Abstract
NADH oxidation by plasma membrane vesicles purified from hypocotyls of etiolated soybean seedlings by two-phase partition was stimulated 2- to 3-fold by auxins, indole-3-acetic acid, 2,4-dichlorophenoxy acetic acid (2,4-D), and alpha-naphthaleneacetic acid. The stimulation was concentration dependent in the presence or absence of detergent with a maximum for 2,4-D at 1 micromolar. The NADH oxidation activity was solubilized with the zwitterionic detergent CHAPS and purified by ion exchange chromatography and gel filtration approximately 2000-fold over the total homogenate. Both the partially purified fraction and an active band from nondenaturing gel electrophoresis revealed the same three bands when analyzed by denaturing gel electrophoresis. When obtained from plasma membrane vesicles from the region of rapid cell elongation, the NADH oxidase complex retained auxin responsiveness throughout purification (3- to 5-fold stimulation by 1 micromolar 2,4-D).
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Claims 5
Claim · model-written summary
Auxins stimulated soybean membrane NADH oxidation two- to threefold.
- Taxon
- soybean
Glycine maxe0 - Organ / stage
- hypocotylse0
- Treatment
- auxinse0
- Outcome
- NADH oxidatione0
- Direction
- stimulatede0
- Value
- 2- to 3-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NADH oxidation by plasma membrane vesicles purified from hypocotyls of etiolated soybean seedlings by two-phase partition was stimulated 2- to 3-fold by auxins, indole-3-acetic acid, 2,4-dichlorophenoxy acetic acid (2,4-D), and alpha-naphthaleneacetic acid”
Claim · model-written summary
Stimulation was concentration dependent, with a maximum for 2,4-D at 1 micromolar.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- stimulatione0
- Direction
- maximume0
- Value
- 1 micromolare0
- Qualifier
- in the presence or absence of detergente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The stimulation was concentration dependent in the presence or absence of detergent with a maximum for 2,4-D at 1 micromolar”
t0“Soybean”
Claim · model-written summary
NADH oxidation activity was purified approximately 2000-fold.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- NADH oxidation activitye0
- Value
- approximately 2000-folde0
- Comparator
- total homogenatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The NADH oxidation activity was solubilized with the zwitterionic detergent CHAPS and purified by ion exchange chromatography and gel filtration approximately 2000-fold over the total homogenate”
t0“Soybean”
Claim · model-written summary
The partially purified fraction and active electrophoresis band each revealed the same three bands.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- same three bandse0
- Value
- three bandse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both the partially purified fraction and an active band from nondenaturing gel electrophoresis revealed the same three bands when analyzed by denaturing gel electrophoresis”
t0“Soybean”
Claim · model-written summary
The NADH oxidase complex retained auxin responsiveness through purification, with three- to fivefold stimulation by 2,4-D.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- 1 micromolar 2,4-De0
- Outcome
- NADH oxidase complexe0
- Direction
- stimulatione0
- Value
- 3- to 5-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the NADH oxidase complex retained auxin responsiveness throughout purification (3- to 5-fold stimulation by 1 micromolar 2,4-D)”
t0“Soybean”
The same questions in other papers
2,4-D · molecular: metabolites · Glycine max (soybean)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
2 papers more in this group
See all 6 papers →class · molecular: metabolites · Glycine max (soybean)Positive and negative results
10 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.