Paper · 1982 · Canadian Journal of Botany

Formation of epicuticular wax and its effect on water loss in cabbage plants regenerated from shoot-tip culture

E. Sutter; R. W. Langhans

5
claims
9
verbatim quotes
121
citations
3
research areas
—
Not open access

Abstract

Observation by scanning electron microscopy (SEM) revealed that cabbage plants in vitro had no structured epicuticular wax whereas plants grown in a growth chamber or in the greenhouse had considerable amounts of structured epicuticular wax. Plants grown in vitro developed increasing amounts of structured epicuticular wax after transfer to the greenhouse. Water loss per unit leaf area was greater in plants recently removed from in vitro conditions than in either plants grown in the growth chamber or in cultured plants that had been hardened off. Epicuticular wax was formed in plants grown in vitro at 35% relative humidity indicating that relative humidity is an important factor in the in vitro development of wax. The contribution of light to the formation of epicuticular wax and the relationship of the wax to survival of the plants when they are removed from in vitro culture are discussed.

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Claims 5

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

In vitro cabbage plants lacked structured epicuticular wax.

Outcome
structured epicuticular waxe0
Direction
noe0
Setting
in vitroe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“cabbage plants in vitro had no structured epicuticular wax”

✓ located verbatim in the source · abstract, characters 64–122 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Growth chamber and greenhouse plants had considerable structured epicuticular wax.

Outcome
structured epicuticular waxe0
Direction
considerablee0
Setting
growth chamber or in the greenhousee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“plants grown in a growth chamber or in the greenhouse had considerable amounts of structured epicuticular wax”

✓ located verbatim in the source · abstract, characters 131–240 · show in text ↑
t0

“cabbage plants”

✓ located verbatim in the source · title, characters 62–76 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In vitro plants developed increasing structured wax after greenhouse transfer.

Treatment
transfer to the greenhousee0
Outcome
structured epicuticular waxe0
Direction
increasinge0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Plants grown in vitro developed increasing amounts of structured epicuticular wax after transfer to the greenhouse”

✓ located verbatim in the source · abstract, characters 242–356 · show in text ↑
t0

“cabbage plants”

✓ located verbatim in the source · title, characters 62–76 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Recently removed in vitro plants lost more water per leaf area than growth chamber plants or hardened plants.

Outcome
Water loss per unit leaf areae0
Direction
greatere0
Comparator
plants grown in the growth chambere0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Water loss per unit leaf area was greater in plants recently removed from in vitro conditions than in either plants grown in the growth chamber or in cultured plants that had been hardened off”

✓ located verbatim in the source · abstract, characters 358–550 · show in text ↑
t0

“cabbage plants”

✓ located verbatim in the source · title, characters 62–76 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

In vitro plants formed epicuticular wax at 35% relative humidity.

Treatment
35% relative humiditye0
Outcome
Epicuticular waxe0
Setting
in vitroe0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica oleracea var. capitata
Brassica oleracea var. capitata · TaxonThamizhpparithi Maari · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Epicuticular wax was formed in plants grown in vitro at 35% relative humidity”

✓ located verbatim in the source · abstract, characters 552–629 · show in text ↑
t0

“cabbage plants”

✓ located verbatim in the source · title, characters 62–76 · show in text ↑

cultivation system · molecular: metabolites · Brassica oleracea (wild cabbage)Positive and negative results

5 papers: 3 positive, 2 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. positive

1 paper more in this group

See all 5 papers →

humidity · molecular: metabolites · Brassica oleracea (wild cabbage)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.