Abstract
Abstract Nutrient requirements of callus tissues previously obtained from Vigna sinensis hypocotyl segments were investigated on modified White's (Miller and Skoog) and Murashige and Skoog's media containing 1 mg/1 of each of 2,4‐D and kinetin, 2% sucrose and 1% agar. Yields of tissues on the latter medium were 3 times more than the former. After studying various organic and inorganic nutrients, two synthetic media have been developed for callus cultures. The above modified White's medium further supplemented with 2% sucrose, and 500 mg/1 of urea or asparagine was suitable for a compact callus with a dry‐to‐fresh‐weight ratio around 8% and on this the tissues grew 15‐ to 17‐fold in 5 weeks. Similarly the Murashige and Skoog's medium further enriched with 2% sucrose, 400 mg/1 of urea or a mixture of aspartic acid (750 mg/1), glutamic acid (250 mg/1), asparagine (250 mg/1) and arginine (250 mg/1), was apt for a friable callus with a dry to fresh weight ratio of 3 to 4% and it promoted a 45‐ to 50‐fold increase in growth in 5 weeks.
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Claims 5
Claim · model-written summary
Murashige and Skoog medium produced three times the callus tissue yield of modified White medium.
- Taxon
- Vigna sinensis
Vigna unguiculata subsp. unguiculatat0 - Treatment
- Murashige and Skoog's mediae1
- Outcome
- Yields of tissuese0
- Direction
- moree0
- Value
- 3 timese0
- Comparator
- modified White's (Miller and Skoog)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Yields of tissues on the latter medium were 3 times more than the former.”
e1“modified White's (Miller and Skoog) and Murashige and Skoog's media”
t0“Vigna sinensis”
Claim · model-written summary
Supplemented modified White medium supported compact Vigna callus with an approximately 8% dry-to-fresh-weight ratio.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- modified White's mediume0
- Outcome
- compact calluse0
- Value
- 8%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The above modified White's medium further supplemented with 2% sucrose, and 500 mg/1 of urea or asparagine was suitable for a compact callus with a dry‐to‐fresh‐weight ratio around 8% and on this the tissues grew 15‐ to 17‐fold in 5 weeks.”
t0“Vigna sinensis”
Claim · model-written summary
Vigna callus grew 15‐ to 17‐fold in five weeks on supplemented modified White medium.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- modified White's mediume0
- Outcome
- tissues grewe0
- Direction
- grewe0
- Value
- 15‐ to 17‐folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The above modified White's medium further supplemented with 2% sucrose, and 500 mg/1 of urea or asparagine was suitable for a compact callus with a dry‐to‐fresh‐weight ratio around 8% and on this the tissues grew 15‐ to 17‐fold in 5 weeks.”
t0“Vigna sinensis”
Claim · model-written summary
Enriched Murashige and Skoog medium supported friable Vigna callus with a 3 to 4% dry-to-fresh weight ratio.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- Murashige and Skoog's mediume0
- Outcome
- friable calluse0
- Value
- 3 to 4%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similarly the Murashige and Skoog's medium further enriched with 2% sucrose, 400 mg/1 of urea or a mixture of aspartic acid (750 mg/1), glutamic acid (250 mg/1), asparagine (250 mg/1) and arginine (250 mg/1), was apt for a friable callus with a dry to fresh weight ratio of 3 to 4% and it promoted a 45‐ to 50‐fold increase in growth in 5 weeks.”
t0“Vigna sinensis”
Claim · model-written summary
Enriched Murashige and Skoog medium promoted a 45‐ to 50‐fold Vigna callus growth increase in five weeks.
- Taxon
- Vigna sinensisVigna unguiculata subsp. unguiculatat0
- Treatment
- Murashige and Skoog's mediume0
- Outcome
- growthe0
- Direction
- increasee0
- Value
- 45‐ to 50‐folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Similarly the Murashige and Skoog's medium further enriched with 2% sucrose, 400 mg/1 of urea or a mixture of aspartic acid (750 mg/1), glutamic acid (250 mg/1), asparagine (250 mg/1) and arginine (250 mg/1), was apt for a friable callus with a dry to fresh weight ratio of 3 to 4% and it promoted a 45‐ to 50‐fold increase in growth in 5 weeks.”
t0“Vigna sinensis”
The same questions in other papers
in vitro · growth · Vigna unguiculata (cowpea)Positive and negative results
11 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
12 papers more in this group
See all 16 papers →in vitro · yield · Vigna unguiculata (cowpea)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.