Abstract
Ferredoxin-1 (Fed-1) mRNA contains an internal light response element (iLRE) that destabilizes mRNA when light-grown plants are placed in darkness. mRNAs containing this element dissociate from polyribosomes in the leaves of transgenic tobacco (Nicotiana tabacum) plants transferred to the dark for 2 d. Here, we report in vivo labeling experiments with a chloramphenicol acetyl transferase mRNA fused to the Fed-1 iLRE. Our data indicate that the Fed-1 iLRE mediates a rapid decline in translational efficiency and that iLRE-containing mRNAs dissociate from polyribosomes within 20 min after plants are transferred to darkness. Both events occur before the decline in mRNA abundance, and polyribosome association is rapidly reversible if plants are re-illuminated. These observations support a model in which Fed-1 mRNA in illuminated leaves is stabilized by its association with polyribosomes, and/or by translation. In darkness a large portion of the mRNA dissociates from polyribosomes and is subsequently degraded. We also show that a significant portion of total tobacco leaf mRNA is shifted from polyribosomal to non-polyribosomal fractions after 20 min in the dark, indicating that translation of other mRNAs is also rapidly down-regulated in response to darkness. This class includes some, but not all, cytoplasmic mRNAs encoding proteins involved in photosynthesis.
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Claims 8
Claim · model-written summary
The Fed-1 internal light response element rapidly reduced translational efficiency after darkness.
- Taxon
- tobacco
Nicotiana tabacume2 - Treatment
- transferred to darknesse1
- Gene
- Fed-1 iLREe0
- Outcome
- translational efficiencye0
- Direction
- declinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fed-1 iLRE mediates a rapid decline in translational efficiency”
e1“after plants are transferred to darkness”
e2“tobacco (Nicotiana tabacum) plants”
Claim · model-written summary
iLRE-containing mRNAs dissociated from polyribosomes within 20 minutes of dark transfer.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- transferred to darknesse0
- Gene
- iLREe0
- Outcome
- mRNAs dissociate from polyribosomese0
- Value
- 20 mine0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“iLRE-containing mRNAs dissociate from polyribosomes within 20 min after plants are transferred to darkness”
e1“tobacco (Nicotiana tabacum) plants”
Claim · model-written summary
The translation and polyribosome changes preceded decline in mRNA abundance.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- decline in mRNA abundancee0
- Direction
- declinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both events occur before the decline in mRNA abundance”
e1“tobacco (Nicotiana tabacum) plants”
Claim · model-written summary
Reillumination rapidly reversed mRNA polyribosome association.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- re-illuminatede0
- Outcome
- polyribosome associatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“polyribosome association is rapidly reversible if plants are re-illuminated”
e1“tobacco (Nicotiana tabacum) plants”
Claim · model-written summary
The authors propose that polyribosome association or translation stabilizes Fed-1 mRNA in illuminated leaves.
- Taxon
- tobaccoNicotiana tabacume1
- Gene
- Fed-1 mRNAe0
- Outcome
- stabilizede0
- Qualifier
- association with polyribosomes, and/or by translatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fed-1 mRNA in illuminated leaves is stabilized by its association with polyribosomes, and/or by translation”
e1“tobacco (Nicotiana tabacum) plants”
Claim · model-written summary
In darkness, much Fed-1 mRNA dissociates from polyribosomes and is subsequently degraded in the authors model.
- Taxon
- tobaccoNicotiana tabacume2
- Gene
- Fed-1 mRNAe1
- Outcome
- dissociates from polyribosomes and is subsequently degradede0
- Qualifier
- In darknesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In darkness a large portion of the mRNA dissociates from polyribosomes and is subsequently degraded”
e1“Fed-1 mRNA”
e2“tobacco (Nicotiana tabacum) plants”
Claim · model-written summary
A significant portion of total tobacco leaf mRNA shifted away from polyribosomes after 20 minutes in darkness.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- leafe0
- Treatment
- in the darke0
- Outcome
- total tobacco leaf mRNA is shifted from polyribosomal to non-polyribosomal fractionse0
- Direction
- shiftede0
- Value
- 20 mine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a significant portion of total tobacco leaf mRNA is shifted from polyribosomal to non-polyribosomal fractions after 20 min in the dark”
Claim · model-written summary
Some cytoplasmic mRNAs encoding photosynthesis proteins belonged to the rapidly down-regulated translation class, but others did not.
- Taxon
- tobaccoNicotiana tabacume2
- Outcome
- cytoplasmic mRNAs encoding proteins involved in photosynthesise0
- Direction
- down-regulatede1
- Qualifier
- some, but not alle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This class includes some, but not all, cytoplasmic mRNAs encoding proteins involved in photosynthesis”
e1“translation of other mRNAs is also rapidly down-regulated in response to darkness”
e2“tobacco (Nicotiana tabacum) plants”
The same questions in other papers
light · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
12 papers in the same direction as this paper · 8 in the opposite direction
- positive
- positive
- positive
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.