Abstract
In vitro selection of Foeniculum vulgare for salt tolerance was undertaken by the use of somaclonal variation. In this idea, explants of root, hypocotyl and cotyledon of sterilized seedling were transferred to callus and regeneration media with concentrations of 0, 50, 100 and 150 mM of NaCl. After 4 weeks, calli induction, regeneration frequency and calli fresh and dry weights, in both control and stress conditions, were measured. The results showed that salinity caused a significant decrease in the callus induction and shoot regeneration of fennel. However, in the presence of 100 and 150 mM NaCl, the highest frequency of callus induction in hypocotyl and cotyledon explants was recorded on the media supplemented with 1 mg l-1 IAA (Indol-3-Acetic Acid) plus 1 mg l-1 2,4-D (2, 4- dichlorophenoxyacetic acid) and 2 mg l-1 kinetin. Among different growth regulator treatments, the combination of 2 mg l-1 NAA (Naphtaleneacetic acid) and 0.5 mg l-1 kinetin was found to be the most effective for shoot regeneration under stress condition. The highest dose of NaCl (150 mM) inhibited callus induction and shoot regeneration compared to control with 41% and 96% respectively. The calli fresh and dry weights of all explants were decreased with the increas of NaCl concentration. The highest and the lowest of dry and fresh weight of calli were observed in 0 and 150 mM respectively.
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Claims 7
Claim · model-written summary
Salinity decreased callus induction and shoot regeneration of fennel.
- Taxon
- Foeniculum vulgare
Anethum foeniculumt0 - Treatment
- salinitye0
- Outcome
- callus induction and shoot regeneratione0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_Parc_Natural_de_s'Albufera%2C_Spain_2022-04-18_(02).jpg?width=500)
Verbatim
t0“In Vitro Selection of Foeniculum vulgare for Salt Tolerance”
e0“salinity caused a significant decrease in the callus induction and shoot regeneration of fennel”
Claim · model-written summary
The highest NaCl dose (150 mM) inhibited callus induction by 41% compared with control.
- Taxon
- Foeniculum vulgare
Anethum foeniculumt0 - Treatment
- NaCl (150 mM)e1
- Outcome
- callus inductione1
- Direction
- inhibitede1
- Value
- 41%e1
- Comparator
- controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_Parc_Natural_de_s'Albufera%2C_Spain_2022-04-18_(02).jpg?width=500)
Verbatim
t0“In Vitro Selection of Foeniculum vulgare for Salt Tolerance”
e1“The highest dose of NaCl (150 mM) inhibited callus induction and shoot regeneration compared to control with 41% and 96% respectively”
Claim · model-written summary
The highest NaCl dose (150 mM) inhibited shoot regeneration by 96% compared with control.
- Taxon
- Foeniculum vulgare
Anethum foeniculumt0 - Treatment
- NaCl (150 mM)e1
- Outcome
- shoot regeneratione1
- Direction
- inhibitede1
- Value
- 96%e1
- Comparator
- controle1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_Parc_Natural_de_s'Albufera%2C_Spain_2022-04-18_(02).jpg?width=500)
Verbatim
t0“In Vitro Selection of Foeniculum vulgare for Salt Tolerance”
e1“The highest dose of NaCl (150 mM) inhibited callus induction and shoot regeneration compared to control with 41% and 96% respectively”
Claim · model-written summary
Calli fresh and dry weights decreased with increasing NaCl concentration.
- Taxon
- Foeniculum vulgare
Anethum foeniculumt0 - Treatment
- NaCl concentratione2
- Outcome
- calli fresh and dry weightse2
- Direction
- decreasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_Parc_Natural_de_s'Albufera%2C_Spain_2022-04-18_(02).jpg?width=500)
Verbatim
t0“In Vitro Selection of Foeniculum vulgare for Salt Tolerance”
e2“The calli fresh and dry weights of all explants were decreased with the increas of NaCl concentration”
Claim · model-written summary
Dry and fresh weight of calli was highest at 0 mM NaCl and lowest at 150 mM.
- Taxon
- Foeniculum vulgare
Anethum foeniculumt0 - Treatment
- 150 mMe3
- Outcome
- dry and fresh weight of callie3
- Direction
- higheste3
- Qualifier
- the loweste3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_Parc_Natural_de_s'Albufera%2C_Spain_2022-04-18_(02).jpg?width=500)
Verbatim
t0“In Vitro Selection of Foeniculum vulgare for Salt Tolerance”
e3“The highest and the lowest of dry and fresh weight of calli were observed in 0 and 150 mM respectively”
Claim · model-written summary
The IAA, 2,4-D and kinetin medium gave the highest frequency of callus induction in hypocotyl and cotyledon explants under 100 and 150 mM NaCl.
- Taxon
- Foeniculum vulgare
Anethum foeniculumt0 - Organ / stage
- hypocotyl and cotyledon explantse5
- Treatment
- 1 mg l-1 IAA (Indol-3-Acetic Acid) plus 1 mg l-1 2,4-D (2, 4- dichlorophenoxyacetic acid) and 2 mg l-1 kinetine5
- Outcome
- frequency of callus inductione5
- Direction
- higheste5
- Qualifier
- 100 and 150 mM NaCle5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_Parc_Natural_de_s'Albufera%2C_Spain_2022-04-18_(02).jpg?width=500)
Verbatim
t0“In Vitro Selection of Foeniculum vulgare for Salt Tolerance”
e5“in the presence of 100 and 150 mM NaCl, the highest frequency of callus induction in hypocotyl and cotyledon explants was recorded on the media supplemented with 1 mg l-1 IAA (Indol-3-Acetic Acid) plus 1 mg l-1 2,4-D (2, 4- dichlorophenoxyacetic acid) and 2 mg l-1 kinetin”
Claim · model-written summary
The 2 mg/l NAA and 0.5 mg/l kinetin combination was the most effective treatment for shoot regeneration under salt stress.
- Taxon
- Foeniculum vulgare
Anethum foeniculumt0 - Treatment
- 2 mg l-1 NAA (Naphtaleneacetic acid) and 0.5 mg l-1 kinetine4
- Outcome
- shoot regeneratione4
- Direction
- most effectivee4
- Qualifier
- under stress conditione4
- Comparator
- different growth regulator treatmentse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_Parc_Natural_de_s'Albufera%2C_Spain_2022-04-18_(02).jpg?width=500)
Verbatim
t0“In Vitro Selection of Foeniculum vulgare for Salt Tolerance”
e4“Among different growth regulator treatments, the combination of 2 mg l-1 NAA (Naphtaleneacetic acid) and 0.5 mg l-1 kinetin was found to be the most effective for shoot regeneration under stress condition”
The same questions in other papers
sodium chloride · growth · Anethum foeniculum (fennel)Results differ
3 papers in the same direction as this paper · 2 in the opposite direction
- positive
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See all 6 papers →1-naphthaleneacetic acid · growth · Anethum foeniculum (fennel)Results agree
1 paper in the same direction as this paper
- positive
salinity · growth · Anethum foeniculum (fennel)Positive and negative results
8 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.