Abstract
Thioltransferase, also known as glutaredoxin, is an enzyme that catalyzes the reduction of a variety of disulfides, including protein disulfides, in the presence of reduced glutathione. Thioltransferase was purified from kale through ammonium sulfate fractionation, DE-52 ion-exchange chromatography, Sephadex G-75 gel filtration, and Q-Sepharose ion-exchange chromatography. Its molecular size was estimated to be about 31,000 daltons on SDS-PAGE. The purified enzyme has an optimum pH of about 8.0 with 2-hydroxyethyl disulfide as a substrate. The enzyme also utilizes L-sulfocysteine, L-cystine, bovine serum albumin, and insulin as substrates in the presence of GSH. The enzyme has values of 0.24-0.67 mM for these substrates. The enzyme was partly inactivated after heating at or higher temperature for 30 min. The enzyme was stimulated by various thiol compounds such as reduced glutathione, dithiothreitol, L-cysteine, and -mercaptoethanol. This is a second example of a plant thioltransferase which was purified and characterized.
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Claims 10
Claim · model-written summary
Kale thioltransferase was purified.
- Taxon
- kale
Brassica oleracea var. viridise0 - Gene
- Thioltransferasee0
- Outcome
- purifiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thioltransferase was purified from kale”
Claim · model-written summary
Its estimated molecular size was about 31,000 daltons.
- Taxon
- KaleBrassica oleracea var. viridist0
- Outcome
- molecular sizee0
- Value
- about 31,000 daltonse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Its molecular size was estimated to be about 31,000 daltons on SDS-PAGE”
t0“Kale”
Claim · model-written summary
The enzyme had an optimum pH of about 8.0 with 2-hydroxyethyl disulfide.
- Taxon
- KaleBrassica oleracea var. viridist0
- Outcome
- optimum pHe0
- Value
- about 8.0e0
- Qualifier
- 2-hydroxyethyl disulfide as a substratee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The purified enzyme has an optimum pH of about 8.0 with 2-hydroxyethyl disulfide as a substrate”
t0“Kale”
Claim · model-written summary
The enzyme uses L-sulfocysteine, L-cystine, bovine serum albumin, and insulin as substrates in the presence of GSH.
- Taxon
- KaleBrassica oleracea var. viridist0
- Outcome
- utilizes L-sulfocysteine, L-cystine, bovine serum albumin, and insulin as substratese0
- Qualifier
- in the presence of GSHe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme also utilizes L-sulfocysteine, L-cystine, bovine serum albumin, and insulin as substrates in the presence of GSH”
t0“Kale”
Claim · model-written summary
Values for these substrates were 0.24-0.67 mM.
- Taxon
- KaleBrassica oleracea var. viridist0
- Outcome
- valuese0
- Value
- 0.24-0.67 mMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme has values of 0.24-0.67 mM for these substrates”
t0“Kale”
Claim · model-written summary
Heating for 30 minutes partly inactivated the enzyme.
- Taxon
- KaleBrassica oleracea var. viridist0
- Treatment
- heating at or higher temperature for 30 mine0
- Outcome
- enzymee0
- Direction
- partly inactivatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme was partly inactivated after heating at or higher temperature for 30 min”
t0“Kale”
Claim · model-written summary
Reduced glutathione stimulated the enzyme.
- Taxon
- KaleBrassica oleracea var. viridist0
- Treatment
- reduced glutathionee0
- Outcome
- enzymee0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme was stimulated by various thiol compounds such as reduced glutathione, dithiothreitol, L-cysteine, and -mercaptoethanol”
t0“Kale”
Claim · model-written summary
Dithiothreitol stimulated the enzyme.
- Taxon
- KaleBrassica oleracea var. viridist0
- Treatment
- dithiothreitole0
- Outcome
- enzymee0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme was stimulated by various thiol compounds such as reduced glutathione, dithiothreitol, L-cysteine, and -mercaptoethanol”
t0“Kale”
Claim · model-written summary
L-cysteine stimulated the enzyme.
- Taxon
- KaleBrassica oleracea var. viridist0
- Treatment
- L-cysteinee0
- Outcome
- enzymee0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme was stimulated by various thiol compounds such as reduced glutathione, dithiothreitol, L-cysteine, and -mercaptoethanol”
t0“Kale”
Claim · model-written summary
-mercaptoethanol stimulated the enzyme.
- Taxon
- KaleBrassica oleracea var. viridist0
- Treatment
- -mercaptoethanole0
- Outcome
- enzymee0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The enzyme was stimulated by various thiol compounds such as reduced glutathione, dithiothreitol, L-cysteine, and -mercaptoethanol”
t0“Kale”
The same questions in other papers
heat · physiology: enzyme activity · Brassica oleracea (wild cabbage)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.