Abstract
Cucumber (Cucumis sativus L.) seedlings are more sensitive to chilling stress when transferred to low temperature from the night cycle than from the day cycle. However, greater damage occurs when chilling is carried out in light than in dark. Freshly isolated protoplasts are extremely sensitive to damage when chilled at 4 degrees C in light, but suffer significantly less injury when chilled in dark. If freshly isolated protoplasts are pre-chill conditioned at 27 degrees C in either light or dark for a few hours prior to exposure to various chilling stresses, subsequent chilling damage is markedly reduced. Damage to chilled protoplasts also is reduced if cultures are placed in dark instead of light immediately following removal from low temperature. Experiments utilizing the cell wall synthesis inhibitor, dichlorobenzonitrile, showed that cell wall regeneration during the pre-chill conditioning period at 27 degrees C does not appear to be associated with the enhanced chilling tolerance observed in these cultures. The results obtained in this investigation suggest that the physiological properties of cucumber cotyledon protoplasts accurately reflect those of intact seedlings, and hence provide a good system for studies into the mechanism of chilling damage in plants.
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Claims 7
Claim · model-written summary
Cucumber seedlings are more chilling sensitive after transfer from the night cycle than the day cycle.
- Taxon
- Cucumber
Cucumis sativuse0 - Organ / stage
- seedlingse0
- Outcome
- sensitive to chilling stresse0
- Direction
- moree0
- Comparator
- day cyclee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cucumber (Cucumis sativus L.) seedlings are more sensitive to chilling stress when transferred to low temperature from the night cycle than from the day cycle”
Claim · model-written summary
Chilling in light causes greater cucumber damage than chilling in dark.
- Taxon
- CucumberCucumis sativuse1
- Treatment
- chilling is carried out in lighte0
- Outcome
- damagee0
- Direction
- greatere0
- Comparator
- darke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“greater damage occurs when chilling is carried out in light than in dark”
e1“Cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Freshly isolated protoplasts suffer less chilling injury in darkness than light at 4 degrees C.
- Taxon
- CucumberCucumis sativuse1
- Organ / stage
- protoplastse0
- Treatment
- chilled in darke0
- Outcome
- injurye0
- Direction
- lesse0
- Comparator
- chilled at 4 degrees C in lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Freshly isolated protoplasts are extremely sensitive to damage when chilled at 4 degrees C in light, but suffer significantly less injury when chilled in dark”
e1“Cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Pre-chill conditioning at 27 degrees C reduces subsequent chilling damage.
- Taxon
- CucumberCucumis sativuse1
- Organ / stage
- protoplastse0
- Treatment
- pre-chill conditioned at 27 degrees Ce0
- Outcome
- chilling damagee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“If freshly isolated protoplasts are pre-chill conditioned at 27 degrees C in either light or dark for a few hours prior to exposure to various chilling stresses, subsequent chilling damage is markedly reduced”
e1“Cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Placing chilled protoplast cultures in darkness immediately after chilling reduces damage.
- Taxon
- CucumberCucumis sativuse1
- Treatment
- placed in darke0
- Outcome
- Damage to chilled protoplastse0
- Direction
- reducede0
- Comparator
- lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Damage to chilled protoplasts also is reduced if cultures are placed in dark instead of light immediately following removal from low temperature”
e1“Cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Cell wall regeneration during conditioning does not appear associated with enhanced chilling tolerance.
- Taxon
- CucumberCucumis sativuse1
- Organ / stage
- cell walle0
- Outcome
- enhanced chilling tolerancee0
- Qualifier
- does not appear to be associatede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cell wall regeneration during the pre-chill conditioning period at 27 degrees C does not appear to be associated with the enhanced chilling tolerance observed in these cultures”
e1“Cucumber (Cucumis sativus L.) seedlings”
Claim · model-written summary
Cucumber cotyledon protoplast physiology reflects intact seedlings and provides a system for chilling damage studies.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- cotyledon protoplastse0
- Outcome
- good system for studies into the mechanism of chilling damagee0
- Comparator
- intact seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the physiological properties of cucumber cotyledon protoplasts accurately reflect those of intact seedlings, and hence provide a good system for studies into the mechanism of chilling damage in plants”
The same questions in other papers
cold · pest · Cucumis sativus (cucumber)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
light · pest · Cucumis sativus (cucumber)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.