Abstract
In order to clarify the effect of abscisic acid (ABA) on anaerobic tolerance in alfalfa ( Medicago sativa L.), the seedlings were subjected to anaerobic stress after pretreatment with ABA. At concentrations> 1 μ M , ABA pretreatment increased the root viability of the seedlings to anaerobic stress and the viability increased with increasing ABA doses. At 100 μ M ABA, the viability was 2.5‐fold greater as compared with that of control seedlings. Roots of the seedlings rapidly lost ATP under the anaerobic stress; however, the decrease in ATP was much slower in the ABA‐pretreated seedlings than the control seedlings. At 12 h after onset of the stress, ATP concentrations in the roots of 100 and 10 μ M ABA‐pretreated seedlings were 2.7‐ and 2.0‐fold that of the control seedlings, respectively. During the period of ABA pretreatment under aerobic condition, ABA increased alcohol dehydrogenase (ADH, EC 1.1.1.1) activity in the roots until 12 h and then leveled off. The maximum ADH activities were 4.3‐ and 2.8‐fold that in the roots of the control seedlings for 100 and 10 μ M ABA‐pretreated seedlings, respectively. After being subjected to the anaerobic stress, both ADH activities in the roots of the ABA‐pretreated and the control seedlings increased but the differences in their activity remained. These results suggest that ABA pretreatment may maintain ATP level due to induction of ADH activity, which may be one of the causes of increasing anaerobic tolerance in the seedlings.
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Claims 11
Claim · model-written summary
ABA above 1 μ M increased alfalfa root viability under anaerobic stress, with a dose response.
- Taxon
- alfalfa
Medicago sativat0 - Treatment
- ABA pretreatmente0
- Outcome
- root viabilitye0
- Direction
- increasede0
- Qualifier
- concentrations> 1 μ Me0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At concentrations> 1 μ M , ABA pretreatment increased the root viability of the seedlings to anaerobic stress and the viability increased with increasing ABA doses”
t0“alfalfa seedlings”
Claim · model-written summary
At 100 μ M ABA, root viability was 2.5-fold that of controls.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- 100 μ M ABAe0
- Outcome
- viabilitye0
- Direction
- greatere0
- Value
- 2.5‐folde0
- Comparator
- control seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At 100 μ M ABA, the viability was 2.5‐fold greater as compared with that of control seedlings”
t0“alfalfa seedlings”
Claim · model-written summary
Alfalfa seedling roots rapidly lost ATP during anaerobic stress.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- Rootse0
- Treatment
- anaerobic stresse0
- Outcome
- ATPe0
- Direction
- loste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Roots of the seedlings rapidly lost ATP under the anaerobic stress”
t0“alfalfa seedlings”
Claim · model-written summary
ABA pretreatment slowed the ATP decrease relative to controls.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- ABA‐pretreated seedlingse0
- Outcome
- decrease in ATPe0
- Direction
- much slowere0
- Comparator
- control seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the decrease in ATP was much slower in the ABA‐pretreated seedlings than the control seedlings”
t0“alfalfa seedlings”
Claim · model-written summary
At 12 h, 100 μ M ABA produced root ATP at 2.7-fold control levels.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- 100e0
- Outcome
- ATP concentrations in the rootse0
- Value
- 2.7‐e0
- Qualifier
- At 12 h after onset of the stresse0
- Comparator
- control seedlingse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At 12 h after onset of the stress, ATP concentrations in the roots of 100 and 10 μ M ABA‐pretreated seedlings were 2.7‐ and 2.0‐fold that of the control seedlings, respectively”
t0“alfalfa seedlings”
Claim · model-written summary
At 12 h, 10 μ M ABA produced root ATP at 2.0-fold control levels.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- 10 μ M ABA‐pretreated seedlingse0
- Outcome
- ATP concentrations in the rootse0
- Value
- 2.0‐folde0
- Comparator
- control seedlingse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At 12 h after onset of the stress, ATP concentrations in the roots of 100 and 10 μ M ABA‐pretreated seedlings were 2.7‐ and 2.0‐fold that of the control seedlings, respectively”
t0“alfalfa seedlings”
Claim · model-written summary
ABA raised root ADH activity during aerobic pretreatment until 12 h, then activity leveled off.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- rootse0
- Treatment
- ABAe0
- Outcome
- alcohol dehydrogenase (ADH, EC 1.1.1.1) activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA increased alcohol dehydrogenase (ADH, EC 1.1.1.1) activity in the roots until 12 h and then leveled off”
t0“alfalfa seedlings”
Claim · model-written summary
Maximum root ADH activity with 100 μ M ABA was 4.3-fold control.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- 100e0
- Outcome
- ADH activitiese0
- Direction
- maximume0
- Value
- 4.3‐e0
- Comparator
- control seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum ADH activities were 4.3‐ and 2.8‐fold that in the roots of the control seedlings for 100 and 10 μ M ABA‐pretreated seedlings, respectively”
t0“alfalfa seedlings”
Claim · model-written summary
Maximum root ADH activity with 10 μ M ABA was 2.8-fold control.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- 10 μ M ABA‐pretreated seedlingse0
- Outcome
- ADH activitiese0
- Direction
- maximume0
- Value
- 2.8‐folde0
- Comparator
- control seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The maximum ADH activities were 4.3‐ and 2.8‐fold that in the roots of the control seedlings for 100 and 10 μ M ABA‐pretreated seedlings, respectively”
t0“alfalfa seedlings”
Claim · model-written summary
Anaerobic stress increased root ADH activity in both ABA-pretreated and control seedlings while preserving their difference.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- ADH activities in the rootse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both ADH activities in the roots of the ABA‐pretreated and the control seedlings increased but the differences in their activity remained”
t0“alfalfa seedlings”
Claim · model-written summary
ABA pretreatment may maintain ATP through induction of ADH activity and thereby increase anaerobic tolerance.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- ABA pretreatmente0
- Outcome
- anaerobic tolerancee0
- Qualifier
- induction of ADH activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA pretreatment may maintain ATP level due to induction of ADH activity, which may be one of the causes of increasing anaerobic tolerance in the seedlings”
t0“alfalfa seedlings”
The same questions in other papers
(+)-abscisic acid · growth · Medicago sativa (alfalfa)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.