Abstract
Chlorophyll (Chl) fluorescence quenching parameters were measured in dark-adapted pea leaves and chloroplasts with the purpose to find the conditions of high and low non-photochemical quenching, that would be stable during a prolonged irradiation. A PAM fluorometer was used for measuring induction curves in the range of actinic radiation of 3-35 W m-2, with an ordinary value of about 15 W m-2. The effects of various mediators, i.e., ascorbate, methyl viologen (MV), dithiothreitol (DTT) and nigericin, on the quenching process were tested. Simultaneously, the absorbance was measured during a 15-20 min period of irradiation and after the actinic radiation was turned off, i.e., in the recovery period. The pH values of chloroplast suspensions were 5.5, 6.5 and 8.0, the largest non-photochemical quenching was observed at pH of 6.5. The irradiation of chloroplasts led to an absorption decrease within the entire photosynthetically active range, attaining saturation when the fluorescence reached Fs level, and to an absorption increase during the recovery period. Absorbance changes at the maximum of red band were 10-20 %. A decrease in Chl concentration (10 %) after irradiation was found only at pH of 5.5, when the recovery time was the longest, i.e., about 60 min.
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Claims 5
Claim · model-written summary
Non-photochemical quenching was largest at pH 6.5.
- Taxon
- pea
Lathyrus oleraceust0 - Organ / stage
- chloroplastst0
- Outcome
- non-photochemical quenchinge0
- Direction
- largeste0
- Setting
- pH of 6.5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pea chloroplasts”
e0“The pH values of chloroplast suspensions were 5.5, 6.5 and 8.0, the largest non-photochemical quenching was observed at pH of 6.5.”
Claim · model-written summary
Irradiation decreased chloroplast absorption across the photosynthetically active range.
- Taxon
- peaLathyrus oleraceust0
- Organ / stage
- chloroplastst0
- Treatment
- irradiatione0
- Outcome
- absorptione0
- Direction
- decreasee0
- Qualifier
- entire photosynthetically active rangee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pea chloroplasts”
e0“The irradiation of chloroplasts led to an absorption decrease within the entire photosynthetically active range, attaining saturation when the fluorescence reached Fs level, and to an absorption increase during the recovery period.”
Claim · model-written summary
Absorption increased during the recovery period.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- absorptione0
- Direction
- increasee0
- Qualifier
- recovery periode0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pea chloroplasts”
e0“to an absorption increase during the recovery period.”
Claim · model-written summary
Red-band absorbance changes were 10–20%.
- Taxon
- peaLathyrus oleraceust0
- Outcome
- Absorbance changese0
- Value
- 10-20 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pea chloroplasts”
e0“Absorbance changes at the maximum of red band were 10-20 %.”
Claim · model-written summary
Chlorophyll concentration decreased 10% after irradiation at pH 5.5, where recovery took about 60 minutes.
- Taxon
- peaLathyrus oleraceust0
- Treatment
- irradiatione0
- Outcome
- Chl concentratione0
- Direction
- decreasee0
- Value
- 10 %e0
- Qualifier
- about 60 mine0
- Setting
- pH of 5.5e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“pea chloroplasts”
e0“A decrease in Chl concentration (10 %) after irradiation was found only at pH of 5.5, when the recovery time was the longest, i.e., about 60 min.”
The same questions in other papers
radiation · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
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See all 9 papers →radiation · nutrient: content · Lathyrus oleraceus (pea)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.