comparator
- transformation without inducerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The flourometric GUS activity was increased in cv. Capella and SWSR2 inbred line 1.9 and 1.6 fold, respectively, relative to transformation without inducers by optimizing the bacterial density to OD600=1.0, by splitting shoot apices and by using MS medium as a co-cultivation medium supplemented with 200 µM acetosyringone.”
t0“sunflower”
Claim · model-written summary
Optimized conditions increased fluorometric GUS activity 1.6 fold in SWSR2 relative to transformation without inducers.
- Taxon
- sunflowerHelianthus annuust0
- Material
- SWSR2 inbred linee0
- Treatment
- optimizing the bacterial density to OD600=1.0, by splitting shoot apices and by using MS medium as a co-cultivation medium supplemented with 200 µM acetosyringonee0
- Outcome
- flourometric GUS activitye0
- Direction
- increasede0
- Value
- 1.6 folde0
- Comparator
- transformation without inducerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The flourometric GUS activity was increased in cv. Capella and SWSR2 inbred line 1.9 and 1.6 fold, respectively, relative to transformation without inducers by optimizing the bacterial density to OD600=1.0, by splitting shoot apices and by using MS medium as a co-cultivation medium supplemented with 200 µM acetosyringone.”
t0“sunflower”
Claim · model-written summary
Pre-culturing and co-cultivation raised GUS expression to 40% in cv. Capella.
- Taxon
- sunflowerHelianthus annuust0
- Material
- cv. Capellae0
- Treatment
- Pre-culturing the explants for three days on SIM2 medium before the bacterial inoculation followed by co-cultivation for 72 he0
- Outcome
- percentage of the GUS expressione0
- Direction
- increasede0
- Value
- 40%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-culturing the explants for three days on SIM2 medium before the bacterial inoculation followed by co-cultivation for 72 h increased the percentage of the GUS expression to 40% and 30% in cv. Capella and SWSR2 inbred line, respectively.”
t0“sunflower”
Claim · model-written summary
Pre-culturing and co-cultivation raised GUS expression to 30% in SWSR2.
- Taxon
- sunflowerHelianthus annuust0
- Material
- SWSR2 inbred linee0
- Treatment
- Pre-culturing the explants for three days on SIM2 medium before the bacterial inoculation followed by co-cultivation for 72 he0
- Outcome
- percentage of the GUS expressione0
- Direction
- increasede0
- Value
- 30%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-culturing the explants for three days on SIM2 medium before the bacterial inoculation followed by co-cultivation for 72 h increased the percentage of the GUS expression to 40% and 30% in cv. Capella and SWSR2 inbred line, respectively.”
t0“sunflower”
Claim · model-written summary
Pre-culturing and co-cultivation improved regeneration percentage and cell vitality.
- Taxon
- sunflowerHelianthus annuust0
- Treatment
- Pre-culturing the explants for three days on SIM2 medium before the bacterial inoculation followed by co-cultivation for 72 he0
- Outcome
- regeneration percentage as well as the vitality of cellse0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pre-culturing the explants for three days on SIM2 medium before the bacterial inoculation followed by co-cultivation for 72 h increased the percentage of the GUS expression to 40% and 30% in cv. Capella and SWSR2 inbred line, respectively. It also improved the regeneration percentage as well as the vitality of cells.”
t0“sunflower”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.