Abstract
The mechanisms by which the expression of animal cell death suppressors in economically important plants conferred enhanced stress tolerance are not fully understood. In the present work, the effect of expression of animal antiapoptotic gene Ced-9 in soybean hairy roots was evaluated under root hairs and hairy roots death-inducing stress conditions given by i) Bradyrhizobium japonicum inoculation in presence of 50 mM NaCl, and ii) severe salt stress (150 mM NaCl), for 30 min and 3 h, respectively. We have determined that root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels, whereas the cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics. The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments. Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production. Even when our results demonstrate a positive effect of animal cell death suppressors in plant cell ionic and redox homeostasis under cell death-inducing conditions, its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions.
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Claims 7
Claim · model-written summary
Rhizobium inoculation with 50 mM NaCl induced ordered root hair death and increased ROS, MDA and ATP.
- Taxon
- Glycine max L.
Glycine maxt0 - Organ / stage
- root hairse0
- Treatment
- inoculation in presence of 50 mM NaCle0
- Interactor
- Bradyrhizobium japonicum
Bradyrhizobium japonicumt0symbiont - Outcome
- root hairs deathe0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root hairs death induced by inoculation in presence of 50 mM NaCl showed characteristics of ordered process, with increased ROS generation, MDA and ATP levels”
t0“Glycine max L.–Bradyrhizobium japonicum”
Claim · model-written summary
150 mM NaCl induced necrotic-like cell death.
- Taxon
- Glycine max L.Glycine maxt0
- Treatment
- 150 mM NaCl treatmente0
- Outcome
- non-ordered or necrotic-like characteristicse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell death induced by 150 mM NaCl treatment showed non-ordered or necrotic-like characteristics”
t0“Glycine max L.”
Claim · model-written summary
Ced-9 expression inhibited or delayed root hair death under the tested treatments.
- Taxon
- Glycine max L.Glycine maxt0
- Treatment
- expression of Ced-9e0
- Gene
- Ced-9e0
- Outcome
- root hairs deathe0
- Direction
- inhibited or at least delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of Ced-9 inhibited or at least delayed root hairs death under these treatments”
t0“Glycine max L.”
Claim · model-written summary
Ced-9 expression prevented potassium release from hairy roots.
- Taxon
- Glycine max L.Glycine maxt0
- Organ / stage
- Hairy rootse0
- Treatment
- expressing Ced-9e0
- Gene
- Ced-9e0
- Outcome
- potassium releasee0
- Direction
- preventinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production”
t0“Glycine max L.”
Claim · model-written summary
Ced-9 expression increased ATP levels in hairy roots.
- Taxon
- Glycine max L.Glycine maxt0
- Organ / stage
- Hairy rootse0
- Treatment
- expressing Ced-9e0
- Gene
- Ced-9e0
- Outcome
- ATP levelse0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production”
t0“Glycine max L.”
Claim · model-written summary
Ced-9 expression controlled oxidative damage and avoided increased reactive oxygen species production.
- Taxon
- Glycine max L.Glycine maxt0
- Treatment
- expressing Ced-9e0
- Gene
- Ced-9e0
- Outcome
- oxidative damage avoiding the increase of reactive oxygen species productione0
- Direction
- avoiding the increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hairy roots expressing Ced-9 had better homeostasis maintenance, preventing potassium release; increasing the ATP levels and controlling the oxidative damage avoiding the increase of reactive oxygen species production”
t0“Glycine max L.”
Claim · model-written summary
Ced-9 expression drastically inhibited nodule formation even under control conditions.
- Taxon
- Glycine max L.Glycine maxt0
- Treatment
- expressione0
- Outcome
- nodule formatione0
- Direction
- drastically inhibitede0
- Comparator
- control conditionse0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression, contrary to expectations, drastically inhibited nodule formation even under control conditions”
t0“Glycine max L.”
The same questions in other papers
transgenic · growth · Glycine max (soybean)Positive and negative results
9 papers in the same direction as this paper · 6 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- no effect
13 papers more in this group
See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.