Abstract
BACKGROUND: The overall research objective was to develop single cell plant cultures as a model system to facilitate functional genomics of monocots, in particular wheat and barley. The essential first step towards achieving the stated objective was the development of a robust, viable single cell suspension culture from both species. RESULTS: We established growth conditions to allow routine culturing of somatic cells in 24 well microtiter plate format. Evaluation of the wheat and barley cell suspension as model cell system is a multi step process. As an initial step in the evaluation procedure we chose to study the impact of selected abiotic stress elicitors at the physiological, biochemical and molecular level. We report the results of osmotic stress imposed by NaCl and PEG. As proline is an important osmoprotectant of the cereal cells, colorimetric assay for proline detection was developed for small volumes (200 μl). We performed RT-PCR experiments to study the change in the expression of the genes encoding Δ1-pyrroline-5-carboxylate synthetase (P5CS) and Δ1-pyrroline-5-carboxylate reductase (PC5R) in response to abiotic stress. CONCLUSIONS: We found differences between the wheat and barley suspension cultures, barley being more tolerant to the applied osmotic stresses. We suggested a model to explain the obtained differences in stress tolerance between the two species. The suspension cell cultures have proven useful for determining changes in proline concentration and expression level of genes (P5CS, P5CR) under various treatments and we suggest that the cells can be used as a model host system to study gene expression and regulation in monocots.
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Claims 2
Claim · model-written summary
Barley suspension cells were more tolerant than wheat cells to osmotic stress imposed by NaCl and PEG.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- osmotic stress imposed by NaCl and PEGe1
- Outcome
- more tolerant to the applied osmotic stressese0
- Direction
- more tolerante0
- Comparator
- wheate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“single cell suspension from wheat and barley”
e0“We found differences between the wheat and barley suspension cultures, barley being more tolerant to the applied osmotic stresses”
e1“We report the results of osmotic stress imposed by NaCl and PEG”
Claim · model-written summary
The cells can be used as a model host system to study gene expression and regulation in monocots.
- Taxon
- wheat and barleyHordeum vulgare
Triticum aestivumt0 - Outcome
- model host systeme2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“single cell suspension from wheat and barley”
e2“we suggest that the cells can be used as a model host system to study gene expression and regulation in monocots”
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