Abstract
BACKGROUND: Previous studies showed the ability of Pseudomonas putida strain BTP1 to promote induced systemic resistance (ISR) in different host plants. Since ISR is long-lasting and not conducive for development of resistance of the targeted pathogen, this phenomenon can take part of disease control strategies. However, in spite of the numerous examples of ISR induced by PGPR in plants, only a few biochemical studies have associated the protective effect with specific host metabolic changes. RESULTS: In this study, we showed the protective effect of this bacterium in tomato against Botrytis cinerea. Following treatment by P. putida BTP1, analyses of acid-hydrolyzed leaf extracts showed an accumulation of antifungal material after pathogen infection. The fungitoxic compounds thus mainly accumulate as conjugates from which active aglycones may be liberated through the activity of hydrolytic enzymes. These results suggest that strain BTP1 can elicit systemic phytoalexin accumulation in tomato as one defence mechanism. On another hand, we have shown that key enzymes of the lipoxygenase pathway are stimulated in plants treated with the bacteria as compared with control plants. Interestingly, this stimulation is observed only after pathogen challenge in agreement with the priming concept almost invariably associated with the ISR phenomenon. CONCLUSION: Through the demonstration of phytoalexin accumulation and LOX pathway stimulation in tomato, this work provides new insights into the diversity of defence mechanisms that are inducible by non-pathogenic bacteria in the context of ISR.
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Claims 5
Claim · model-written summary
Pseudomonas putida BTP1 protected tomato against Botrytis cinerea.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- Pseudomonas putidastrain BTP1t0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Second interactor
- Pseudomonas putidastrain BTP1t0biocontrol_agent
- Outcome
- protective effecte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we showed the protective effect of this bacterium in tomato against Botrytis cinerea.”
t0“Pseudomonas putidastrain BTP1”
Claim · model-written summary
P. putida BTP1 treatment was followed by antifungal material accumulation after infection.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- leafe0
- Treatment
- treatment by P. putida BTP1e0
- Interactor
- P. putida BTP1
Pseudomonas putidae0biocontrol_agent - Outcome
- antifungal materiale0
- Direction
- accumulatione0
- Qualifier
- after pathogen infectione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Following treatment by P. putida BTP1, analyses of acid-hydrolyzed leaf extracts showed an accumulation of antifungal material after pathogen infection.”
t0“tomato”
Claim · model-written summary
Fungitoxic compounds accumulated mainly as conjugates.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- fungitoxic compoundse0
- Direction
- accumulatee0
- Qualifier
- mainly accumulate as conjugatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fungitoxic compounds thus mainly accumulate as conjugates from which active aglycones may be liberated through the activity of hydrolytic enzymes.”
t0“tomato”
Claim · model-written summary
BTP1 treatment stimulated key lipoxygenase pathway enzymes compared with controls.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- treated with the bacteriae0
- Interactor
- Pseudomonas putidastrain BTP1t0biocontrol_agent
- Gene
- key enzymes of the lipoxygenase pathwaye0
- Outcome
- key enzymes of the lipoxygenase pathwaye0
- Direction
- stimulatede0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“key enzymes of the lipoxygenase pathway are stimulated in plants treated with the bacteria as compared with control plants.”
t0“Systemic resistance and lipoxygenase-related defence response induced in tomato by Pseudomonas putidastrain BTP1”
Claim · model-written summary
Lipoxygenase pathway stimulation occurred only after pathogen challenge.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- stimulatione0
- Qualifier
- only after pathogen challengee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this stimulation is observed only after pathogen challenge”
t0“tomato”
The same questions in other papers
Pseudomonas putida · molecular: metabolites · Solanum lycopersicum (tomato)Results agree
1 paper in the same direction as this paper
- positive
inoculation · physiology: enzyme activity · Solanum lycopersicum (tomato)Positive and negative results
13 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
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See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.