Abstract
An auxin-requiring soybean root callus metabolized [1-(14)C]-2,4-dichlorophenoxyacetic acid (2,4-D) to diethyl ether-soluble amino acid conjugates and water-soluble metabolites. The uptake in tissue varied with incubation time, concentration, and amount of tissue. Uptake was essentially complete (80%) after a 24-hour incubation and the percentage of free 2,4-D in the tissue fell to its lowest point at this time. At later times, the percentage of free 2,4-D increased and the percentage of amino acid conjugates decreased, whereas the percentage of water-soluble metabolites increased only slightly. Similar trends were seen if the tissue was incubated for 24 hours in radioactive 2,4-D, followed by incubation in media without 2,4-D for 24 hours. Inclusion of nonlabeled 2,4-D during the 24-hour chase period did not reduce amino acid conjugate disappearance but did reduce the percentage of free [1-(14)C]2,4-D. Thus, an external supply of 2,4-D does not directly prevent amino acid conjugate metabolism in this tissue. It is concluded that 2,4-D amino acid conjugates were actively metabolized by this tissue to free 2,4-D and water-soluble metabolites.
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Claims 9
Claim · model-written summary
Soybean root callus metabolized 2,4-D to diethyl ether-soluble amino acid conjugates and water-soluble metabolites.
- Taxon
- soybean
Glycine maxe1 - Organ / stage
- root calluse1
- Compound
- 2,4-De1
- Outcome
- diethyl ether-soluble amino acid conjugatese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“An auxin-requiring soybean root callus metabolized [1-(14)C]-2,4-dichlorophenoxyacetic acid (2,4-D) to diethyl ether-soluble amino acid conjugates and water-soluble metabolites”
Claim · model-written summary
2,4-D uptake into soybean root callus was essentially complete (80%) after a 24-hour incubation.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- root calluse1
- Treatment
- 24-hour incubatione2
- Compound
- 2,4-De1
- Outcome
- Uptakee2
- Value
- 80%e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“An auxin-requiring soybean root callus metabolized [1-(14)C]-2,4-dichlorophenoxyacetic acid (2,4-D) to diethyl ether-soluble amino acid conjugates and water-soluble metabolites”
e2“Uptake was essentially complete (80%) after a 24-hour incubation and the percentage of free 2,4-D in the tissue fell to its lowest point at this time”
Claim · model-written summary
The percentage of free 2,4-D in soybean root callus fell to its lowest point at 24 hours.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- root calluse1
- Treatment
- 24-hour incubatione2
- Compound
- 2,4-De1
- Outcome
- percentage of free 2,4-D in the tissuee2
- Direction
- felle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“An auxin-requiring soybean root callus metabolized [1-(14)C]-2,4-dichlorophenoxyacetic acid (2,4-D) to diethyl ether-soluble amino acid conjugates and water-soluble metabolites”
e2“Uptake was essentially complete (80%) after a 24-hour incubation and the percentage of free 2,4-D in the tissue fell to its lowest point at this time”
Claim · model-written summary
At later times, the percentage of free 2,4-D in soybean root callus increased.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- root calluse1
- Compound
- 2,4-De1
- Outcome
- percentage of free 2,4-De3
- Direction
- increasede3
- Qualifier
- At later timese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“An auxin-requiring soybean root callus metabolized [1-(14)C]-2,4-dichlorophenoxyacetic acid (2,4-D) to diethyl ether-soluble amino acid conjugates and water-soluble metabolites”
e3“At later times, the percentage of free 2,4-D increased and the percentage of amino acid conjugates decreased, whereas the percentage of water-soluble metabolites increased only slightly”
Claim · model-written summary
At later times, the percentage of amino acid conjugates in soybean root callus decreased.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- root calluse1
- Compound
- 2,4-De1
- Outcome
- percentage of amino acid conjugatese3
- Direction
- decreasede3
- Qualifier
- At later timese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“An auxin-requiring soybean root callus metabolized [1-(14)C]-2,4-dichlorophenoxyacetic acid (2,4-D) to diethyl ether-soluble amino acid conjugates and water-soluble metabolites”
e3“At later times, the percentage of free 2,4-D increased and the percentage of amino acid conjugates decreased, whereas the percentage of water-soluble metabolites increased only slightly”
Claim · model-written summary
At later times, the percentage of water-soluble metabolites in soybean root callus increased only slightly.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- root calluse1
- Compound
- 2,4-De1
- Outcome
- percentage of water-soluble metabolitese3
- Direction
- increased only slightlye3
- Qualifier
- At later timese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“An auxin-requiring soybean root callus metabolized [1-(14)C]-2,4-dichlorophenoxyacetic acid (2,4-D) to diethyl ether-soluble amino acid conjugates and water-soluble metabolites”
e3“At later times, the percentage of free 2,4-D increased and the percentage of amino acid conjugates decreased, whereas the percentage of water-soluble metabolites increased only slightly”
Claim · model-written summary
Nonlabeled 2,4-D added during the chase did not reduce disappearance of amino acid conjugates in soybean root callus.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- root calluse1
- Treatment
- Inclusion of nonlabeled 2,4-D during the 24-hour chase periode4
- Compound
- 2,4-De1
- Outcome
- amino acid conjugate disappearancee4
- Direction
- did not reducee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“An auxin-requiring soybean root callus metabolized [1-(14)C]-2,4-dichlorophenoxyacetic acid (2,4-D) to diethyl ether-soluble amino acid conjugates and water-soluble metabolites”
e4“Inclusion of nonlabeled 2,4-D during the 24-hour chase period did not reduce amino acid conjugate disappearance but did reduce the percentage of free [1-(14)C]2,4-D”
Claim · model-written summary
Nonlabeled 2,4-D added during the chase reduced the percentage of free labeled 2,4-D in soybean root callus.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- root calluse1
- Treatment
- Inclusion of nonlabeled 2,4-D during the 24-hour chase periode4
- Compound
- 2,4-De1
- Outcome
- percentage of free [1-(14)C]2,4-De4
- Direction
- reducee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“An auxin-requiring soybean root callus metabolized [1-(14)C]-2,4-dichlorophenoxyacetic acid (2,4-D) to diethyl ether-soluble amino acid conjugates and water-soluble metabolites”
e4“Inclusion of nonlabeled 2,4-D during the 24-hour chase period did not reduce amino acid conjugate disappearance but did reduce the percentage of free [1-(14)C]2,4-D”
Claim · model-written summary
Soybean root callus actively metabolizes 2,4-D amino acid conjugates to free 2,4-D and water-soluble metabolites.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- root calluse1
- Compound
- 2,4-D amino acid conjugatese5
- Outcome
- actively metabolizede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“An auxin-requiring soybean root callus metabolized [1-(14)C]-2,4-dichlorophenoxyacetic acid (2,4-D) to diethyl ether-soluble amino acid conjugates and water-soluble metabolites”
e5“It is concluded that 2,4-D amino acid conjugates were actively metabolized by this tissue to free 2,4-D and water-soluble metabolites”
The same questions in other papers
2,4-D · molecular: metabolites · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.