Abstract
Treatment of etiolated and excisedCucurbita cotyledons with exogenous cytokinin (benzyladenine) in darkness or light results in a marked stimulation of Rubisco activity, content of enzyme protein, and incorporation of labelled precursors into it, indicating cytokinin-stimulatedde novo synthesis of the enzyme. Cell-free translations of RNA in the wheat germ andE. coli systems show an increase in both large and small subunit mRNA amounts relative to the increase of total RNA under the influence of the phytohormone and light. This increase in the level of translatable RNA is confirmed by RNA hybridization with the Rubisco large subunit gene of spinach. In addition, our results demonstrating additive effects of benzyladenine and light in cotyledon and chloroplast development suggest that the two factors co-act independently in the causal sequence of Rubisco gene expression. The data are discussed in a general view of cytokinin action in gene expression steps.
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Claims 7
Claim · model-written summary
Benzyladenine stimulated Rubisco activity in excised Cucurbita cotyledons in darkness or light.
- Taxon
- pumpkint0
- Organ / stage
- cotyledonse0
- Treatment
- exogenous cytokinin (benzyladenine)e0
- Outcome
- Rubisco activitye0
- Direction
- stimulatione0
Not reportedComparator
Verbatim
e0“Treatment of etiolated and excisedCucurbita cotyledons with exogenous cytokinin (benzyladenine) in darkness or light results in a marked stimulation of Rubisco activity”
t0“pumpkin cotyledons”
Claim · model-written summary
Benzyladenine stimulated Rubisco enzyme protein content.
- Taxon
- pumpkint0
- Organ / stage
- cotyledonse0
- Treatment
- exogenous cytokinin (benzyladenine)e0
- Outcome
- content of enzyme proteine0
- Direction
- stimulatione0
Not reportedComparator
Verbatim
e0“Treatment of etiolated and excisedCucurbita cotyledons with exogenous cytokinin (benzyladenine) in darkness or light results in a marked stimulation of Rubisco activity, content of enzyme protein”
t0“pumpkin cotyledons”
Claim · model-written summary
Benzyladenine stimulated incorporation of labelled precursors into Rubisco.
Not reportedTreatmentComparator
Verbatim
e0“marked stimulation of Rubisco activity, content of enzyme protein, and incorporation of labelled precursors into it”
t0“pumpkin cotyledons”
Claim · model-written summary
Cytokinin and light increased large and small subunit mRNA relative to total RNA.
Verbatim
e0“Cell-free translations of RNA in the wheat germ andE. coli systems show an increase in both large and small subunit mRNA amounts relative to the increase of total RNA under the influence of the phytohormone and light”
t0“pumpkin cotyledons”
Claim · model-written summary
RNA hybridization confirmed increased translatable RNA.
Not reportedComparatorValue
Verbatim
e0“This increase in the level of translatable RNA is confirmed by RNA hybridization with the Rubisco large subunit gene of spinach”
t0“pumpkin cotyledons”
Claim · model-written summary
Benzyladenine and light had additive effects on cotyledon and chloroplast development.
- Taxon
- pumpkint0
- Treatment
- benzyladenine and lighte0
- Outcome
- cotyledon and chloroplast developmente0
- Direction
- additive effectse0
Not reportedComparator
Verbatim
e0“our results demonstrating additive effects of benzyladenine and light in cotyledon and chloroplast development”
t0“pumpkin cotyledons”
Claim · model-written summary
The authors infer that benzyladenine and light independently co-act in Rubisco gene expression.
Verbatim
e0“suggest that the two factors co-act independently in the causal sequence of Rubisco gene expression”
t0“pumpkin cotyledons”
The same questions in other papers
N-benzyladenine · physiology: enzyme activity · Cucurbita (pumpkins)Results agree
2 papers in the same direction as this paper
- positive
- positive
N-benzyladenine · growth · Cucurbita (pumpkins)Results agree
4 papers in the same direction as this paper
- positive
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1 paper more in this group
See all 5 papers →light · growth · Cucurbita (pumpkins)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- negative
- no effect
- negative
2 papers more in this group
See all 6 papers →class · molecular: expression · Cucurbita (pumpkins)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.