Abstract
Root-knot nematodes are obligate plant parasites that alter plant cell growth and development by inducing the formation of giant cells for feeding. Nematodes inject secretions from their esophageal glands through their stylet and into plant cells to induce giant cell formation. Meloidogyne javanica chorismate mutase 1 (MjCM-1) is one such esophageal gland protein likely to be secreted from the nematode as giant cells form. MjCM-1 has two domains, an N-terminal chorismate mutase (CM) domain and a C-terminal region of unknown function. It is the N-terminal CM domain of the protein that is the predominant form produced in root-knot nematodes. Transgenic expression of MjCM-1 in soybean hairy roots results in a phenotype of reduced and aborted lateral roots. Histological studies demonstrate the absence of vascular tissue in hairy roots expressing MjCM-1. The phenotype of MjCM-1 expressed at low levels can be rescued by the addition of indole-3-acetic acid (IAA), indicating MjCM-1 overexpression reduces IAA biosynthesis. We propose MjCM-1 lowers IAA by causing a competition for chorismate, resulting in an alteration of chorismate-derived metabolites and, ultimately, in plant cell development. Therefore, we hypothesize that MjCM-1 is involved in allowing nematodes to establish a parasitic relationship with the host plant.
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Claims 7
Claim · model-written summary
The N terminal CM domain was the predominant form in root knot nematodes.
- Taxon
- root-knot nematodes
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=60)
Meloidogynee0 - Gene
- N-terminal CM domaine0
- Outcome
- predominant form producede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_penetrates_a_tomato_root_-_USDA-ARS.jpg?width=500)
Verbatim
e0“It is the N-terminal CM domain of the protein that is the predominant form produced in root-knot nematodes.”
Claim · model-written summary
MjCM-1 expression reduced and aborted soybean lateral roots.
- Taxon
- soybean
Glycine maxe0 - Treatment
- Transgenic expression of MjCM-1e0
- Interactor
- Meloidogyne javanicaMeloidogyne javanicae1parasite
- Gene
- MjCM-1e0
- Outcome
- lateral rootse0
- Direction
- reduced and abortede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic expression of MjCM-1 in soybean hairy roots results in a phenotype of reduced and aborted lateral roots.”
e1“Meloidogyne javanica”
Claim · model-written summary
MjCM-1 expressing hairy roots lacked vascular tissue.
- Taxon
- soybeanGlycine maxe1
- Organ / stage
- hairy rootse0
- Gene
- MjCM-1e0
- Outcome
- absence of vascular tissuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Histological studies demonstrate the absence of vascular tissue in hairy roots expressing MjCM-1.”
e1“soybean hairy roots”
Claim · model-written summary
IAA rescued the low expression MjCM-1 root phenotype.
- Taxon
- soybeanGlycine maxe1
- Treatment
- addition of indole-3-acetic acid (IAA)e0
- Gene
- MjCM-1e0
- Outcome
- phenotype of MjCM-1 expressed at low levelse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The phenotype of MjCM-1 expressed at low levels can be rescued by the addition of indole-3-acetic acid (IAA)”
e1“soybean hairy roots”
Claim · model-written summary
Authors inferred MjCM-1 overexpression reduces IAA biosynthesis.
- Taxon
- soybeanGlycine maxe1
- Gene
- MjCM-1e0
- Outcome
- IAA biosynthesise0
- Direction
- reducese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicating MjCM-1 overexpression reduces IAA biosynthesis.”
e1“soybean hairy roots”
Claim · model-written summary
Authors proposed competition for chorismate lowers IAA and alters metabolites and cell development.
- Taxon
- soybeanGlycine maxe1
- Gene
- MjCM-1e0
- Compound
- chorismatee0
- Outcome
- plant cell developmente0
- Qualifier
- competition for chorismatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We propose MjCM-1 lowers IAA by causing a competition for chorismate, resulting in an alteration of chorismate-derived metabolites and, ultimately, in plant cell development.”
e1“soybean hairy roots”
Claim · model-written summary
Authors hypothesized MjCM-1 helps nematodes establish parasitism with plants.
Verbatim
e0“we hypothesize that MjCM-1 is involved in allowing nematodes to establish a parasitic relationship with the host plant.”
The same questions in other papers
Meloidogyne javanica · growth · Glycine max (soybean)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
indole-3-acetic acid · molecular: expression · Glycine max (soybean)Results agree
2 papers in the same direction as this paper
- positive
- positive
transgenic · growth · Glycine max (soybean)Positive and negative results
6 papers in the same direction as this paper · 10 in the opposite direction
- positive
- positive
- positive
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.