Paper · 1997 · Open Access Repository of ICRISAT (International Crops Research Institute for the Semi-Arid Tropics)

An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea

K. R. Shivanna; N. P. Saxena; N. Seetharama

6
claims
17
verbatim quotes
8
citations
1
research areas
✓
Open access

Abstract

Using pollen from a cold tolerant accession ICC8923, best results for pollen germination and pollen tube growth were obtained in medium containing 2.5% sucrose + 10% PEG + 100 mg boric acid + 300 mg calcium nitrate + 200 mg magnesium sulfate + 100 mg potassium nitrate/litre. The results of pollen germination tests using this medium (PEG medium) and Brewbaker & Kwack (BK) medium are reported. In the BK medium, pollen germination was lower and pollen tube growth was largely confined to the first 60 min with tube abnormalities such as swelling and bursting occurring in 3-h-old cultures. In PEG medium pollen germination was consistently high (<80% after 90 min) and pollen tube length was nearly five times greater than that obtained in the BK medium after 90 min.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher.

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The PEG medium gave the best pollen germination and pollen tube growth of chickpea pollen from accession ICC8923.

Taxon
ChickpeaCicer arietinumt0
Material
ICC8923e0
Organ / stage
pollene0
Treatment
PEG mediume1
Outcome
pollen germination and pollen tube growthe0
Direction
best resultse0
Comparator
Brewbaker & Kwack (BK) mediume1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea”

✓ located verbatim in the source · title, characters 0–87 · show in text ↑
e0

“Using pollen from a cold tolerant accession ICC8923, best results for pollen germination and pollen tube growth were obtained in medium containing 2.5% sucrose + 10% PEG + 100 mg boric acid + 300 mg calcium nitrate + 200 mg magnesium sulfate + 100 mg potassium nitrate/litre”

✓ located verbatim in the source · abstract, characters 0–274 · show in text ↑
e1

“The results of pollen germination tests using this medium (PEG medium) and Brewbaker & Kwack (BK) medium are reported”

✓ located verbatim in the source · abstract, characters 276–393 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Pollen germination was lower in the BK medium than in the PEG medium.

Taxon
ChickpeaCicer arietinumt0
Organ / stage
pollene1
Treatment
BK mediume1
Outcome
pollen germinatione1
Direction
lowere1
Comparator
PEG mediume0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea”

✓ located verbatim in the source · title, characters 0–87 · show in text ↑
e0

“The results of pollen germination tests using this medium (PEG medium) and Brewbaker & Kwack (BK) medium are reported”

✓ located verbatim in the source · abstract, characters 276–393 · show in text ↑
e1

“In the BK medium, pollen germination was lower”

✓ located verbatim in the source · abstract, characters 395–441 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

In the BK medium, pollen tube growth was largely confined to the first 60 min.

Taxon
ChickpeaCicer arietinumt0
Organ / stage
pollene0
Treatment
BK mediume0
Outcome
pollen tube growthe1
Qualifier
largely confined to the first 60 mine1

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea”

✓ located verbatim in the source · title, characters 0–87 · show in text ↑
e0

“In the BK medium, pollen germination was lower”

✓ located verbatim in the source · abstract, characters 395–441 · show in text ↑
e1

“pollen tube growth was largely confined to the first 60 min”

✓ located verbatim in the source · abstract, characters 446–505 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

In the BK medium, tube abnormalities such as swelling and bursting occurred in 3-h-old cultures.

Taxon
ChickpeaCicer arietinumt0
Organ / stage
pollene0
Treatment
BK mediume0
Outcome
tube abnormalitiese1
Qualifier
swelling and burstinge1

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea”

✓ located verbatim in the source · title, characters 0–87 · show in text ↑
e0

“In the BK medium, pollen germination was lower”

✓ located verbatim in the source · abstract, characters 395–441 · show in text ↑
e1

“tube abnormalities such as swelling and bursting occurring in 3-h-old cultures”

✓ located verbatim in the source · abstract, characters 511–589 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

In the PEG medium, pollen germination was consistently high, below 80% after 90 min.

Taxon
ChickpeaCicer arietinumt0
Treatment
PEG mediume0
Outcome
pollen germinatione0
Value
<80%e0
Qualifier
after 90 mine0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea”

✓ located verbatim in the source · title, characters 0–87 · show in text ↑
e0

“In PEG medium pollen germination was consistently high (<80% after 90 min)”

✓ located verbatim in the source · abstract, characters 591–665 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pollen tube length in the PEG medium was nearly five times greater than in the BK medium after 90 min.

Taxon
ChickpeaCicer arietinumt0
Organ / stage
Pollent0
Treatment
PEG mediume0
Outcome
pollen tube lengthe1
Direction
greatere1
Value
nearly five timese1
Qualifier
after 90 mine1
Comparator
BK mediume1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cicer arietinum
Cicer arietinum · TaxonSanjay Acharya · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

t0

“An Improvised Medium for In vitro Pollen Germination and Pollen Tube Growth of Chickpea”

✓ located verbatim in the source · title, characters 0–87 · show in text ↑
e0

“In PEG medium pollen germination was consistently high (<80% after 90 min)”

✓ located verbatim in the source · abstract, characters 591–665 · show in text ↑
e1

“pollen tube length was nearly five times greater than that obtained in the BK medium after 90 min”

✓ located verbatim in the source · abstract, characters 670–767 · show in text ↑

drought · yield · Cicer arietinum (chickpea)Results differ

54 papers: 11 positive, 43 negative or no effect

10 papers in the same direction as this paper · 43 in the opposite direction

  1. negative
  2. negative
  3. negative

50 papers more in this group

See all 54 papers →

in vitro · yield · Cicer arietinum (chickpea)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.