Abstract
AIMS: This study aimed to evaluate different packaging strategies to extend the shelf life of a freeze-dried formulation of the biocontrol agent Pantoea agglomerans strain CPA-2. METHODS AND RESULTS: Different materials and atmosphere packaging conditions (vacuum and air) were analysed on formulated P. agglomerans cells stored at 25, 5 and -20°C. Results showed the viability of CPA-2 cells stored at 5 or -20°C was significantly higher than when stored at 25°C. The highest viabilities were observed with the plastic material designated as Bottle 1, in nonvacuum packaging in all storage temperatures: 50% after 3 months at 25°C, 100% after 8 months at 5°C and 100 and 74% after 12 and 18 months, respectively, at -20°C; the final concentration was 10(12) CFU g(-1), a good concentration for a commercial product. The efficacy to control blue and green mould on apples and oranges, respectively, of these packed and stored cells was similar to fresh CPA-2 cells. CONCLUSIONS: This work showed a suitable packaging strategy for a freeze-dried formulation of the CPA-2, providing a good shelf life and efficacy against the major postharvest diseases of apples and citrus based on a plastic bottle stored at cold or frozen storage conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: The last phase of the commercial development process for biocontrol agents is presented in this work. A bacterium-based product that ensures the efficacy, stability and easy application of the antagonist to control postharvest fungal diseases on fruit was successfully obtained.
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Claims 8
Claim · model-written summary
CPA-2 cell viability was significantly higher at 5°C than at 25°C.
Verbatim
e0“viability of CPA-2 cells stored at 5 or -20°C was significantly higher than when stored at 25°C”
t0“blue and green mould on apples and oranges, respectively”
Claim · model-written summary
CPA-2 cell viability was significantly higher at -20°C than at 25°C.
Verbatim
e0“viability of CPA-2 cells stored at 5 or -20°C was significantly higher than when stored at 25°C”
t0“blue and green mould on apples and oranges, respectively”
Claim · model-written summary
Bottle 1 in nonvacuum packaging gave 50% viability after three months at 25°C.
- Taxon
- apples and orangest0
- Treatment
- Bottle 1, in nonvacuum packaginge0
- Outcome
- viabilitiese0
- Direction
- higheste0
- Value
- 50%e0
- Qualifier
- after 3 months at 25°Ce0
Not reportedComparator
Verbatim
e0“The highest viabilities were observed with the plastic material designated as Bottle 1, in nonvacuum packaging in all storage temperatures: 50% after 3 months at 25°C”
t0“blue and green mould on apples and oranges, respectively”
e1“viability of CPA-2 cells”
Claim · model-written summary
Bottle 1 in nonvacuum packaging gave 100% viability after eight months at 5°C.
Verbatim
e0“100% after 8 months at 5°C”
t0“blue and green mould on apples and oranges, respectively”
e1“viability of CPA-2 cells”
Claim · model-written summary
Bottle 1 in nonvacuum packaging gave 100% viability after 12 months at -20°C.
Verbatim
e0“100 and 74% after 12 and 18 months, respectively, at -20°C”
t0“blue and green mould on apples and oranges, respectively”
e1“viability of CPA-2 cells”
Claim · model-written summary
Bottle 1 in nonvacuum packaging gave 74% viability after 18 months at -20°C.
Verbatim
e0“100 and 74% after 12 and 18 months, respectively, at -20°C”
t0“blue and green mould on apples and oranges, respectively”
e1“viability of CPA-2 cells”
Claim · model-written summary
Packed and stored CPA-2 controlled blue mould on apples similarly to fresh cells.
- Taxon
- apples
Malus domesticae0 - Treatment
- packed and stored cellse0
- Interactor
- CPA-2e0biocontrol_agent
- Outcome
- efficacy to control bluee0
- Direction
- similare0
- Comparator
- fresh CPA-2 cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The efficacy to control blue and green mould on apples and oranges, respectively, of these packed and stored cells was similar to fresh CPA-2 cells”
Claim · model-written summary
Packed and stored CPA-2 controlled green mould on oranges similarly to fresh cells.
Verbatim
e0“The efficacy to control blue and green mould on apples and oranges, respectively, of these packed and stored cells was similar to fresh CPA-2 cells”
The same questions in other papers
storage · control · Malus domestica (apple)Positive and negative results
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- negative
- positive
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1 paper more in this group
See all 5 papers →storage · control · Citrus sinensis (sweet orange)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.