Paper · 1969 · PLANT PHYSIOLOGY

The Effect of Salinity on the Malic Dehydrogenase of Pea Roots

Edna Hason-Porath; Alexandra Poljakoff-Mayber

7
claims
14
verbatim quotes
43
citations
1
research areas
✓
Open access

Abstract

Effect of salinity on malate dehydrogenase activity was studied.Pea root tips contain 2 different malate dehydrogenases. One is located in the particulate, mitochondrial fraction, the other in the soluble, cytoplasmic fraction. Both can act when coupled with either NAD or NADP.Growing plants in Na(2)SO(4) salinated medium did not affect the pattern of the malate dehydrogenases in the root tips. Growing plants in NaCl salinated media resulted in the appearance of a new, third isoenzyme. This new isoenzyme was located in the cytoplasmic fraction.Salinity of both types, when present in growth medium, induced increases in the NADP coupled activity of the mitochondrial malate dehydrogenase. The NAD coupled activity, however, was depressed except in the cytoplasmic fraction of plants grown in media salinated with NaCl to 1 atmosphere. Addition of either of the salts to assay media of enzymes, isolated from plants grown in non salinated substrate, did not have any significant effect.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

Pea root tips contain two malate dehydrogenases in mitochondrial and cytoplasmic fractions.

Organ / stage
root tipse0
Gene
malate dehydrogenasese0
Outcome
2 different malate dehydrogenasese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Pea Roots”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
e0

“Pea root tips contain 2 different malate dehydrogenases. One is located in the particulate, mitochondrial fraction, the other in the soluble, cytoplasmic fraction”

✓ located verbatim in the source · abstract, characters 64–226 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Both pea root enzymes can act coupled with NAD or NADP.

Outcome
act when coupled with either NAD or NADPe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Pea Roots”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
e0

“Both can act when coupled with either NAD or NADP”

✓ located verbatim in the source · abstract, characters 228–277 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Na(2)SO(4) salinity did not affect the malate dehydrogenase pattern in pea root tips.

Organ / stage
root tipse0
Treatment
Na(2)SO(4) salinated mediume0
Outcome
pattern of the malate dehydrogenasese0
Direction
did not affecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Pea Roots”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
e0

“Growing plants in Na(2)SO(4) salinated medium did not affect the pattern of the malate dehydrogenases in the root tips”

✓ located verbatim in the source · abstract, characters 278–396 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

NaCl salinity induced a third cytoplasmic isoenzyme in pea roots.

Treatment
NaCl salinated mediae0
Outcome
appearance of a new, third isoenzymee0
Qualifier
cytoplasmic fractione0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Pea Roots”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
e0

“Growing plants in NaCl salinated media resulted in the appearance of a new, third isoenzyme. This new isoenzyme was located in the cytoplasmic fraction”

✓ located verbatim in the source · abstract, characters 398–549 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Both salinity types increased NADP coupled mitochondrial malate dehydrogenase activity.

Treatment
Salinity of both typese0
Outcome
NADP coupled activity of the mitochondrial malate dehydrogenasee0
Direction
increasese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Pea Roots”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
e0

“Salinity of both types, when present in growth medium, induced increases in the NADP coupled activity of the mitochondrial malate dehydrogenase”

✓ located verbatim in the source · abstract, characters 550–693 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Salinity depressed NAD coupled activity except in the cytoplasmic fraction under NaCl at one atmosphere.

Treatment
NaCl to 1 atmospheree0
Outcome
NAD coupled activitye0
Direction
depressede0
Qualifier
except in the cytoplasmic fractione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Pea Roots”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
e0

“The NAD coupled activity, however, was depressed except in the cytoplasmic fraction of plants grown in media salinated with NaCl to 1 atmosphere”

✓ located verbatim in the source · abstract, characters 695–839 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Adding either salt directly to assays did not significantly affect enzymes from unsalinated plants.

Treatment
Addition of either of the salts to assay mediae0
Outcome
enzymese0
Direction
did not have any significant effecte0
Comparator
non salinated substratee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lathyrus oleraceus
Lathyrus oleraceus · TaxonRenee Comet (photographer) AV Number: AV-9400-4164 · Public domain · Wikimedia Commons ↗

Verbatim

t0

“Pea Roots”

✓ located verbatim in the source · title, characters 53–62 · show in text ↑
e0

“Addition of either of the salts to assay media of enzymes, isolated from plants grown in non salinated substrate, did not have any significant effect”

✓ located verbatim in the source · abstract, characters 841–990 · show in text ↑

in vitro · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results

10 papers: 3 positive, 7 negative or no effect

6 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. positive
  3. positive

6 papers more in this group

See all 10 papers →

salinity · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results

15 papers: 1 positive, 14 negative or no effect

14 in the opposite direction

  1. no effect
  2. no effect
  3. negative

11 papers more in this group

See all 15 papers →

in vitro · physiology: enzyme activity · Lathyrus oleraceus (pea)Results agree

4 papers: 0 positive, 4 negative or no effect

3 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative
See all 4 papers →

salinity · physiology: enzyme activity · Lathyrus oleraceus (pea)Results agree

6 papers: 0 positive, 6 negative or no effect

5 papers in the same direction as this paper

  1. negative
  2. negative
  3. no effect

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.