Paper · 1977 · Physiologia Plantarum

Abscisic Acid and the Germination of Thermodormant Lettuce Fruits (Lactuca sativa cv. Grand Rapids). The Fate of Isotopically Labelled Abscisic Acid

J. ROBERTSON; A. M. M. BERRIE

11
claims
20
verbatim quotes
13
citations
1
research areas
—
Not open access

Abstract

Abstract ABA inhibits the germination of Grand Rapids lettuce seeds at a temperature at which photoblastism is not observed. This ABA inhibition can be overcome by GA, or cytokinin, or red light acting alone or in concert. At high levels of ABA the ameliorative effects of GA, cytokinin or red light are not manifest and it is suggested that lettuce seeds' response to a given level of ABA is temperature dependent, and any interaction which may be observed between ABA and other regulators, or red light, likewise is temperature dependent. Seeds only actively metabolize ABA after germination is complete though ABA does influence the germination process proper. Some radioactivity, originally applied as 14 C ABA, cannot be extracted from 14 C ABA treated seeds by methanol, and this “fixed” ABA can constitute more than 50% of the activity taken up by the seeds. High levels of “fixed” ABA are found in seeds which do not germinate. As well as this “fixed” ABA two labelled metabolites arise in seeds treated with 14 C ABA. The levels of these rise in seed extracts as the level of extractable ABA decreases. Whilst ABA can affect germination our results do not allow us to assign a specific regulatory role to ABA in seed germination.

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Claims 11

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

ABA inhibits lettuce seed germination at a temperature at which photoblastism is not observed.

Taxon
lettuceLactuca sativae0
Organ / stage
seedse0
Treatment
ABAe0
Outcome
germinatione0
Direction
inhibitse0
Qualifier
at a temperature at which photoblastism is not observede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“ABA inhibits the germination of Grand Rapids lettuce seeds at a temperature at which photoblastism is not observed”

✓ located verbatim in the source · abstract, characters 9–123 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The ABA inhibition of lettuce seed germination can be overcome by GA, cytokinin, or red light.

Taxon
lettuceLactuca sativae0
Organ / stage
seedse0
Treatment
GA, or cytokinin, or red lighte1
Outcome
ABA inhibitione1
Direction
overcomee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“ABA inhibits the germination of Grand Rapids lettuce seeds at a temperature at which photoblastism is not observed”

✓ located verbatim in the source · abstract, characters 9–123 · show in text ↑
e1

“This ABA inhibition can be overcome by GA, or cytokinin, or red light acting alone or in concert”

✓ located verbatim in the source · abstract, characters 125–221 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

At high levels of ABA the ameliorative effects of GA, cytokinin or red light are not manifest in lettuce seeds.

Taxon
lettuceLactuca sativae0
Treatment
high levels of ABAe2
Outcome
ameliorative effects of GA, cytokinin or red lighte2
Direction
not manifeste2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“ABA inhibits the germination of Grand Rapids lettuce seeds at a temperature at which photoblastism is not observed”

✓ located verbatim in the source · abstract, characters 9–123 · show in text ↑
e2

“At high levels of ABA the ameliorative effects of GA, cytokinin or red light are not manifest”

✓ located verbatim in the source · abstract, characters 223–316 · show in text ↑
Associationhypothesisoriginal resultmeasured

Claim · model-written summary

Lettuce seed response to a given level of ABA is suggested to be temperature dependent.

Taxon
lettuceLactuca sativae3
Treatment
temperaturee3
Outcome
response to a given level of ABAe3

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e3

“it is suggested that lettuce seeds' response to a given level of ABA is temperature dependent”

✓ located verbatim in the source · abstract, characters 321–414 · show in text ↑
Associationhypothesisoriginal resultmeasured

Claim · model-written summary

Any interaction between ABA and other regulators or red light is suggested to be temperature dependent.

Taxon
lettuceLactuca sativae0
Treatment
temperaturee4
Outcome
interaction which may be observed between ABA and other regulatorse4

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“ABA inhibits the germination of Grand Rapids lettuce seeds at a temperature at which photoblastism is not observed”

✓ located verbatim in the source · abstract, characters 9–123 · show in text ↑
e4

“any interaction which may be observed between ABA and other regulators, or red light, likewise is temperature dependent”

✓ located verbatim in the source · abstract, characters 420–539 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Lettuce seeds actively metabolize ABA only after germination is complete.

