Abstract
In order to reveal the aluminum (Al) stress tolerance mechanisms in alfalfa plant at low pH soil, a proteomic approach has been conducted. Alfalfa plants were exposed to Al stress for 5 days. The plant growth and total chlorophyll content are greatly affected by Al stress. The malondialdehyde (MDA) and $H_2O_2$ contents were increased in a low amount but free proline and soluble sugar contents, and the DPPH-radical scavenging activity were highly increased. These results indicate that antioxidant activity (DPPH activity) and osmoprotectants (proline and sugar) may involve in ROS ($H_2O_2$) homeostasis under Al stress. In proteomic analysis, over 500 protein spots were detected by 2-dimentional gel electrophoresis analysis. Total 17 Al stress-induced proteins were identified, of which 8 protein spots were up-regulated and 9 were down-regulated. The differential expression patterns of protein spots were selected and analyzed by the peptide mass fingerprinting (PMF) using MALDI-TOF MS analysis. Three protein spots corresponding to Rubisco were significantly down-regulated whereas peroxiredoxin and glutamine synthetase were up-regulated in response to Al stress. The different regulation patterns of identified proteins were involved in energy metabolism and antioxidant / ROS detoxification during Al stress in alfalfa. Taken together, these results provide new insight to understand the molecular mechanisms of alfalfa plant in terms of Al stress tolerance.
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Claims 10
Claim · model-written summary
Al stress greatly affected alfalfa growth and total chlorophyll content.
- Taxon
- Alfalfa
Medicago sativat0 - Treatment
- Al stresse0
- Outcome
- plant growth and total chlorophyll contente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The plant growth and total chlorophyll content are greatly affected by Al stress.”
t0“Alfalfa”
e1“The plant growth and total chlorophyll content are greatly affected by Al stress.”
Claim · model-written summary
Malondialdehyde and hydrogen peroxide contents increased slightly under Al stress.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Al stresse1
- Outcome
- malondialdehyde (MDA) and $H_2O_2$ contentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The malondialdehyde (MDA) and $H_2O_2$ contents were increased in a low amount”
t0“Alfalfa”
e1“The plant growth and total chlorophyll content are greatly affected by Al stress.”
Claim · model-written summary
Free proline and soluble sugar contents rose strongly under Al stress.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Al stresse1
- Outcome
- free proline and soluble sugar contentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“free proline and soluble sugar contents, and the DPPH-radical scavenging activity were highly increased.”
t0“Alfalfa”
e1“The plant growth and total chlorophyll content are greatly affected by Al stress.”
Claim · model-written summary
DPPH radical scavenging activity rose strongly under Al stress.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Al stresse1
- Outcome
- DPPH-radical scavenging activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the DPPH-radical scavenging activity were highly increased.”
t0“Alfalfa”
e1“The plant growth and total chlorophyll content are greatly affected by Al stress.”
Claim · model-written summary
Antioxidant activity and osmoprotectants may contribute to ROS homeostasis under Al stress.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- ROS ($H_2O_2$) homeostasise0
- Qualifier
- antioxidant activity (DPPH activity) and osmoprotectants (proline and sugar)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results indicate that antioxidant activity (DPPH activity) and osmoprotectants (proline and sugar) may involve in ROS ($H_2O_2$) homeostasis under Al stress.”
t0“Alfalfa”
e1“The plant growth and total chlorophyll content are greatly affected by Al stress.”
Claim · model-written summary
Proteomic analysis detected over 500 protein spots.
- Taxon
- AlfalfaMedicago sativat0
- Outcome
- protein spotse0
- Value
- over 500e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“over 500 protein spots were detected by 2-dimentional gel electrophoresis analysis.”
t0“Alfalfa”
e1“The plant growth and total chlorophyll content are greatly affected by Al stress.”
Claim · model-written summary
Seventeen Al stress induced proteins were identified, eight upregulated and nine downregulated.
- Taxon
- AlfalfaMedicago sativat0
- Outcome
- Al stress-induced proteinse0
- Value
- 17e0
- Qualifier
- 8 protein spots were up-regulated and 9 were down-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Total 17 Al stress-induced proteins were identified, of which 8 protein spots were up-regulated and 9 were down-regulated.”
t0“Alfalfa”
e1“The plant growth and total chlorophyll content are greatly affected by Al stress.”
Claim · model-written summary
Three Rubisco protein spots were significantly downregulated under Al stress.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- Al stresse0
- Gene
- Rubiscoe0
- Outcome
- protein spots corresponding to Rubiscoe0
- Direction
- down-regulatede0
- Value
- Three protein spotse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Three protein spots corresponding to Rubisco were significantly down-regulated whereas peroxiredoxin and glutamine synthetase were up-regulated in response to Al stress.”
t0“Alfalfa”
e1“The plant growth and total chlorophyll content are greatly affected by Al stress.”
Claim · model-written summary
Peroxiredoxin was upregulated in response to Al stress.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- Al stresse0
- Gene
- peroxiredoxine0
- Outcome
- peroxiredoxine0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“peroxiredoxin and glutamine synthetase were up-regulated in response to Al stress.”
t0“Alfalfa”
e1“The plant growth and total chlorophyll content are greatly affected by Al stress.”
Claim · model-written summary
Glutamine synthetase was upregulated in response to Al stress.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- Al stresse0
- Gene
- glutamine synthetasee0
- Outcome
- glutamine synthetasee0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“peroxiredoxin and glutamine synthetase were up-regulated in response to Al stress.”
t0“Alfalfa”
e1“The plant growth and total chlorophyll content are greatly affected by Al stress.”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.