Claim · model-written summary
External amino acids inhibited glycolysis.
- Taxon
- potato
Solanum tuberosumt0 - Treatment
- External supply of amino acidse0
- Outcome
- glycolysise0
- Direction
- inhibitione0
- Comparator
- sucrosee0
Not reportedMaterialGeneCompoundOrgan / stageInteractorInteractor roleSecond interactorSecond interactor roleSettingValueQualifier
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“External supply of amino acids to replace sucrose as an osmoticum led to a feed‐back inhibition of glycolysis”
t0“potato tubers”
Title
A. R. Fernie; A. Tiessen; M. Stitt; et al. Plant Cell & Environment 2002. doi:10.1046/j.1365-3040.2002.00918.x
Other claims from this paper (17)
- Observation Sucrose phosphorylase activity was less than one tenth of sucrose synthase activity.
- Intervention effect Expression decreased starch synthesis enzyme activities relative to glycolytic enzymes and amylolytic activity.
- Observation Transformants had twice the unidirectional sucrose breakdown rate.
- Observation Sucrose resynthesis was strongly stimulated.
- Observation Net sucrose breakdown changed little.
- Observation Sucrose in the tubers was depleted.
- Observation Glycolysis rates were higher in the transformants.
- Observation Amino acid pools were larger in the transformants.
- Intervention effect Expression decreased tuber ATP/ADP ratio and energy charge.
- Observation At least 30% of respiratory ATP was estimated to be consumed by accelerated sucrose cycling.
- Observation Absolute starch synthesis rose in short-term disc labeling.
- Observation Starch synthesis fell relative to other fluxes.
- Observation Tuber starch content was lower than in wild type.
- Intervention effect External amino acids did not restore allocation to starch.
- Intervention effect Palatinose stimulated flux to starch in transformants.
- Mechanism Lower sucrose and greater energy use during sucrose cycling explain poor tuber performance.
- Mechanism Sucrose synthase degradation helps maintain the sucrose pool and limit ATP consumption.