Taxon
lettuceLactuca sativae0
Organ / stage
Seedse5
Outcome
actively metabolize ABAe5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“ABA inhibits the germination of Grand Rapids lettuce seeds at a temperature at which photoblastism is not observed”

✓ located verbatim in the source · abstract, characters 9–123 · show in text ↑
e5

“Seeds only actively metabolize ABA after germination is complete”

✓ located verbatim in the source · abstract, characters 541–605 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Fixed ABA can constitute more than 50% of the radioactivity taken up by lettuce seeds treated with 14C ABA.

Taxon
lettuceLactuca sativae0
Treatment
14 C ABAe6
Outcome
activity taken up by the seedse6
Value
more than 50%e6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“ABA inhibits the germination of Grand Rapids lettuce seeds at a temperature at which photoblastism is not observed”

✓ located verbatim in the source · abstract, characters 9–123 · show in text ↑
e6

“Some radioactivity, originally applied as 14 C ABA, cannot be extracted from 14 C ABA treated seeds by methanol, and this “fixed” ABA can constitute more than 50% of the activity taken up by the seeds”

✓ located verbatim in the source · abstract, characters 664–864 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

High levels of fixed ABA are found in lettuce seeds which do not germinate.

Taxon
lettuceLactuca sativae0
Organ / stage
seeds which do not germinatee7
Outcome
High levelse7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“ABA inhibits the germination of Grand Rapids lettuce seeds at a temperature at which photoblastism is not observed”

✓ located verbatim in the source · abstract, characters 9–123 · show in text ↑
e7

“High levels of “fixed” ABA are found in seeds which do not germinate”

✓ located verbatim in the source · abstract, characters 866–934 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Two labelled metabolites arise in lettuce seeds treated with 14C ABA.

Taxon
lettuceLactuca sativae0
Organ / stage
seedse7
Treatment
14 C ABAe7
Outcome
two labelled metabolitese7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“ABA inhibits the germination of Grand Rapids lettuce seeds at a temperature at which photoblastism is not observed”

✓ located verbatim in the source · abstract, characters 9–123 · show in text ↑
e7

“two labelled metabolites arise in seeds treated with 14 C ABA”

✓ located verbatim in the source · abstract, characters 964–1025 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Levels of the labelled metabolites rise in seed extracts as extractable ABA decreases.

Taxon
lettuceLactuca sativae0
Compound
extractable ABAe6
Outcome
levels of thesee6
Direction
risee6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“ABA inhibits the germination of Grand Rapids lettuce seeds at a temperature at which photoblastism is not observed”

✓ located verbatim in the source · abstract, characters 9–123 · show in text ↑
e6

“The levels of these rise in seed extracts as the level of extractable ABA decreases”

✓ located verbatim in the source · abstract, characters 1027–1110 · show in text ↑
Associationauthor interpretationoriginal resultmeasured

Claim · model-written summary

The results do not allow assigning a specific regulatory role to ABA in seed germination, although ABA can affect germination.

Taxon
lettuceLactuca sativae0
Compound
ABAe5
Outcome
germinatione5

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“ABA inhibits the germination of Grand Rapids lettuce seeds at a temperature at which photoblastism is not observed”

✓ located verbatim in the source · abstract, characters 9–123 · show in text ↑
e5

“Whilst ABA can affect germination our results do not allow us to assign a specific regulatory role to ABA in seed germination”

✓ located verbatim in the source · abstract, characters 1112–1237 · show in text ↑

Gibberellic acid · disease · Lactuca sativa (garden lettuce)Positive and negative results

6 papers: 4 positive, 2 negative or no effect

3 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

2 papers more in this group

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(+)-abscisic acid · growth · Lactuca sativa (garden lettuce)Positive and negative results

11 papers: 2 positive, 9 negative or no effect

8 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

7 papers more in this group

See all 11 papers →

light · disease · Lactuca sativa (garden lettuce)Positive and negative results

22 papers: 13 positive, 9 negative or no effect

12 papers in the same direction as this paper · 9 in the opposite direction

  1. negative
  2. negative
  3. negative

18 papers more in this group

See all 22 papers →

class · disease · Lactuca sativa (garden lettuce)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.