Abstract
The genome components of the Melon chlorotic leaf curl virus (MCLCuV) were cloned from symptomatic cantaloupe leaves collected in Guatemala during 2002. The MCLCuV DNA-A and DNA-B components shared their closest nucleotide identities among begomoviruses, at approximately 90 and 81%, respectively, with a papaya isolate of MCLCuV from Costa Rica. The closest relatives at the species level were other members of the Squash leaf curl virus (SLCV) clade, which is endemic in the southwestern United States and Mexico. Biolistic inoculation of cantaloupe seedlings with the MCLCuV DNA-A and -B components resulted in the development of characteristic disease symptoms, providing definitive evidence of causality. MCLCuV experimentally infected species within the Cucurbitaceae, Fabaceae, and Solanaceae. The potential for interspecific reassortment was examined for MCLCuV and its closest relatives, including the bean-restricted Bean calico mosaic virus (BCaMV), and three other cucurbit-infecting species, Cucurbit leaf crumple virus (CuLCrV), SLCV, and SMLCV. The cucurbit viruses have distinct but overlapping host ranges. All possible reassortants were established using heterologous combinations of the DNA-A or DNA-B components. Surprisingly, only certain reassortants arising from MCLCuV and BCaMV, or MCLCuV and CuLCrV, were viable in bean, even though it is a host of all of the "wild-type" (parent) viruses. The bean-restricted BCaMV was differentially assisted in systemically infecting the cucurbit test species by the components of the four cucurbit-adapted begomoviruses. In certain heterologous combinations, the BCaMV DNA-A or -B component was able to infect one or more cucurbit species. Generally, the reassortants were less virulent in the test hosts than the respective wild-type (parent) viruses, strongly implicating adaptive modulation of virulence. This is the first illustration of reassortment resulting in the host range expansion of a host-restricted begomovirus.
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Claims 14
Claim · model-written summary
MCLCuV genome components were cloned from symptomatic cantaloupe leaves.
- Taxon
- cantaloupe
Cucumis melo var. cantalupoe0 - Interactor
- Melon chlorotic leaf curl virusMelon chlorotic leaf curl viruse0
- Outcome
- genome componentse0
- Setting
- Guatemalae0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The genome components of the Melon chlorotic leaf curl virus (MCLCuV) were cloned from symptomatic cantaloupe leaves collected in Guatemala during 2002”
Claim · model-written summary
MCLCuV DNA-A shared approximately 90% nucleotide identity with a papaya isolate from Costa Rica.
- Taxon
- papaya
_-_DPLA_-_55200ec009622d7d42349f3583df310e.jpg?width=60)
Carica papayae0 - Interactor
- MCLCuVe0
- Outcome
- nucleotide identitiese0
- Value
- approximately 90e0
- Setting
- Costa Ricae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_DPLA_-_55200ec009622d7d42349f3583df310e.jpg?width=500)
Verbatim
e0“The MCLCuV DNA-A and DNA-B components shared their closest nucleotide identities among begomoviruses, at approximately 90 and 81%, respectively, with a papaya isolate of MCLCuV from Costa Rica”
Claim · model-written summary
MCLCuV DNA-B shared approximately 81% nucleotide identity with a papaya isolate from Costa Rica.
- Taxon
- papayaCarica papayae0
- Interactor
- MCLCuVe0
- Outcome
- nucleotide identitiese0
- Value
- 81%e0
- Setting
- Costa Ricae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The MCLCuV DNA-A and DNA-B components shared their closest nucleotide identities among begomoviruses, at approximately 90 and 81%, respectively, with a papaya isolate of MCLCuV from Costa Rica”
Claim · model-written summary
SLCV clade members were the closest relatives of MCLCuV at species level.
