Abstract
Effects of four culture media on callus induction, regeneration and number of plants per unit culture were studied with mature seeds from five indica rice genotypes as explants. Based on the morphology, the calli were classified into four types as I to IV. Type I and type II are most suited to initiate suspension cultures or as target material for transformation. Number of plants regenerated per unit culture, formation of easily dissociating cell clusters and frequency of type I and type II calli were highest on NBKNB medium. Thus NBKNB medium is suitable for in vitro culture of even the hitherto recalcitrant indica genotypes.
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Claims 3
Claim · model-written summary
NBKNB medium gave the highest number of plants regenerated per unit culture, cell cluster formation and frequency of type I and type II calli among four media.
- Taxon
- rice
Oryza sativae0 - Material
- indica rice genotypese0
- Organ / stage
- mature seedse0
- Treatment
- NBKNB mediume1
- Outcome
- Number of plants regenerated per unit culture, formation of easily dissociating cell clusters and frequency of type I and type II callie1
- Direction
- higheste1
- Comparator
- four culture mediae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Effects of four culture media on callus induction, regeneration and number of plants per unit culture were studied with mature seeds from five indica rice genotypes as explants”
e1“Number of plants regenerated per unit culture, formation of easily dissociating cell clusters and frequency of type I and type II calli were highest on NBKNB medium”
Claim · model-written summary
NBKNB medium is suitable for in vitro culture of even the recalcitrant indica rice genotypes.
- Taxon
- riceOryza sativae0
- Treatment
- NBKNB mediume2
- Outcome
- is suitable for in vitro culturee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Effects of four culture media on callus induction, regeneration and number of plants per unit culture were studied with mature seeds from five indica rice genotypes as explants”
e2“Thus NBKNB medium is suitable for in vitro culture of even the hitherto recalcitrant indica genotypes”
Claim · model-written summary
Type I and type II calli are most suited to initiate suspension cultures or serve as target material for transformation.
- Taxon
- riceOryza sativae0
- Outcome
- most suited to initiate suspension culturese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Effects of four culture media on callus induction, regeneration and number of plants per unit culture were studied with mature seeds from five indica rice genotypes as explants”
e3“Type I and type II are most suited to initiate suspension cultures or as target material for transformation”
The same questions in other papers
in vitro · molecular: metabolites · Oryza sativa (rice)Positive and negative results
5 papers in the same direction as this paper · 5 in the opposite direction · 2 reporting no difference from an alternative
- negative
- negative
- negative
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.