Abstract
Incubation of soybean cell suspension cultures in B-5 medium containing 0.4 ᴍ sucrose caused induction of chalcone synthase (CHS) up to a specific activity of 8 µkat/kg. CHS from these cultures was purified to apparent homogeneity by a 5-step procedure. Isoelectric focussing of pure CHS gave a major band at pH 5.45 and two weaker bands at pH 5.35 and 5.5. At least the bands at pH 5.35 and 5.45 had CHS activity. Analysis of pure CHS by two-dimensional electrophoresis gave a set ot six proteins with a M, of 40 kDa and pIs between 6.0 and 6.6. One-dimensional PAGE of CHS under non-denaturing conditions gave three closely spaced protein bands. A specific antibody was raised against soybean CHS which cross-reacted with parsley CHS. Attempts to find synthesis of deoxychalconc or of the corresponding 7.4′-dihydroxyflavanonc with CHS of different purification stages and with various cofactors failed.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Sucrose-containing B-5 medium induced chalcone synthase activity in soybean cultures up to 8 µkat/kg.
- Taxon
- soybean
Glycine maxe0 - Treatment
- B-5 medium containing 0.4 ᴍ sucrosee0
- Gene
- chalcone synthase (CHS)e0
- Outcome
- chalcone synthase (CHS)e0
- Direction
- inductione0
- Value
- 8 µkat/kge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incubation of soybean cell suspension cultures in B-5 medium containing 0.4 ᴍ sucrose caused induction of chalcone synthase (CHS) up to a specific activity of 8 µkat/kg.”
Claim · model-written summary
Chalcone synthase was purified to apparent homogeneity by a five-step procedure.
- Taxon
- SoybeanGlycine maxt0
- Gene
- CHSe0
- Outcome
- apparent homogeneitye0
- Value
- 5-stepe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CHS from these cultures was purified to apparent homogeneity by a 5-step procedure.”
t0“Soybean”
Claim · model-written summary
Pure CHS had a major isoelectric band at pH 5.45 and weaker bands at pH 5.35 and 5.5.
- Taxon
- SoybeanGlycine maxt0
- Gene
- CHSe0
- Outcome
- major bande0
- Value
- pH 5.45e0
- Qualifier
- two weaker bands at pH 5.35 and 5.5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Isoelectric focussing of pure CHS gave a major band at pH 5.45 and two weaker bands at pH 5.35 and 5.5.”
t0“Soybean”
Claim · model-written summary
The bands at pH 5.35 and 5.45 had CHS activity.
- Taxon
- SoybeanGlycine maxt0
- Gene
- CHSe0
- Outcome
- CHS activitye0
- Qualifier
- At least the bands at pH 5.35 and 5.45e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At least the bands at pH 5.35 and 5.45 had CHS activity.”
t0“Soybean”
Claim · model-written summary
Two-dimensional electrophoresis resolved six proteins of 40 kDa with pIs from 6.0 to 6.6.
- Taxon
- SoybeanGlycine maxt0
- Gene
- CHSe0
- Outcome
- a set ot six proteinse0
- Value
- 40 kDae0
- Qualifier
- pIs between 6.0 and 6.6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of pure CHS by two-dimensional electrophoresis gave a set ot six proteins with a M, of 40 kDa and pIs between 6.0 and 6.6.”
t0“Soybean”
Claim · model-written summary
Non-denaturing PAGE yielded three closely spaced CHS protein bands.
- Taxon
- SoybeanGlycine maxt0
- Gene
- CHSe0
- Outcome
- three closely spaced protein bandse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One-dimensional PAGE of CHS under non-denaturing conditions gave three closely spaced protein bands.”
t0“Soybean”
Claim · model-written summary
An antibody to soybean CHS cross-reacted with parsley CHS.
- Taxon
- soybeanGlycine maxe0
- Gene
- CHSe0
- Outcome
- cross-reacted with parsley CHSe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A specific antibody was raised against soybean CHS which cross-reacted with parsley CHS.”
Claim · model-written summary
Synthesis of deoxychalconc or the corresponding flavanone was not detected with CHS preparations and cofactors.
- Taxon
- SoybeanGlycine maxt0
- Gene
- CHSe0
- Outcome
- synthesis of deoxychalconc or of the corresponding 7.4′-dihydroxyflavanonce0
- Qualifier
- with CHS of different purification stages and with various cofactorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Attempts to find synthesis of deoxychalconc or of the corresponding 7.4′-dihydroxyflavanonc with CHS of different purification stages and with various cofactors failed.”
t0“Soybean”
The same questions in other papers
sucrose · molecular: metabolites · Glycine max (soybean)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →in vitro · molecular: metabolites · Glycine max (soybean)Positive and negative results
6 papers in the same direction as this paper · 5 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- negative
- negative
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.