Paper · 1974 · PLANT PHYSIOLOGY

Carbon Dioxide Fixation in the Light and in the Dark by Isolated Spinach Chloroplasts

Mordhay Avron; Martin Gibbs

4
claims
7
verbatim quotes
44
citations
1
research areas
✓
Open access

Abstract

Factors affecting CO(2) fixation in the spinach (Spinacia oleracea) chloroplast were investigated. Free magnesium ions are shown to be highly inhibitory for photosynthetic CO(2) fixation in isolated intact spinach chloroplasts. The pH optimum for CO(2) fixation is about 8.5 but is dependent upon the reaction medium. Conditions are defined under which chloroplasts illuminated in the absence of CO(2) accumulate ribulose 1,5-diphosphate, and fix CO(2) in a subsequent dark period when high magnesium ion concentrations are provided. The regulation of photosynthetic CO(2) assimilation by these factors is discussed.

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Claims 4

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Free magnesium ions strongly inhibited photosynthetic carbon dioxide fixation in isolated intact spinach chloroplasts.

Organ / stage
chloroplastse0
Treatment
Free magnesium ionse0
Outcome
photosynthetic CO(2) fixatione0
Direction
highly inhibitorye0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Free magnesium ions are shown to be highly inhibitory for photosynthetic CO(2) fixation in isolated intact spinach chloroplasts.”

✓ located verbatim in the source · abstract, characters 99–227 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The pH optimum for spinach chloroplast carbon dioxide fixation was about 8.5 and depended on reaction medium.

Outcome
pH optimum for CO(2) fixatione0
Value
8.5e0
Qualifier
dependent upon the reaction mediume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The pH optimum for CO(2) fixation is about 8.5 but is dependent upon the reaction medium.”

✓ located verbatim in the source · abstract, characters 228–317 · show in text ↑
t0

“Spinach”

✓ located verbatim in the source · title, characters 65–72 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Illuminated chloroplasts without carbon dioxide accumulated ribulose 1,5-diphosphate under defined conditions.

Organ / stage
chloroplastse0
Treatment
illuminated in the absence of CO(2)e0
Outcome
accumulate ribulose 1,5-diphosphatee0
Direction
accumulatee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“chloroplasts illuminated in the absence of CO(2) accumulate ribulose 1,5-diphosphate”

✓ located verbatim in the source · abstract, characters 353–437 · show in text ↑
t0

“Spinach”

✓ located verbatim in the source · title, characters 65–72 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Under those conditions, spinach chloroplasts fixed carbon dioxide in a subsequent dark period with high magnesium ion concentrations.

Outcome
fix CO(2)e0
Qualifier
in a subsequent dark period when high magnesium ion concentrations are providede0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“fix CO(2) in a subsequent dark period when high magnesium ion concentrations are provided.”

✓ located verbatim in the source · abstract, characters 443–533 · show in text ↑
t0

“Spinach”

✓ located verbatim in the source · title, characters 65–72 · show in text ↑

magnesium ion · physiology: photosynthesis · Spinacia oleracea (spinach)Results agree

2 papers: 0 positive, 2 negative or no effect

1 paper in the same direction as this paper

  1. negative
See all 2 papers →

co2 · molecular: metabolites · Spinacia oleracea (spinach)Positive and negative results

6 papers: 3 positive, 3 negative or no effect

2 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. no effect
  3. negative

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.