Abstract
• Barley (Hordeum vulgare L.) Mildew resistance locus a (Mla) confers allele-specific interactions with natural variants of the ascomycete fungus Blumeria graminis f. sp. hordei (Bgh), the causal agent of powdery mildew disease. Significant reprogramming of Mla-mediated gene expression occurs upon infection by this obligate biotrophic pathogen. • We utilized a proteomics-based approach, combined with barley mla, required for Mla12 resistance1 (rar1), and restoration of Mla resistance1 (rom1) mutants, to identify components of Mla-directed signaling. • Loss-of-function mutations in Mla and Rar1 both resulted in the reduced accumulation of chloroplast copper/zinc superoxide dismutase 1 (HvSOD1), whereas loss of function in Rom1 re-established HvSOD1 levels. In addition, both Mla and Rom1 negatively regulated hvu-microRNA398 (hvu-miR398), and up-regulation of miR398 was coupled to reduced HvSOD1 expression. Barley stripe mosaic virus (BSMV)-mediated over-expression of both barley and Arabidopsis miR398 repressed accumulation of HvSOD1, and BSMV-induced gene silencing of HvSod1 impeded Mla-triggered H₂O₂ and hypersensitive reaction (HR) at barley-Bgh interaction sites. • These data indicate that Mla- and Rom1-regulated hvu-miR398 represses HvSOD1 accumulation, influencing effector-induced HR in response to the powdery mildew fungus.
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Claims 11
Claim · model-written summary
Loss-of-function Mla mutations reduced chloroplast HvSOD1 accumulation.
- Treatment
- Loss-of-function mutations in Mlae0
- Gene
- Mlae0
- Outcome
- accumulation of chloroplast copper/zinc superoxide dismutase 1 (HvSOD1)e0
- Direction
- reducede0
Not reportedTaxonComparator
Verbatim
e0“Loss-of-function mutations in Mla and Rar1 both resulted in the reduced accumulation of chloroplast copper/zinc superoxide dismutase 1 (HvSOD1), whereas loss of function in Rom1 re-established HvSOD1 levels.”
Claim · model-written summary
Loss-of-function Rar1 mutations reduced chloroplast HvSOD1 accumulation.
- Treatment
- Loss-of-function mutations in Mla and Rar1e0
- Gene
- Rar1e0
- Outcome
- accumulation of chloroplast copper/zinc superoxide dismutase 1 (HvSOD1)e0
- Direction
- reducede0
Not reportedTaxonComparator
Verbatim
e0“Loss-of-function mutations in Mla and Rar1 both resulted in the reduced accumulation of chloroplast copper/zinc superoxide dismutase 1 (HvSOD1), whereas loss of function in Rom1 re-established HvSOD1 levels.”
Claim · model-written summary
Rom1 loss of function restored HvSOD1 levels.
Not reportedTaxonComparator
Verbatim
e0“whereas loss of function in Rom1 re-established HvSOD1 levels.”
Claim · model-written summary
Mla negatively regulated hvu-miR398.
Not reportedTaxon
Verbatim
e0“In addition, both Mla and Rom1 negatively regulated hvu-microRNA398 (hvu-miR398), and up-regulation of miR398 was coupled to reduced HvSOD1 expression.”
Claim · model-written summary
Rom1 negatively regulated hvu-miR398.
Not reportedTaxon
Verbatim
e0“In addition, both Mla and Rom1 negatively regulated hvu-microRNA398 (hvu-miR398), and up-regulation of miR398 was coupled to reduced HvSOD1 expression.”
Claim · model-written summary
Higher miR398 was associated with reduced HvSOD1 expression.
Not reportedTaxon
Verbatim
e0“up-regulation of miR398 was coupled to reduced HvSOD1 expression.”
Claim · model-written summary
BSMV-mediated barley miR398 overexpression repressed HvSOD1 accumulation.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Treatment
- over-expression of both barley and Arabidopsis miR398e0
- Gene
- miR398e0
- Outcome
- accumulation of HvSOD1e0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Barley stripe mosaic virus (BSMV)-mediated over-expression of both barley and Arabidopsis miR398 repressed accumulation of HvSOD1,”
Claim · model-written summary
BSMV-mediated Arabidopsis miR398 overexpression repressed barley HvSOD1 accumulation.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- over-expression of both barley and Arabidopsis miR398e0
- Gene
- Arabidopsis miR398e0
- Outcome
- accumulation of HvSOD1e0
- Direction
- repressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Barley stripe mosaic virus (BSMV)-mediated over-expression of both barley and Arabidopsis miR398 repressed accumulation of HvSOD1,”
Claim · model-written summary
Silencing HvSod1 impeded Mla-triggered H₂O₂ at barley-Bgh interaction sites.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- BSMV-induced gene silencing of HvSod1e0
- Interactor
- Bghe0pathogen
- Gene
- HvSod1e0
- Outcome
- Mla-triggered H₂O₂e0
- Direction
- impedede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BSMV-induced gene silencing of HvSod1 impeded Mla-triggered H₂O₂ and hypersensitive reaction (HR) at barley-Bgh interaction sites.”
Claim · model-written summary
Silencing HvSod1 impeded the Mla-triggered hypersensitive reaction at barley-Bgh interaction sites.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- BSMV-induced gene silencing of HvSod1e0
- Interactor
- Bghe0pathogen
- Gene
- HvSod1e0
- Outcome
- hypersensitive reaction (HR)e0
- Direction
- impedede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BSMV-induced gene silencing of HvSod1 impeded Mla-triggered H₂O₂ and hypersensitive reaction (HR) at barley-Bgh interaction sites.”
Claim · model-written summary
The authors infer that Mla- and Rom1-regulated hvu-miR398 represses HvSOD1 and influences effector-induced hypersensitive response.
- Interactor
- powdery mildew funguse0pathogen
- Gene
- hvu-miR398e0
- Outcome
- HvSOD1 accumulatione0
- Direction
- repressese0
Not reportedTaxon
Verbatim
e0“These data indicate that Mla- and Rom1-regulated hvu-miR398 represses HvSOD1 accumulation, influencing effector-induced HR in response to the powdery mildew fungus.”
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