Abstract
Plant regeneration from tissue cultures of summer squash (Cucurbita pepo L. ev. YC60) has been observed. Embryogenic callus tissues were initiated when cotyledons of mature seeds were excised and cultured on Murashige and Skoog (MS) medium supplemented with either 22.7 μ m 2,4-D or a combination of 4.7 μ m 2,4,5-T, 4 μ m BA, and 0.5 μ m kinetin. Clusters of somatic embryos were found in callus tissue. Maturation of these somatic embryos was effected by transfer of embryogenic callus tissues to MS supplemented with 0.5 μ m NAA and 0.25 μ m kinetin. Regenerated mature plants were morphologically normal and set fruits containing seeds that germinated normally. Chemical names used: 6-benzylaminopurine (BA); 2,4-dichlorophenoxyacetic acid (2,4-D); α - napthaleneacetic acid (NAA); 2,4,5-trichlorophenoxyacetic acid (2,4,5-T).
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Claims 7
Claim · model-written summary
Summer squash plants regenerated from tissue cultures.
Verbatim
e0“Plant regeneration from tissue cultures of summer squash (Cucurbita pepo L. ev. YC60) has been observed.”
Claim · model-written summary
A culture medium supplemented with 2,4-D initiated embryogenic callus from mature seed cotyledons.
- Taxon
- summer squashe1
- Material
- YC60e1
- Organ / stage
- cotyledons of mature seedse0
- Treatment
- 22.7 μ m 2,4-De0
- Outcome
- Embryogenic callus tissues were initiatede0
Not reportedComparatorDirection
Verbatim
e0“Embryogenic callus tissues were initiated when cotyledons of mature seeds were excised and cultured on Murashige and Skoog (MS) medium supplemented with either 22.7 μ m 2,4-D or a combination of 4.7 μ m 2,4,5-T, 4 μ m BA, and 0.5 μ m kinetin.”
e1“summer squash (Cucurbita pepo L. ev. YC60)”
Claim · model-written summary
A combination of 2,4,5-T, BA, and kinetin also initiated embryogenic callus from mature seed cotyledons.
- Taxon
- summer squashe1
- Material
- YC60e1
- Organ / stage
- cotyledons of mature seedse0
- Treatment
- 4.7 μ m 2,4,5-T, 4 μ m BA, and 0.5 μ m kinetine0
- Outcome
- Embryogenic callus tissues were initiatede0
Not reportedComparatorDirection
Verbatim
e0“Embryogenic callus tissues were initiated when cotyledons of mature seeds were excised and cultured on Murashige and Skoog (MS) medium supplemented with either 22.7 μ m 2,4-D or a combination of 4.7 μ m 2,4,5-T, 4 μ m BA, and 0.5 μ m kinetin.”
e1“summer squash (Cucurbita pepo L. ev. YC60)”
Claim · model-written summary
Somatic embryo clusters developed in squash callus tissue.
Verbatim
e0“Clusters of somatic embryos were found in callus tissue.”
e1“summer squash (Cucurbita pepo L. ev. YC60)”
Claim · model-written summary
Transfer to medium with NAA and kinetin matured squash somatic embryos.
- Taxon
- summer squashe1
- Material
- YC60e1
- Treatment
- MS supplemented with 0.5 μ m NAA and 0.25 μ m kinetine0
- Outcome
- Maturation of these somatic embryose0
Not reportedComparatorDirection
Verbatim
e0“Maturation of these somatic embryos was effected by transfer of embryogenic callus tissues to MS supplemented with 0.5 μ m NAA and 0.25 μ m kinetin.”
e1“summer squash (Cucurbita pepo L. ev. YC60)”
Claim · model-written summary
Regenerated squash plants were morphologically normal.
Verbatim
e0“Regenerated mature plants were morphologically normal and set fruits containing seeds that germinated normally.”
e1“summer squash (Cucurbita pepo L. ev. YC60)”
Claim · model-written summary
Regenerated squash plants set fruit with seeds that germinated normally.
Verbatim
e0“Regenerated mature plants were morphologically normal and set fruits containing seeds that germinated normally.”
e1“summer squash (Cucurbita pepo L. ev. YC60)”
The same questions in other papers
2,4-D · growth · Cucurbita (pumpkins)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
1-naphthaleneacetic acid · growth · Cucurbita (pumpkins)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
N-benzyladenine · growth · Cucurbita (pumpkins)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.