Abstract
Glutathione S-transferases (GSTs) are a family of multifunctional enzymes that play critical roles in the detoxification of xenobiotics and the protection of tissues against oxidative damage. GSTs are important enzymes in plant responses to a number of environmental stresses including herbicides and pathogen attack. Ocs elements are a group of related, 20 bp promoter elements which have been exploited by some plant pathogens to express genes in plants. Ocs elements have also been found to regulate the expression of a plant GST promoter. An Arabidopsis GST gene, called GST6 has been isolated. GST6 expression is under tissue-specific control and is induced following treatment with auxin, salicylic acid and H2O2. The GST6 promoter contains a binding site for two Arabidopsis ocs element binding factors (OBF), that has some sequence homology to ocs element sequences. Interestingly, OBP1 (OBF binding protein), a DNA-binding protein that was isolated by screening an Arabidopsis cDNA library with a labeled OBF protein as a probe, binds next to the OBF-binding site on the GST6 promoter. OBP1 was able to significantly stimulate the binding of OBF proteins to the GST6 promoter, raising the possibility that interactions between the OBP1 and OBF proteins may be important for GST6 expression.
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Claims 8
Claim · model-written summary
The Arabidopsis GST6 gene was isolated.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- GST6e0
- Outcome
- GST6 has been isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An Arabidopsis GST gene, called GST6 has been isolated”
Claim · model-written summary
GST6 expression is tissue specific.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- GST6e0
- Outcome
- GST6 expression is under tissue-specific controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GST6 expression is under tissue-specific control”
t0“Arabidopsis”
Claim · model-written summary
Auxin induced GST6 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GST6 expression is under tissue-specific control and is induced following treatment with auxin, salicylic acid and H2O2”
t0“Arabidopsis”
Claim · model-written summary
Salicylic acid induced GST6 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- salicylic acide0
- Gene
- GST6e0
- Outcome
- GST6 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GST6 expression is under tissue-specific control and is induced following treatment with auxin, salicylic acid and H2O2”
t0“Arabidopsis”
Claim · model-written summary
H2O2 induced GST6 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GST6 expression is under tissue-specific control and is induced following treatment with auxin, salicylic acid and H2O2”
t0“Arabidopsis”
Claim · model-written summary
The GST6 promoter has a site for two Arabidopsis OBF proteins with some ocs-element sequence homology.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- GST6e0
- Outcome
- binding site for two Arabidopsis ocs element binding factors (OBF)e0
- Qualifier
- some sequence homology to ocs element sequencese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The GST6 promoter contains a binding site for two Arabidopsis ocs element binding factors (OBF), that has some sequence homology to ocs element sequences”
Claim · model-written summary
OBP1 binds adjacent to the OBF site on the GST6 promoter.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- OBP1e0
- Outcome
- binds next to the OBF-binding site on the GST6 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OBP1 (OBF binding protein), a DNA-binding protein that was isolated by screening an Arabidopsis cDNA library with a labeled OBF protein as a probe, binds next to the OBF-binding site on the GST6 promoter”
Claim · model-written summary
OBP1 significantly stimulated OBF protein binding to the GST6 promoter.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- OBP1e0
- Gene
- OBP1e0
- Outcome
- binding of OBF proteins to the GST6 promotere0
- Direction
- stimulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OBP1 was able to significantly stimulate the binding of OBF proteins to the GST6 promoter”
t0“Arabidopsis”
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