Abstract
Response of sunflower ( Helianthus annuus L.) to in vitro culture has been shown to depend on medium, genotype and explant tissue. The objective of the present study was to evaluate the potential for shoot organogenesis from callus, of globular‐ to torpedo‐stage embryos (0.2–1.2 mm long), of 59 sunflower genotypes. Embryos were cultured on four modified Murashige and Skoog media (MS) varying in concentrations of NAA (naphthalene acetic acid) and BA (6‐benzylaminopurine). The MSO contained 1 mg/L NAA and 0.1 mg/L BA; MSI contained 2 mg/L NAA and 1 mg/L BA; MS2 contained 0.5 mg/L each NAA and BA; and MS3 contained 0.1 mg/L NAA and 1 mg/L BA. Frequency of embryo‐derived callus forming shoots was used to measure potential for plant regeneration. Five genotypes produced no callus or shoots from callus on any media, and both callus and shoot formation occurred on 5% or less of the plated embryos of eight genotypes. Three genotypes showed 80% or more callus formation with little or no shoot organogenesis; whereas, for four genotypes, more than 70% of the plated embryos developed shoots from callus on one or more media. One‐half of the genotypes showed 20% or more shoot‐forming callus. Effect of the medium varied in importance. All media were equivalent for callus formation, but MS3 had the most genotypes with a high frequency of shoot formation from callus. MS2 was second in promoting shoot organogenesis from callus. Because of a strong genotype ✕ medium interaction, the use of two MS media with different hormonal compositions is recommended to increase the frequency of sunflower genotypes regenerating shoots from callus derived from globular‐ to torpedo‐shaped embryos.
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Claims 9
Claim · model-written summary
Five sunflower genotypes produced no callus or shoots from callus on any medium.
- Taxon
- Helianthus annuus
Helianthus annuuse0 - Material
- Five genotypese1
- Organ / stage
- globular‐ to torpedo‐stage embryose2
- Outcome
- callus or shootse1
- Qualifier
- on any mediae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sunflower (Helianthus annuus L.)”
e1“Five genotypes produced no callus or shoots from callus on any media”
e2“of globular‐ to torpedo‐stage embryos”
Claim · model-written summary
Three sunflower genotypes showed 80% or more callus formation with little or no shoot organogenesis.
- Taxon
- Helianthus annuusHelianthus annuuse0
- Material
- Three genotypese1
- Organ / stage
- globular‐ to torpedo‐stage embryose2
- Outcome
- callus formatione1
- Value
- 80% or moree1
- Qualifier
- little or no shoot organogenesise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sunflower (Helianthus annuus L.)”
e1“Three genotypes showed 80% or more callus formation with little or no shoot organogenesis”
e2“of globular‐ to torpedo‐stage embryos”
Claim · model-written summary
For four sunflower genotypes, more than 70% of plated embryos developed shoots from callus on one or more media.
- Taxon
- Helianthus annuusHelianthus annuuse0
- Material
- four genotypese1
- Outcome
- developed shoots from calluse1
- Value
- more than 70%e1
- Qualifier
- on one or more mediae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sunflower (Helianthus annuus L.)”
e1“for four genotypes, more than 70% of the plated embryos developed shoots from callus on one or more media”
Claim · model-written summary
One-half of the sunflower genotypes showed 20% or more shoot-forming callus.
- Taxon
- Helianthus annuusHelianthus annuuse0
- Material
- the genotypese1
- Outcome
- shoot‐forming calluse1
- Value
- 20% or moree1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sunflower (Helianthus annuus L.)”
e1“One‐half of the genotypes showed 20% or more shoot‐forming callus”
Claim · model-written summary
Medium MS3 gave the most sunflower genotypes with a high frequency of shoot formation from callus.
- Taxon
- Helianthus annuusHelianthus annuuse0
- Treatment
- MS3e1
- Outcome
- shoot formation from calluse1
- Direction
- moste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sunflower (Helianthus annuus L.)”
e1“MS3 had the most genotypes with a high frequency of shoot formation from callus”
Claim · model-written summary
Medium MS2 was second in promoting shoot organogenesis from callus.
- Taxon
- Helianthus annuusHelianthus annuuse0
- Treatment
- MS2e1
- Outcome
- shoot organogenesis from calluse1
- Direction
- second in promotinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sunflower (Helianthus annuus L.)”
e1“MS2 was second in promoting shoot organogenesis from callus”
Claim · model-written summary
All media were equivalent for callus formation of sunflower embryos.
- Taxon
- Helianthus annuusHelianthus annuuse0
- Treatment
- mediae1
- Outcome
- callus formatione1
- Direction
- equivalente1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sunflower (Helianthus annuus L.)”
e1“All media were equivalent for callus formation”
Claim · model-written summary
A strong genotype x medium interaction affected sunflower shoot regeneration from callus.
- Taxon
- Helianthus annuusHelianthus annuuse0
- Outcome
- genotype ✕ medium interactione1
- Qualifier
- stronge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sunflower (Helianthus annuus L.)”
e1“Because of a strong genotype ✕ medium interaction”
Claim · model-written summary
Two MS media with different hormonal compositions are recommended to increase the frequency of sunflower genotypes regenerating shoots from callus.
- Taxon
- sunflowerHelianthus annuuse1
- Outcome
- frequency of sunflower genotypes regenerating shoots from calluse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the use of two MS media with different hormonal compositions is recommended to increase the frequency of sunflower genotypes regenerating shoots from callus”
The same questions in other papers
in vitro · growth · Helianthus annuus (common sunflower)Positive and negative results
13 papers in the same direction as this paper · 22 in the opposite direction
- positive
- positive
- positive
32 papers more in this group
See all 36 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.