Abstract
In radicle meristem cells of germinating seeds of the pea (Pisum sativum L) before the onset of replicative synthesis of DNA, irradiation with 2-3 krad of gamma-rays induced the incorporation of 3H-thymidine (3H-TdR). Maximum isotope incorporation was noted during the first 2 hours after irradiation. Higher doses of radiation suppressed 3H-TdR incorporation. It was not seen after gamma-irradiation of air-dried seeds, nor after fast-neutron irradiation. The replication inhibitors hydroxyurea and 5-aminouracil had no effect on the gamma-induced incorporation of 3H-TdR, Whereas caffeine and acriflavine inhibited it to some extent. It is suggested that the gamma-radiation-induced incorporation of 3H-TdR in meristem cells during the pre-replicative period may be connected with repair phenomena.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Gamma irradiation at 2-3 krad induced 3H-thymidine incorporation in pea radicle meristem cells.
- Taxon
- pea (Pisum sativum L)
Lathyrus oleraceuse0 - Organ / stage
- radicle meristem cells of germinating seedse0
- Treatment
- irradiation with 2-3 krad of gamma-rayse0
- Outcome
- incorporation of 3H-thymidine (3H-TdR)e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In radicle meristem cells of germinating seeds of the pea (Pisum sativum L) before the onset of replicative synthesis of DNA, irradiation with 2-3 krad of gamma-rays induced the incorporation of 3H-thymidine (3H-TdR).”
Claim · model-written summary
Incorporation peaked in the first two hours after irradiation.
Verbatim
e0“Maximum isotope incorporation was noted during the first 2 hours after irradiation.”
t0“Pisum Sativum L.”
Claim · model-written summary
Higher radiation doses suppressed 3H-TdR incorporation.
- Taxon
- Pisum Sativum L.t0
- Treatment
- Higher doses of radiatione0
- Outcome
- 3H-TdR incorporatione0
- Direction
- suppressede0
Not reportedComparator
Verbatim
e0“Higher doses of radiation suppressed 3H-TdR incorporation.”
t0“Pisum Sativum L.”
Claim · model-written summary
Gamma irradiation of air-dried seeds did not induce 3H-TdR incorporation.
- Taxon
- Pisum Sativum L.t0
- Organ / stage
- air-dried seedse0
- Treatment
- gamma-irradiation of air-dried seedse0
- Outcome
- incorporation of 3H-thymidine (3H-TdR)e1
- Direction
- not seene0
Not reportedComparator
Verbatim
e0“It was not seen after gamma-irradiation of air-dried seeds, nor after fast-neutron irradiation.”
e1“irradiation with 2-3 krad of gamma-rays induced the incorporation of 3H-thymidine (3H-TdR)”
t0“Pisum Sativum L.”
Claim · model-written summary
Fast-neutron irradiation did not induce the incorporation.
- Taxon
- Pisum Sativum L.t0
- Treatment
- fast-neutron irradiatione0
- Outcome
- incorporation of 3H-thymidine (3H-TdR)e1
- Direction
- not seene0
Not reportedComparator
Verbatim
e0“It was not seen after gamma-irradiation of air-dried seeds, nor after fast-neutron irradiation.”
e1“irradiation with 2-3 krad of gamma-rays induced the incorporation of 3H-thymidine (3H-TdR)”
t0“Pisum Sativum L.”
Claim · model-written summary
Hydroxyurea had no effect on gamma-induced incorporation.
- Taxon
- Pisum Sativum L.t0
- Treatment
- hydroxyureae0
- Outcome
- gamma-induced incorporation of 3H-TdRe0
- Direction
- no effecte0
Not reportedComparator
Verbatim
e0“The replication inhibitors hydroxyurea and 5-aminouracil had no effect on the gamma-induced incorporation of 3H-TdR, Whereas caffeine and acriflavine inhibited it to some extent.”
t0“Pisum Sativum L.”
Claim · model-written summary
5-aminouracil had no effect on gamma-induced incorporation.
- Taxon
- Pisum Sativum L.t0
- Treatment
- 5-aminouracile0
- Outcome
- gamma-induced incorporation of 3H-TdRe0
- Direction
- no effecte0
Not reportedComparator
Verbatim
e0“The replication inhibitors hydroxyurea and 5-aminouracil had no effect on the gamma-induced incorporation of 3H-TdR, Whereas caffeine and acriflavine inhibited it to some extent.”
t0“Pisum Sativum L.”
Claim · model-written summary
Caffeine inhibited gamma-induced incorporation to some extent.
- Taxon
- Pisum Sativum L.t0
- Treatment
- caffeinee0
- Outcome
- gamma-induced incorporation of 3H-TdRe0
- Direction
- inhibitede0
- Qualifier
- to some extente0
Not reportedComparator
Verbatim
e0“The replication inhibitors hydroxyurea and 5-aminouracil had no effect on the gamma-induced incorporation of 3H-TdR, Whereas caffeine and acriflavine inhibited it to some extent.”
t0“Pisum Sativum L.”
Claim · model-written summary
Acriflavine inhibited gamma-induced incorporation to some extent.
- Taxon
- Pisum Sativum L.t0
- Treatment
- acriflavinee0
- Outcome
- gamma-induced incorporation of 3H-TdRe0
- Direction
- inhibitede0
- Qualifier
- to some extente0
Not reportedComparator
Verbatim
e0“The replication inhibitors hydroxyurea and 5-aminouracil had no effect on the gamma-induced incorporation of 3H-TdR, Whereas caffeine and acriflavine inhibited it to some extent.”
t0“Pisum Sativum L.”
Claim · model-written summary
Gamma-induced incorporation may be connected with DNA repair.
Verbatim
e0“It is suggested that the gamma-radiation-induced incorporation of 3H-TdR in meristem cells during the pre-replicative period may be connected with repair phenomena.”
t0“Pisum Sativum L.”
The same questions in other papers
radiation · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
- no effect
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.