Paper · 1983 · PLANT PHYSIOLOGY

Nickel Is Not Required for Apourease Synthesis in Soybean Seeds

Rodney G. Winkler; Joseph C. Polacco; David L. Eskew; et al.

8
claims
17
verbatim quotes
57
citations
1
research areas
✓
Open access

Abstract

Soybeans (Glycine max L. Merr. cv ;Maple Presto') harvested from plants cultured in nickel-free medium had <0.005% the activity of nickel-sufficient beans and only 15% the activity of a urease-null variety, Itachi. However, whereas Itachi has no detectable urease protein, nickel-free beans of the variety Maple Presto exhibit normal or near normal levels of urease apoprotein. Thus, nickel isn't necessary for urease apoprotein synthesis. The apoprotein wasn't activated by nickel in vitro but, upon seed imbibition of nickel, urease was partially activated. This in vivo activation was not inhibited by cychoheximide.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Nickel-free beans had less than 0.005% of the urease activity of nickel-sufficient beans.

Taxon
Soybean SeedsGlycine maxt0
Treatment
nickel-freee2
Outcome
activitye0
Direction
<e0
Value
<0.005%e0
Comparator
nickel-sufficient beanse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Soybean Seeds”

✓ located verbatim in the source · title, characters 50–63 · show in text ↑
e0

“had <0.005% the activity of nickel-sufficient beans and only 15% the activity of a urease-null variety, Itachi”

✓ located verbatim in the source · abstract, characters 103–213 · show in text ↑
e2

“nickel-free beans of the variety Maple Presto exhibit normal or near normal levels of urease apoprotein”

✓ located verbatim in the source · abstract, characters 273–376 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Nickel-free beans had 15% of the urease activity of the Itachi variety.

Taxon
Soybean SeedsGlycine maxt0
Outcome
activitye0
Direction
onlye0
Value
15%e0
Comparator
Itachie0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Soybean Seeds”

✓ located verbatim in the source · title, characters 50–63 · show in text ↑
e0

“had <0.005% the activity of nickel-sufficient beans and only 15% the activity of a urease-null variety, Itachi”

✓ located verbatim in the source · abstract, characters 103–213 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Itachi had no detectable urease protein.

Taxon
Soybean SeedsGlycine maxt0
Material
Itachie1
Gene
urease proteine1
Outcome
no detectable urease proteine1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Soybean Seeds”

✓ located verbatim in the source · title, characters 50–63 · show in text ↑
e1

“Itachi has no detectable urease protein”

✓ located verbatim in the source · abstract, characters 232–271 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Nickel-free Maple Presto beans had normal or near-normal urease apoprotein levels.

Taxon
Soybean SeedsGlycine maxt0
Material
Maple Prestoe2
Gene
urease apoproteine2
Outcome
levels of urease apoproteine2
Direction
normal or near normale2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Soybean Seeds”

✓ located verbatim in the source · title, characters 50–63 · show in text ↑
e2

“nickel-free beans of the variety Maple Presto exhibit normal or near normal levels of urease apoprotein”

✓ located verbatim in the source · abstract, characters 273–376 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

Nickel is not required for urease apoprotein synthesis.

Taxon
Soybean SeedsGlycine maxt0
Gene
urease apoproteine3
Outcome
urease apoprotein synthesise3
Qualifier
nickel isn't necessarye3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Soybean Seeds”

✓ located verbatim in the source · title, characters 50–63 · show in text ↑
e3

“nickel isn't necessary for urease apoprotein synthesis”

✓ located verbatim in the source · abstract, characters 384–438 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Nickel did not activate the apoprotein in vitro.

Taxon
Soybean SeedsGlycine maxt0
Treatment
nickel in vitroe4
Outcome
apoproteine4
Direction
wasn't activatede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Soybean Seeds”

✓ located verbatim in the source · title, characters 50–63 · show in text ↑
e4

“The apoprotein wasn't activated by nickel in vitro but, upon seed imbibition of nickel, urease was partially activated”

✓ located verbatim in the source · abstract, characters 440–558 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Seed imbibition of nickel partially activated urease.

Taxon
Soybean SeedsGlycine maxt0
Treatment
seed imbibition of nickele4
Gene
ureasee4
Outcome
ureasee4
Direction
partially activatede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Soybean Seeds”

✓ located verbatim in the source · title, characters 50–63 · show in text ↑
e4

“The apoprotein wasn't activated by nickel in vitro but, upon seed imbibition of nickel, urease was partially activated”

✓ located verbatim in the source · abstract, characters 440–558 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Cychoheximide did not inhibit urease activation in vivo.

Taxon
Soybean SeedsGlycine maxt0
Treatment
cychoheximidee5
Outcome
in vivo activatione5
Direction
not inhibitede5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“Soybean Seeds”

✓ located verbatim in the source · title, characters 50–63 · show in text ↑
e5

“This in vivo activation was not inhibited by cychoheximide”

✓ located verbatim in the source · abstract, characters 560–618 · show in text ↑

nickel atom · molecular: metabolites · Glycine max (soybean)Positive and negative results

5 papers: 3 positive, 2 negative or no effect

1 paper in the same direction as this paper · 3 in the opposite direction

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

in vitro · molecular: metabolites · Glycine max (soybean)Positive and negative results

13 papers: 7 positive, 6 negative or no effect

5 papers in the same direction as this paper · 7 in the opposite direction

  1. positive
  2. positive
  3. positive

9 papers more in this group

See all 13 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.