- Interactor
- Squash leaf curl virusSquash leaf curl viruse0
- Outcome
- closest relatives at the species levele0
Not reportedTaxon
Verbatim
e0“The closest relatives at the species level were other members of the Squash leaf curl virus (SLCV) clade”
Claim · model-written summary
Biolistic inoculation of cantaloupe seedlings with MCLCuV DNA-A and DNA-B caused characteristic disease symptoms.
- Taxon
- cantaloupeCucumis melo var. cantalupoe0
- Organ / stage
- seedlingse0
- Treatment
- Biolistic inoculatione0
- Interactor
- MCLCuVe0
- Outcome
- characteristic disease symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Biolistic inoculation of cantaloupe seedlings with the MCLCuV DNA-A and -B components resulted in the development of characteristic disease symptoms”
Claim · model-written summary
MCLCuV experimentally infected species in Cucurbitaceae, Fabaceae, and Solanaceae.
- Taxon
- Cucurbitaceae, Fabaceae, and SolanaceaeCucurbitaceaeFabaceaeSolanaceaee0
- Interactor
- MCLCuVe0
- Outcome
- experimentally infected speciese0
Not reportedDirection
Verbatim
e0“MCLCuV experimentally infected species within the Cucurbitaceae, Fabaceae, and Solanaceae”
Claim · model-written summary
Cucurbit viruses had distinct but overlapping host ranges.
Not reportedDirection
Verbatim
e0“The cucurbit viruses have distinct but overlapping host ranges”
Claim · model-written summary
All heterologous DNA-A and DNA-B combinations established reassortants.
- Outcome
- All possible reassortants were establishede0
Not reportedTaxonDirection
Verbatim
e0“All possible reassortants were established using heterologous combinations of the DNA-A or DNA-B components”
Claim · model-written summary
Only certain MCLCuV and BCaMV reassortants were viable in bean.
- Taxon
- beane0
- Interactor
- MCLCuVe0
- Second interactor
- BCaMVBean calico mosaic viruse0
- Outcome
- only certain reassortants arising from MCLCuV and BCaMVe0
Not reportedDirection
Verbatim
e0“only certain reassortants arising from MCLCuV and BCaMV, or MCLCuV and CuLCrV, were viable in bean”
Claim · model-written summary
Only certain MCLCuV and CuLCrV reassortants were viable in bean.
- Taxon
- beane0
- Interactor
- MCLCuVe0
- Second interactor
- CuLCrVe0
- Outcome
- MCLCuV and CuLCrV, were viable in beane0
Not reportedDirection
Verbatim
e0“only certain reassortants arising from MCLCuV and BCaMV, or MCLCuV and CuLCrV, were viable in bean”
Claim · model-written summary
Components of four cucurbit-adapted begomoviruses differentially assisted systemic BCaMV infection of cucurbit test species.
- Taxon
- cucurbite0
- Treatment
- components of the four cucurbit-adapted begomovirusese0
- Interactor
- BCaMVBean calico mosaic viruse0
- Outcome
- systemically infecting the cucurbit test speciese0
Not reportedComparatorDirection
Verbatim
e0“The bean-restricted BCaMV was differentially assisted in systemically infecting the cucurbit test species by the components of the four cucurbit-adapted begomoviruses”
Claim · model-written summary
In some heterologous combinations, BCaMV DNA-A or DNA-B infected at least one cucurbit species.
- Taxon
- cucurbite0
- Interactor
- BCaMVBean calico mosaic viruse0
- Outcome
- able to infect one or more cucurbit speciese0
Not reportedDirection
Verbatim
e0“In certain heterologous combinations, the BCaMV DNA-A or -B component was able to infect one or more cucurbit species”
Claim · model-written summary
Reassortants were generally less virulent in test hosts than their parent viruses.
Verbatim
e0“Generally, the reassortants were less virulent in the test hosts than the respective wild-type (parent) viruses”
Claim · model-written summary
Reassortment expanded host range of the host-restricted begomovirus.
Not reportedTaxon
Verbatim
e0“reassortment resulting in the host range expansion of a host-restricted begomovirus”
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