Abstract
Transformation of cantaloupes with the coat protein (cp) gene of papaya ringspot virus type W (PRSV-W), Thai isolate, was used to introduce virus resistance. Binary vectors containing either the full length coat protein coding region under control of the 35S CaMV promoter(pSA1175), or the inverted-repeat of a coat protein coding region (pSA1304), were constructed and used for Agrobacterium-mediated transformation of cotyledonary explants of the cantaloupe cultivar Sun Lady. Four independent transgenic lines were obtained using pSA1304 and one using pSA1175. Integration of the PRSV-W cp gene into the genome of these transgenic lines was verified by PCR amplification, GUS assays and Southern blot hybridization. In vitro inoculation of these lines with PRSV-W revealed that whereas the line containing pSA1175 remained sensitive, the four lines containing pSA1304 were resistant. The presence of small RNA species, presumably siRNA, corresponding to regions of the viral cp gene in transgenic lines resistant to PRSV-W supports the involvement of post-transcriptional gene silencing in the establishment of resistance.
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Claims 6
Claim · model-written summary
Four independent pSA1304 cantaloupe transgenic lines and one pSA1175 line were obtained.
- Taxon
- cantaloupe
Cucumis melo var. cantalupoe1 - Material
- Sun Ladye1
- Outcome
- transgenic linese0
- Value
- Foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four independent transgenic lines were obtained using pSA1304 and one using pSA1175”
e1“cantaloupe cultivar Sun Lady”
Claim · model-written summary
The PRSV-W coat protein gene was integrated into the cantaloupe transgenic lines.
- Taxon
- cantaloupeCucumis melo var. cantalupoe1
- Gene
- PRSV-W cp genee0
- Outcome
- Integration of the PRSV-W cp genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Integration of the PRSV-W cp gene into the genome of these transgenic lines was verified by PCR amplification, GUS assays and Southern blot hybridization”
e1“cantaloupe cultivar Sun Lady”
Claim · model-written summary
The cantaloupe line containing pSA1175 remained sensitive to PRSV-W after inoculation.
- Taxon
- cantaloupeCucumis melo var. cantalupoe1
- Material
- pSA1175e0
- Treatment
- inoculation of these lines with PRSV-We0
- Interactor
- PRSV-We0pathogen
- Outcome
- remained sensitivee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro inoculation of these lines with PRSV-W revealed that whereas the line containing pSA1175 remained sensitive”
e1“cantaloupe cultivar Sun Lady”
Claim · model-written summary
Four cantaloupe lines containing pSA1304 were resistant to PRSV-W after inoculation.
- Taxon
- cantaloupeCucumis melo var. cantalupoe2
- Material
- pSA1304e0
- Treatment
- inoculation of these lines with PRSV-We1
- Interactor
- PRSV-We1pathogen
- Outcome
- resistante0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the four lines containing pSA1304 were resistant”
e1“In vitro inoculation of these lines with PRSV-W”
e2“cantaloupe cultivar Sun Lady”
Claim · model-written summary
Small RNA corresponding to the viral coat protein gene was present in resistant cantaloupe lines.
- Taxon
- cantaloupeCucumis melo var. cantalupoe1
- Interactor
- PRSV-We0pathogen
- Gene
- viral cp genee0
- Outcome
- small RNA species, presumably siRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“presence of small RNA species, presumably siRNA, corresponding to regions of the viral cp gene in transgenic lines resistant to PRSV-W”
e1“cantaloupe cultivar Sun Lady”
Claim · model-written summary
The authors infer post-transcriptional gene silencing contributes to PRSV-W resistance in cantaloupe.
- Taxon
- cantaloupeCucumis melo var. cantalupoe2
- Interactor
- PRSV-We1pathogen
- Outcome
- resistancee0
- Qualifier
- post-transcriptional gene silencinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“supports the involvement of post-transcriptional gene silencing in the establishment of resistance”
e1“resistant to PRSV-W”
e2“cantaloupe cultivar Sun Lady”
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