Abstract
ABSTRACT The regulation of ribulose‐1,5‐biphosphate carboxlase/oxygenase (Rubisco) and Rubisco activase was followed for 3 months in an experiment studying the effects of ozone and water stress on Aleppo pine. Rubisco activity was shown to be reduced by 30% in the presence of ozone, whereas no significant effect of water stress was noticed. The effect of combined stresses on Rubisco activity was similar to the effect of ozone. The changes in protein quantity of Rubisco large subunit (LSU) and Rubisco activase (RCA), compared with control plants, were similar to that of the Rubisco activity. Using homologous probes obtained by reverse transcription (RT)‐polymerase chain reaction (PCR), rbcL and rca transcript quantities were quantified during the course of the experiment. RbcL and rca mRNA quantities decreased in ozone and after drought. Changes in rbcL transcript quantity in needles subjected to the combination of ozone and drought were similar to the ones detected when drought was applied alone. On the contrary, the pattern of rca changes under the combination of the two stresses was similar to that of ozone applied alone. A positive correlation existed between the effects of ozone on Rubisco activase and Rubisco LSU protein quantities, which was not so obvious by comparing transcript quantities. This could suggest a potential post‐transcriptional coordinated regulation of the two proteins under stress‐imposed conditions.
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Claims 12
Claim · model-written summary
Ozone reduced Rubisco activity by 30%.
- Taxon
- Pinus halepensis M.
Pinus halepensist0 - Treatment
- ozonee0
- Gene
- Rubiscoe0
- Outcome
- Rubisco activitye0
- Direction
- reducede0
- Value
- 30%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rubisco activity was shown to be reduced by 30% in the presence of ozone”
t0“Pinus halepensis M.”
Claim · model-written summary
Water stress had no significant effect on Rubisco activity.
- Taxon
- Pinus halepensis M.Pinus halepensist0
- Treatment
- water stresse0
- Outcome
- Rubisco activitye1
- Direction
- no significant effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no significant effect of water stress was noticed”
e1“Rubisco activity was shown to be reduced by 30% in the presence of ozone”
t0“Pinus halepensis M.”
Claim · model-written summary
Combined ozone and water stress affected Rubisco activity similarly to ozone alone.
- Taxon
- Pinus halepensis M.Pinus halepensist0
- Treatment
- combined stressese0
- Outcome
- Rubisco activitye0
- Direction
- similare0
- Comparator
- ozonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of combined stresses on Rubisco activity was similar to the effect of ozone”
t0“Pinus halepensis M.”
Claim · model-written summary
Rubisco large subunit protein quantity changed similarly to Rubisco activity relative to control plants.
- Taxon
- Pinus halepensis M.Pinus halepensist0
- Gene
- Rubisco large subunit (LSU)e0
- Outcome
- protein quantitye0
- Direction
- similare0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The changes in protein quantity of Rubisco large subunit (LSU) and Rubisco activase (RCA), compared with control plants, were similar to that of the Rubisco activity”
t0“Pinus halepensis M.”
Claim · model-written summary
Rubisco activase protein quantity changed similarly to Rubisco activity relative to control plants.
- Taxon
- Pinus halepensis M.Pinus halepensist0
- Gene
- Rubisco activase (RCA)e0
- Outcome
- protein quantitye0
- Direction
- similare0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The changes in protein quantity of Rubisco large subunit (LSU) and Rubisco activase (RCA), compared with control plants, were similar to that of the Rubisco activity”
t0“Pinus halepensis M.”
Claim · model-written summary
Ozone decreased rbcL and rca mRNA quantities.
- Taxon
- Pinus halepensis M.Pinus halepensist0
- Treatment
- ozonee0
- Outcome
- RbcL and rca mRNA quantitiese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RbcL and rca mRNA quantities decreased in ozone and after drought”
t0“Pinus halepensis M.”
Claim · model-written summary
Drought decreased rbcL and rca mRNA quantities.
- Taxon
- Pinus halepensis M.Pinus halepensist0
- Treatment
- droughte0
- Outcome
- RbcL and rca mRNA quantitiese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RbcL and rca mRNA quantities decreased in ozone and after drought”
t0“Pinus halepensis M.”
Claim · model-written summary
Combined stress changed rbcL transcript quantity similarly to drought alone.
- Taxon
- Pinus halepensis M.Pinus halepensist0
- Organ / stage
- needlese0
- Treatment
- combination of ozone and droughte0
- Outcome
- rbcL transcript quantitye0
- Direction
- similare0
- Comparator
- droughte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Changes in rbcL transcript quantity in needles subjected to the combination of ozone and drought were similar to the ones detected when drought was applied alone”
t0“Pinus halepensis M.”
Claim · model-written summary
Combined stress changed rca transcripts similarly to ozone alone.
- Taxon
- Pinus halepensis M.Pinus halepensist0
- Treatment
- combination of the two stressese0
- Outcome
- rca changese0
- Direction
- similare0
- Comparator
- ozonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the pattern of rca changes under the combination of the two stresses was similar to that of ozone applied alone”
t0“Pinus halepensis M.”
Claim · model-written summary
Ozone effects on Rubisco activase and Rubisco LSU protein quantities were positively correlated.
- Taxon
- Pinus halepensis M.Pinus halepensist0
- Treatment
- ozonee0
- Outcome
- Rubisco activase and Rubisco LSU protein quantitiese0
- Direction
- positive correlatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A positive correlation existed between the effects of ozone on Rubisco activase and Rubisco LSU protein quantities”
t0“Pinus halepensis M.”
Claim · model-written summary
The corresponding transcript quantity correlation was less clear.
- Taxon
- Pinus halepensis M.Pinus halepensist0
- Treatment
- ozonee1
- Outcome
- transcript quantitiese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which was not so obvious by comparing transcript quantities”
e1“A positive correlation existed between the effects of ozone on Rubisco activase and Rubisco LSU protein quantities”
t0“Pinus halepensis M.”
Claim · model-written summary
The authors suggest coordinated post-transcriptional regulation of the proteins under stress.
- Taxon
- Pinus halepensis M.Pinus halepensist0
- Outcome
- coordinated regulation of the two proteinse0
- Qualifier
- post‐transcriptionale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This could suggest a potential post‐transcriptional coordinated regulation of the two proteins under stress‐imposed conditions”
t0“Pinus halepensis M.”
The same questions in other papers
drought · physiology: photosynthesis · Pinus halepensis (Aleppo pine)Results agree
2 papers in the same direction as this paper
- negative
- negative
ozone · physiology: photosynthesis · Pinus halepensis (Aleppo pine)Results agree
4 papers in the same direction as this paper
- negative
- no effect
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.