Abstract
Temperature is a primary factor affecting the rate of plant development; as the climate warms, extreme temperature events are likely to increasingly affect agriculture. Understanding how to improve crop tolerance to heat stress is a key concern. Wild plants have evolved numerous strategies to tolerate environmental conditions, notably the regulation of root architecture by phytohormones, but the molecular mechanisms of stress resistance are unclear. In this study, we showed that high temperatures could significantly reduce tobacco biomass and change its root architecture, probably through changes in auxin content and distribution. Overexpression of the OsPT8 phosphate transporter enhanced tobacco tolerance to high-temperature stress by changing the root architecture and increased the antioxidant ability. Molecular assays suggested that overexpression of OsPT8 in tobacco significantly increased the expression of auxin synthesis genes NtYUCCA 6, 8 and auxin efflux carriers genes NtPIN 1,2 under high-temperature stress. We also found that the expression levels of auxin response factors NtARF1 and NtARF2 were increased in OsPT8 transgenic tobacco under high-temperature stress, suggesting that OsPT8 regulates auxin signaling in response to high-temperature conditions. Our findings provided new insights into the molecular mechanisms of plant stress signaling and showed that OsPT8 plays a key role in regulating plant tolerance to stress conditions.
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Claims 10
Claim · model-written summary
High temperature significantly reduced tobacco biomass.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- high temperaturese0
- Outcome
- tobacco biomasse0
- Direction
- reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high temperatures could significantly reduce tobacco biomass”
Claim · model-written summary
High temperature changed tobacco root architecture.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- high temperaturese0
- Outcome
- root architecturee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high temperatures could significantly reduce tobacco biomass and change its root architecture”
Claim · model-written summary
OsPT8 overexpression enhanced tobacco heat tolerance.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Overexpression of the OsPT8 phosphate transportere0
- Gene
- OsPT8e0
- Outcome
- tobacco tolerance to high-temperature stresse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of the OsPT8 phosphate transporter enhanced tobacco tolerance to high-temperature stress”
Claim · model-written summary
OsPT8 overexpression changed tobacco root architecture under heat.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Overexpression of the OsPT8 phosphate transportere0
- Gene
- OsPT8e0
- Outcome
- root architecturee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of the OsPT8 phosphate transporter enhanced tobacco tolerance to high-temperature stress by changing the root architecture”
Claim · model-written summary
OsPT8 overexpression increased tobacco antioxidant ability.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Overexpression of the OsPT8 phosphate transportere0
- Gene
- OsPT8e0
- Outcome
- antioxidant abilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of the OsPT8 phosphate transporter enhanced tobacco tolerance to high-temperature stress by changing the root architecture and increased the antioxidant ability.”
Claim · model-written summary
OsPT8 overexpression significantly increased NtYUCCA 6, 8 expression under heat.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- overexpression of OsPT8e0
- Gene
- NtYUCCA 6, 8e0
- Outcome
- expression of auxin synthesis genes NtYUCCA 6, 8e0
- Direction
- increasede0
- Qualifier
- high-temperature stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsPT8 in tobacco significantly increased the expression of auxin synthesis genes NtYUCCA 6, 8 and auxin efflux carriers genes NtPIN 1,2 under high-temperature stress.”
Claim · model-written summary
OsPT8 overexpression significantly increased NtPIN 1,2 expression under heat.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- overexpression of OsPT8e0
- Gene
- NtPIN 1,2e0
- Outcome
- auxin efflux carriers genes NtPIN 1,2e0
- Direction
- increasede0
- Qualifier
- high-temperature stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsPT8 in tobacco significantly increased the expression of auxin synthesis genes NtYUCCA 6, 8 and auxin efflux carriers genes NtPIN 1,2 under high-temperature stress.”
Claim · model-written summary
NtARF1 and NtARF2 expression increased in OsPT8 transgenic tobacco under heat.
- Taxon
- tobaccoNicotiana tabacume0
- Material
- OsPT8 transgenic tobaccoe0
- Gene
- NtARF1 and NtARF2e0
- Outcome
- expression levels of auxin response factors NtARF1 and NtARF2e0
- Direction
- increasede0
- Qualifier
- high-temperature stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression levels of auxin response factors NtARF1 and NtARF2 were increased in OsPT8 transgenic tobacco under high-temperature stress”
Claim · model-written summary
The authors suggest OsPT8 regulates auxin signaling under heat.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- OsPT8e0
- Outcome
- auxin signalinge0
- Qualifier
- high-temperature conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that OsPT8 regulates auxin signaling in response to high-temperature conditions.”
e1“OsPT8 transgenic tobacco under high-temperature stress”
Claim · model-written summary
The authors concluded OsPT8 has a key role in plant stress tolerance.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Gene
- OsPT8e0
- Outcome
- plant tolerance to stress conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsPT8 plays a key role in regulating plant tolerance to stress conditions.”
t0“Nicotiana tabacum”
The same questions in other papers
heat · growth · Nicotiana tabacum (tobacco)Positive and negative results
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- positive
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