Abstract
5-Aminolevulinic acid (ALA) accumulation in dark-grown tobacco callus cells in the presence of levulinic acid (LA) was followed under blue or red light or in continuous darkness. Significant formation of ALA continued in the dark. The protochlorophyll- (ide) (Pchl) content of dark-incubated cells remained low because of its turnover. We inferred that the feedback inhibition of ALA synthesis by Pchl would not occur in darkincubated callus cells. ALA formation was enhanced by blue light, and this effect reached saturation at an intensity of about 800 mW.m−2. Neither weak nor strong red light affected ALA formation. Full enhancement of ALA formation by blue light was attained after fairly long continuous illumination of the callus cells. This blue lightenhanced activity of ALA synthesis declined very slowly during the subsequent dark incubation. The blue light enhancement of ALA formation was observed in callus cells supplied with sucrose over a wide range of concentrations. Pchl regeneration in carbon-starved callus cells, supplied with glutamate at various concentrations, was also markedly enhanced by blue light. Respiration of the callus cells was not enhanced by blue light. A possible role of blue light in regulating ALA formation in callus cells is discussed.
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Claims 11
Claim · model-written summary
Significant ALA formation continued in dark-incubated tobacco callus cells.
- Taxon
- tobacco
Nicotiana tabacume0 - Outcome
- formation of ALAe1
- Qualifier
- in the darke1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco callus cells”
e1“Significant formation of ALA continued in the dark”
Claim · model-written summary
The protochlorophyll(ide) content of dark-incubated tobacco cells remained low because of its turnover.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- protochlorophyll- (ide) (Pchl) contente1
- Qualifier
- remained low because of its turnovere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco callus cells”
e1“The protochlorophyll- (ide) (Pchl) content of dark-incubated cells remained low because of its turnover”
Claim · model-written summary
The authors inferred that feedback inhibition of ALA synthesis by Pchl would not occur in dark-incubated callus cells.
- Taxon
- tobaccoNicotiana tabacume0
- Compound
- Pchle1
- Outcome
- feedback inhibition of ALA synthesise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco callus cells”
e1“We inferred that the feedback inhibition of ALA synthesis by Pchl would not occur in darkincubated callus cells”
Claim · model-written summary
Blue light enhanced ALA formation in tobacco callus cells.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- blue lighte1
- Outcome
- ALA formatione1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco callus cells”
e1“ALA formation was enhanced by blue light”
Claim · model-written summary
The enhancing effect of blue light on ALA formation reached saturation at an intensity of about 800 mW.m-2.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- this effect reached saturatione1
- Value
- about 800 mW.m−2e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco callus cells”
e1“this effect reached saturation at an intensity of about 800 mW.m−2”
Claim · model-written summary
Neither weak nor strong red light affected ALA formation in tobacco callus cells.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- red lighte1
- Outcome
- ALA formatione1
- Direction
- no_changeinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco callus cells”
e1“Neither weak nor strong red light affected ALA formation”
Claim · model-written summary
Full enhancement of ALA formation by blue light was attained only after fairly long continuous illumination of the callus cells.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- Full enhancement of ALA formation by blue lighte1
- Qualifier
- after fairly long continuous illuminatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco callus cells”
e1“Full enhancement of ALA formation by blue light was attained after fairly long continuous illumination of the callus cells”
Claim · model-written summary
The blue light-enhanced ALA synthesis activity declined very slowly during subsequent dark incubation.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- blue lightenhanced activity of ALA synthesise1
- Direction
- declined very slowlye1
- Qualifier
- subsequent dark incubatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco callus cells”
e1“This blue lightenhanced activity of ALA synthesis declined very slowly during the subsequent dark incubation”
Claim · model-written summary
The blue light enhancement of ALA formation was observed in callus cells supplied with sucrose over a wide range of concentrations.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- blue lighte1
- Outcome
- ALA formatione1
- Direction
- enhancemente1
- Qualifier
- supplied with sucrose over a wide range of concentrationse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco callus cells”
e1“The blue light enhancement of ALA formation was observed in callus cells supplied with sucrose over a wide range of concentrations”
Claim · model-written summary
Pchl regeneration in carbon-starved callus cells supplied with glutamate was markedly enhanced by blue light.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- blue lighte1
- Outcome
- Pchl regeneratione1
- Direction
- markedly enhancede1
- Qualifier
- carbon-starved callus cells, supplied with glutamate at various concentrationse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco callus cells”
e1“Pchl regeneration in carbon-starved callus cells, supplied with glutamate at various concentrations, was also markedly enhanced by blue light”
Claim · model-written summary
Respiration of the callus cells was not enhanced by blue light.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- blue lighte1
- Outcome
- Respiratione1
- Direction
- not enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco callus cells”
e1“Respiration of the callus cells was not enhanced by blue light”
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See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.