Abstract
The aim of this work was to evaluate the properties and gene expression of isoforms of β-galactosidase, isolated from the stems of seedlings of the cowpea cv. Pitiuba, at different stages of development and sown in the absence and presence of NaCl. Seeds were sown onto sheets of filter paper soaked in distilled water (control) and a 100 mM NaCl solution. The plants were collected at the III (ED III) and VIII (ED VIII) stages of development. Isoforms of the enzyme β-gal I, II and III presented molecular weights of 89, 146 and 124 kDa respectively, and the same pH (4.0) and optimum test temperature (55 °C) under all the conditions studied. The three isoforms were stable up to 50 °C. The thermal inactivation curves of the enzymes as a function of time were biphasic at a temperature of 60 °C. Analysis of the gene expression of β-galactosidase was carried out using degenerate primers and total RNA from the stems of seedlings at ED III and ED VIII, sown both as control and under saline treatments. The results showed higher transcript levels at ED VIII, with reduced levels of β-galactosidase transcripts under saline treatment, and that β-gal I, II and III do not undergo changes during the early stages of development. There are a few differences in the properties of the three isoforms. Salinity affected the gene expression of enzymes. The enzymes were expressed differently for ED III and ED VIII.
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Claims 8
Claim · model-written summary
β-gal I had molecular weight 89.
- Taxon
- cowpea
Vigna unguiculatat0 - Gene
- β-gal Ie0
- Outcome
- molecular weightse0
- Value
- 89e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isoforms of the enzyme β-gal I, II and III presented molecular weights of 89, 146 and 124 kDa respectively, and the same pH (4.0) and optimum test temperature (55 °C) under all the conditions studied”
t0“cowpea”
Claim · model-written summary
II had molecular weight 146.
- Taxon
- cowpeaVigna unguiculatat0
- Gene
- IIe0
- Outcome
- molecular weightse0
- Value
- 146e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isoforms of the enzyme β-gal I, II and III presented molecular weights of 89, 146 and 124 kDa respectively, and the same pH (4.0) and optimum test temperature (55 °C) under all the conditions studied”
t0“cowpea”
Claim · model-written summary
III had molecular weight 124 kDa.
- Taxon
- cowpeaVigna unguiculatat0
- Gene
- IIIe0
- Outcome
- molecular weightse0
- Value
- 124 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isoforms of the enzyme β-gal I, II and III presented molecular weights of 89, 146 and 124 kDa respectively, and the same pH (4.0) and optimum test temperature (55 °C) under all the conditions studied”
t0“cowpea”
Claim · model-written summary
All three isoforms shared pH 4.0 and 55 °C temperature optima.
- Taxon
- cowpeaVigna unguiculatat0
- Gene
- β-gal I, II and IIIe0
- Outcome
- same pH (4.0) and optimum test temperature (55 °C)e0
- Value
- 4.0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isoforms of the enzyme β-gal I, II and III presented molecular weights of 89, 146 and 124 kDa respectively, and the same pH (4.0) and optimum test temperature (55 °C) under all the conditions studied”
t0“cowpea”
Claim · model-written summary
All three isoforms were stable to 50 °C.
- Taxon
- cowpeaVigna unguiculatat0
- Outcome
- stable up to 50 °Ce0
- Value
- 50 °Ce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The three isoforms were stable up to 50 °C”
t0“cowpea”
Claim · model-written summary
β-galactosidase transcripts were higher at ED VIII.
- Taxon
- cowpeaVigna unguiculatat0
- Organ / stage
- ED VIIIe0
- Gene
- β-galactosidasee0
- Outcome
- transcript levelse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the gene expression of β-galactosidase was carried out using degenerate primers and total RNA from the stems of seedlings at ED III and ED VIII, sown both as control and under saline treatments. The results showed higher transcript levels at ED VIII”
t0“cowpea”
Claim · model-written summary
Salinity reduced β-galactosidase transcripts.
- Taxon
- cowpeaVigna unguiculatat0
- Treatment
- saline treatmente0
- Gene
- β-galactosidasee0
- Outcome
- β-galactosidase transcriptse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with reduced levels of β-galactosidase transcripts under saline treatment”
t0“cowpea”
Claim · model-written summary
β-gal I, II and III did not change during early development.
- Taxon
- cowpeaVigna unguiculatat0
- Gene
- β-gal I, II and IIIe0
- Outcome
- changes during the early stages of developmente0
- Direction
- do not undergo changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“β-gal I, II and III do not undergo changes during the early stages of development”
t0“cowpea”
The same questions in other papers
salinity · molecular: expression · Vigna unguiculata (cowpea)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.