Abstract
Flavonols are important ultraviolet light protectants in many plants and contribute substantially to the quality and health-promoting effects of fruits and derived plant products. To study the regulation of flavonol synthesis in fruit, we isolated and characterized the grapevine (Vitis vinifera 'Shiraz') R2R3-MYB transcription factor VvMYBF1. Transient reporter assays established VvMYBF1 to be a specific activator of flavonol synthase1 (VvFLS1) and several other promoters of grapevine and Arabidopsis (Arabidopsis thaliana) genes involved in flavonol synthesis. Expression of VvMYBF1 in the Arabidopsis mutant myb12 resulted in complementation of its flavonol-deficient phenotype and confirmed the function of VvMYBF1 as a transcriptional regulator of flavonol synthesis. Transcript analysis of VvMYBF1 throughout grape berry development revealed its expression during flowering and in skins of ripening berries, which correlates with the accumulation of flavonols and expression of VvFLS1. In addition to its developmental regulation, VvMYBF1 expression was light inducible, implicating VvMYBF1 in the control of VvFLS1 transcription. Sequence analysis of VvMYBF1 and VvFLS1 indicated conserved putative light regulatory units in promoters of both genes from different cultivars. By analysis of the VvMYBF1 amino acid sequence, we identified the previously described SG7 domain and an additional sequence motif conserved in several plant MYB factors. The described motifs have been used to identify MYB transcription factors from other plant species putatively involved in the regulation of flavonol biosynthesis. To our knowledge, this is the first functional characterization of a light-inducible MYB transcription factor controlling flavonol synthesis in fruit.
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Claims 10
Claim · model-written summary
VvMYBF1 specifically activated VvFLS1 and other flavonol synthesis promoters.
VitisOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient reporter assays established VvMYBF1 to be a specific activator of flavonol synthase1 (VvFLS1) and several other promoters of grapevine and Arabidopsis (Arabidopsis thaliana) genes involved in flavonol synthesis”
Claim · model-written summary
VvMYBF1 expression complemented the flavonol-deficient Arabidopsis myb12 mutant.
- Taxon
- Arabidopsis
Arabidopsise0 - Material
- myb12e0
- Treatment
- Expression of VvMYBF1e0
- Gene
- VvMYBF1e0
- Outcome
- complementation of its flavonol-deficient phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of VvMYBF1 in the Arabidopsis mutant myb12 resulted in complementation of its flavonol-deficient phenotype”
Claim · model-written summary
VvMYBF1 expression occurred during flowering and in ripening berry skins.
Verbatim
e0“Transcript analysis of VvMYBF1 throughout grape berry development revealed its expression during flowering and in skins of ripening berries”
Claim · model-written summary
VvMYBF1 expression correlated with flavonol accumulation.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which correlates with the accumulation of flavonols and expression of VvFLS1”
t0“Grapevine”
Claim · model-written summary
VvMYBF1 expression correlated with VvFLS1 expression.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which correlates with the accumulation of flavonols and expression of VvFLS1”
t0“Grapevine”
Claim · model-written summary
Light induced VvMYBF1 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VvMYBF1 expression was light inducible”
t0“Grapevine”
Claim · model-written summary
VvMYBF1 and VvFLS1 promoters contained conserved putative light regulatory units across cultivars.
- Material
- different cultivarse0
- Gene
- VvMYBF1 and VvFLS1e0
- Outcome
- conserved putative light regulatory unitse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sequence analysis of VvMYBF1 and VvFLS1 indicated conserved putative light regulatory units in promoters of both genes from different cultivars”
t0“Grapevine”
Claim · model-written summary
VvMYBF1 protein contained the SG7 domain.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified the previously described SG7 domain and an additional sequence motif conserved in several plant MYB factors”
e1“VvMYBF1 amino acid sequence”
t0“Grapevine”
Claim · model-written summary
VvMYBF1 protein contained another motif conserved in plant MYB factors.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified the previously described SG7 domain and an additional sequence motif conserved in several plant MYB factors”
e1“VvMYBF1 amino acid sequence”
t0“Grapevine”
Claim · model-written summary
These motifs were used to identify putative flavonol regulatory MYB factors in other plants.
Not reportedValue
Verbatim
e0“The described motifs have been used to identify MYB transcription factors from other plant species putatively involved in the regulation of flavonol biosynthesis”
The same questions in other papers
light · molecular: expression · VitisPositive and negative results
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- negative
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.