Abstract
A technique has been developed which allows the culture of barley cv. Igri anthers in isolated spikes and spikelets through meiosis until the mid-late uninucleate microspore stage. Light microscopical analyses and measurements of the duration of the individual substages of meiosis and microsporogenesis revealed a developmental sequence indistinguishable from that observed in vivo. Embryogenesis was induced in microspores at the mid-late uninucleate stage, when anthers from cultured spikes and spikelets were plated on induction medium and green plants were regenerated from the embryoids. Among different media tested for their ability to sustain a normal development of the microsporocytes and the microspores, modified Murashige and Skoog (MS) and Kao media solidified with agarose yielded optimal growth of the isolated spikes and spikelets. On a modified MS medium with 100 g/1 maltose and 1 mg/1 6-benzylaminopurine (BAP) 62 % of the anthers survived. Fifty-one percent of these anthers responded with microspore embryogenesis and a mean of 2.4 green plants were regenerated per responding anther. The albino to green plant ratio was 0.5. The significance of this culture system for establishing a transformation system with gene targeting mediated by homologous meiotic recombination is discussed.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Cultured anthers showed a developmental sequence indistinguishable from in vivo development.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree1 - Material
- cv. Igrie1
- Outcome
- a developmental sequence indistinguishable from that observed in vivoe0
- Comparator
- in vivoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Light microscopical analyses and measurements of the duration of the individual substages of meiosis and microsporogenesis revealed a developmental sequence indistinguishable from that observed in vivo”
e1“barley cv. Igri anthers”
Claim · model-written summary
Plating mid-late uninucleate anthers induced microspore embryogenesis and regenerated green plants.
- Taxon
- barleyHordeum vulgaree1
- Material
- cv. Igrie1
- Organ / stage
- mid-late uninucleate stagee0
- Treatment
- plated on induction mediume0
- Outcome
- Embryogenesise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenesis was induced in microspores at the mid-late uninucleate stage, when anthers from cultured spikes and spikelets were plated on induction medium and green plants were regenerated from the embryoids”
e1“barley cv. Igri anthers”
Claim · model-written summary
Modified MS and Kao media solidified with agarose yielded optimal growth of isolated spikes and spikelets.
- Taxon
- barleyHordeum vulgaree1
- Material
- cv. Igrie1
- Treatment
- modified Murashige and Skoog (MS) and Kao media solidified with agarosee0
- Outcome
- growth of the isolated spikes and spikeletse0
- Direction
- optimale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“modified Murashige and Skoog (MS) and Kao media solidified with agarose yielded optimal growth of the isolated spikes and spikelets”
e1“barley cv. Igri anthers”
Claim · model-written summary
On modified MS medium, 62 % of anthers survived.
- Taxon
- barleyHordeum vulgaree1
- Material
- cv. Igrie1
- Treatment
- modified MS medium with 100 g/1 maltose and 1 mg/1 6-benzylaminopurine (BAP)e0
- Outcome
- anthers survivede0
- Value
- 62 %e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On a modified MS medium with 100 g/1 maltose and 1 mg/1 6-benzylaminopurine (BAP) 62 % of the anthers survived”
e1“barley cv. Igri anthers”
Claim · model-written summary
Fifty-one percent of surviving anthers responded with microspore embryogenesis.
- Taxon
- barleyHordeum vulgaree1
- Material
- cv. Igrie1
- Outcome
- anthers responded with microspore embryogenesise0
- Value
- Fifty-one percente0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fifty-one percent of these anthers responded with microspore embryogenesis”
e1“barley cv. Igri anthers”
Claim · model-written summary
Responding anthers regenerated a mean of 2.4 green plants.
- Taxon
- barleyHordeum vulgaree1
- Material
- cv. Igrie1
- Outcome
- green plants were regenerated per responding anthere0
- Value
- 2.4e0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a mean of 2.4 green plants were regenerated per responding anther”
e1“barley cv. Igri anthers”
Claim · model-written summary
The albino to green plant ratio was 0.5.
- Taxon
- barleyHordeum vulgaree1
- Material
- cv. Igrie1
- Outcome
- albino to green plant ratioe0
- Value
- 0.5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The albino to green plant ratio was 0.5”
e1“barley cv. Igri anthers”
The same questions in other papers
N-benzyladenine · survival · Hordeum vulgare (barley)Positive and negative results
1 in the opposite direction
- no effect
in vitro · yield · Hordeum vulgare (barley)Positive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
6 papers more in this group
See all 10 papers →in vitro · survival · Hordeum vulgare (barley)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.