Abstract
Experiments comparing the photosynthetic responses of a chilling-resistant species (Pisum sativum L. cv Alaska) and a chilling-sensitive species (Cucumis sativus L. cv Ashley) have shown that cucumber photosynthesis is adversely affected by chilling temperatures in the light, while pea photosynthesis is not inhibited by chilling in the light. To further investigate the site of the differential response of these two species to chilling stress, thylakoid membranes were isolated under various conditions and rates of photosynthetic electron transfer were determined. Preliminary experiments revealed that the integrity of cucumber thylakoids from 25 degrees C-grown plants was affected by the isolation temperature; cucumber thylakoids isolated at 5 degrees C in 400 millimolar NaCl were uncoupled, while thylakoids isolated at room temperature in 400 millimolar NaCl were coupled, as determined by addition of gramicidin. The concentration of NaCl in the homogenization buffer was found to be a critical factor in the uncoupling of cucumber thylakoids at 5 degrees C. In contrast, pea thylakoid membranes were not influenced by isolation temperatures or NaCl concentrations. In a second set of experiments, thylakoid membranes were isolated from pea and cucumber plants at successive intervals during a whole-plant light period chilling stress (5 degrees C). During wholeplant chilling, thylakoids isolated from cucumber plants chilled in the light were uncoupled even when the membranes were isolated at warm temperatures. Pea thylakoids were not uncoupled by the whole-plant chilling treatment. The difference in integrity of thylakoid membrane coupling following chilling in the light demonstrates a fundamental difference in photosynthetic function between these two species that may have some bearing on why pea is a chilling-resistant plant and cucumber is a chilling-sensitive plant.
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Claims 8
Claim · model-written summary
Chilling in the light adversely affected cucumber photosynthesis.
- Taxon
- cucumber
Cucumis sativuse0 - Treatment
- chilling temperatures in the lighte0
- Outcome
- photosynthesise0
- Direction
- adversely affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cucumber photosynthesis is adversely affected by chilling temperatures in the light”
Claim · model-written summary
Chilling in the light did not inhibit pea photosynthesis.
- Taxon
- pea
Lathyrus oleraceuse0 - Treatment
- chilling in the lighte0
- Outcome
- photosynthesise0
- Direction
- not inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea photosynthesis is not inhibited by chilling in the light”
Claim · model-written summary
Cucumber thylakoids isolated at 5 degrees C in 400 millimolar NaCl were uncoupled.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- 5 degrees C in 400 millimolar NaCle0
- Outcome
- thylakoidse0
- Qualifier
- uncouplede0
- Comparator
- room temperature in 400 millimolar NaCle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cucumber thylakoids isolated at 5 degrees C in 400 millimolar NaCl were uncoupled, while thylakoids isolated at room temperature in 400 millimolar NaCl were coupled”
Claim · model-written summary
Cucumber thylakoids isolated at room temperature in 400 millimolar NaCl were coupled.
- Taxon
- CucumberCucumis sativust0
- Outcome
- thylakoids isolated at room temperature in 400 millimolar NaCl were couplede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thylakoids isolated at room temperature in 400 millimolar NaCl were coupled”
t0“Cucumber”
Claim · model-written summary
NaCl concentration in homogenization buffer was critical for cucumber thylakoid uncoupling at 5 degrees C.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- concentration of NaCl in the homogenization buffere0
- Outcome
- uncoupling of cucumber thylakoidse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The concentration of NaCl in the homogenization buffer was found to be a critical factor in the uncoupling of cucumber thylakoids at 5 degrees C.”
Claim · model-written summary
Pea thylakoid membranes were unaffected by isolation temperature or NaCl concentration.
- Taxon
- peaLathyrus oleraceuse0
- Treatment
- isolation temperatures or NaCl concentrationse0
- Outcome
- thylakoid membranese0
- Direction
- not influencede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pea thylakoid membranes were not influenced by isolation temperatures or NaCl concentrations.”
Claim · model-written summary
Whole-plant chilling in the light uncoupled cucumber thylakoids even with warm membrane isolation.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- chilled in the lighte0
- Outcome
- thylakoidse0
- Qualifier
- uncoupled even when the membranes were isolated at warm temperaturese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thylakoids isolated from cucumber plants chilled in the light were uncoupled even when the membranes were isolated at warm temperatures.”
Claim · model-written summary
Whole-plant chilling did not uncouple pea thylakoids.
- Taxon
- PeaLathyrus oleraceuse0
- Treatment
- whole-plant chilling treatmente0
- Outcome
- thylakoidse0
- Direction
- not uncouplede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pea thylakoids were not uncoupled by the whole-plant chilling treatment.”
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