Abstract
1-Aminocyclopropane-1-carboxylate (ACC) deaminase is commonly produced by plant growth-promoting rhizobacteria (PGPR) and has been suggested to facilitate the growth and stress tolerance of hosts via a reduction in levels of ethylene. However, the regulatory mechanism of ACC deaminase (AcdS) protein within host plant cells is largely unknown. Here, we demonstrated beneficial effects and post-translational modification of PGPR-originated AcdS proteins in plants. Compared with the wild-type, transgenic Arabidopsis expressing the Pseudomonas fluorescens acdS (PfacdS) gene displayed increased root elongation and reduced sensitivity to 10 μM exogenous ACC, an ethylene precursor. Arabidopsis expressing PfacdS also showed increased tolerance to high salinity (150 mM NaCl). PfAcdS proteins accumulated in transgenic Arabidopsis were rapidly degraded, which was potentially mediated by the 26S proteasome pathway. The degradation of PfAcdS was alleviated in the presence of exogenous ACC. In conclusion, our data suggest that the plant growth-promoting effects of bacterial AcdS proteins are potentially modulated via protein turnover inside the host plant cells. Such post-translational modification plays a physiological role in the mutualistic interactions between microorganisms and plants in the rhizospheric and/or endospheric niche.
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Claims 7
Claim · model-written summary
PfacdS expression increased Arabidopsis root elongation relative to wild type.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- transgenic Arabidopsis expressing the Pseudomonas fluorescens acdS (PfacdS) genee0
- Gene
- Pseudomonas fluorescens acdS (PfacdS)e0
- Outcome
- root elongatione0
- Direction
- increasede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with the wild-type, transgenic Arabidopsis expressing the Pseudomonas fluorescens acdS (PfacdS) gene displayed increased root elongation and reduced sensitivity to 10 μM exogenous ACC, an ethylene precursor.”
Claim · model-written summary
PfacdS expression reduced Arabidopsis sensitivity to 10 μM exogenous ACC relative to wild type.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- transgenic Arabidopsis expressing the Pseudomonas fluorescens acdS (PfacdS) genee0
- Gene
- Pseudomonas fluorescens acdS (PfacdS)e0
- Outcome
- sensitivity to 10 μM exogenous ACCe0
- Direction
- reducede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with the wild-type, transgenic Arabidopsis expressing the Pseudomonas fluorescens acdS (PfacdS) gene displayed increased root elongation and reduced sensitivity to 10 μM exogenous ACC, an ethylene precursor.”
Claim · model-written summary
Arabidopsis expressing PfacdS had increased tolerance to 150 mM NaCl.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- expressing PfacdSe0
- Gene
- PfacdSe0
- Outcome
- tolerance to high salinitye0
- Direction
- increasede0
- Value
- 150 mM NaCle0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis expressing PfacdS also showed increased tolerance to high salinity (150 mM NaCl).”
Claim · model-written summary
PfAcdS proteins accumulated in transgenic Arabidopsis were rapidly degraded.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- PfAcdS proteinse0
- Outcome
- PfAcdS proteins accumulated in transgenic Arabidopsis were rapidly degradede0
- Direction
- rapidly degradede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PfAcdS proteins accumulated in transgenic Arabidopsis were rapidly degraded, which was potentially mediated by the 26S proteasome pathway.”
Claim · model-written summary
The authors proposed potential mediation of PfAcdS degradation by the 26S proteasome pathway.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- PfAcdS proteinse0
- Outcome
- PfAcdS proteins accumulated in transgenic Arabidopsis were rapidly degradede0
- Qualifier
- 26S proteasome pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PfAcdS proteins accumulated in transgenic Arabidopsis were rapidly degraded, which was potentially mediated by the 26S proteasome pathway.”
Claim · model-written summary
Exogenous ACC alleviated PfAcdS degradation in Arabidopsis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- exogenous ACCe0
- Gene
- PfAcdSe0
- Outcome
- degradation of PfAcdSe0
- Direction
- alleviatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The degradation of PfAcdS was alleviated in the presence of exogenous ACC.”
t0“transgenicArabidopsis”
Claim · model-written summary
Bacterial AcdS growth promotion may be modulated by protein turnover in host plant cells.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- bacterial AcdS proteinse0
- Outcome
- plant growth-promoting effectse0
- Qualifier
- protein turnover inside the host plant cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“our data suggest that the plant growth-promoting effects of bacterial AcdS proteins are potentially modulated via protein turnover inside the host plant cells.”
t0“transgenicArabidopsis”
The same questions in other papers
Pseudomonas fluorescens · disease · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.