Abstract
Adenine nucleotide translocase (ANT) is a constitutive mitochondrial component that is involved in ADP/ATP exchange and mitochondrion-mediated apoptosis in yeast and mammals. However, little is known about the function of ANT in pathogenic fungi. In this study, we identified an ANT gene of Puccinia striiformis f. sp. tritici (Pst), designated PsANT. The PsANT protein contains three typical conserved mitochondrion-carrier-protein (mito-carr) domains and shares more than 70% identity with its orthologs from other fungi, suggesting that ANT is conserved in fungi. Immuno-cytochemical localization confirmed the mitochondrial localization of PsANT in normal Pst hyphal cells or collapsed cells. Over-expression of PsANT indicated that PsANT promotes cell death in tobacco, wheat and fission yeast cells. Further study showed that the three mito-carr domains are all needed to induce cell death. qRT-PCR analyses revealed an in-planta induced expression of PsANT during infection. Knockdown of PsANT using a host-induced gene silencing system (HIGS) attenuated the growth and development of virulent Pst at the early infection stage but not enough to alter its pathogenicity. These results provide new insight into the function of PsANT in fungal cell death and growth and might be useful in the search for and design of novel disease control strategies.
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Claims 8
Claim · model-written summary
The PsANT protein has three conserved mitochondrial carrier domains and over 70% identity with fungal orthologs.
- Taxon
- tobacco
Nicotiana tabacume1 - Gene
- PsANTe0
- Outcome
- three typical conserved mitochondrion-carrier-protein (mito-carr) domains and shares more than 70% identitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The PsANT protein contains three typical conserved mitochondrion-carrier-protein (mito-carr) domains and shares more than 70% identity with its orthologs from other fungi”
e1“cell death in tobacco, wheat and fission yeast cells”
Claim · model-written summary
PsANT localized to mitochondria of normal or collapsed Pst hyphal cells.
- Taxon
- wheat
Triticum aestivume1 - Organ / stage
- hyphal cellse0
- Gene
- PsANTe0
- Outcome
- mitochondrial localization of PsANTe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immuno-cytochemical localization confirmed the mitochondrial localization of PsANT in normal Pst hyphal cells or collapsed cells.”
e1“cell death in tobacco, wheat and fission yeast cells”
Claim · model-written summary
PsANT overexpression promoted cell death in tobacco cells.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- Over-expression of PsANTe0
- Gene
- PsANTe0
- Outcome
- cell deathe0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Over-expression of PsANT indicated that PsANT promotes cell death in tobacco, wheat and fission yeast cells.”
Claim · model-written summary
PsANT overexpression promoted cell death in wheat cells.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Over-expression of PsANTe0
- Gene
- PsANTe0
- Outcome
- cell deathe0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Over-expression of PsANT indicated that PsANT promotes cell death in tobacco, wheat and fission yeast cells.”
Claim · model-written summary
All three mitochondrial carrier domains were needed for PsANT-induced cell death.
- Taxon
- wheatTriticum aestivume1
- Gene
- mito-carr domainse0
- Outcome
- cell deathe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the three mito-carr domains are all needed to induce cell death”
e1“cell death in tobacco, wheat and fission yeast cells”
Claim · model-written summary
PsANT expression was induced in planta during infection.
- Taxon
- wheatTriticum aestivume1
- Gene
- PsANTe0
- Outcome
- expression of PsANTe0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“qRT-PCR analyses revealed an in-planta induced expression of PsANT during infection.”
e1“cell death in tobacco, wheat and fission yeast cells”
Claim · model-written summary
Host-induced silencing of PsANT attenuated virulent Pst growth and development early in infection.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Knockdown of PsANTe0
- Interactor
- Pste0pathogen
- Gene
- PsANTe0
- Outcome
- growth and development of virulent Pste0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockdown of PsANT using a host-induced gene silencing system (HIGS) attenuated the growth and development of virulent Pst at the early infection stage but not enough to alter its pathogenicity.”
e1“cell death in tobacco, wheat and fission yeast cells”
Claim · model-written summary
Host-induced PsANT silencing did not alter Pst pathogenicity.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Knockdown of PsANTe0
- Interactor
- Pste0pathogen
- Gene
- PsANTe0
- Outcome
- its pathogenicitye0
- Direction
- not enough to altere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockdown of PsANT using a host-induced gene silencing system (HIGS) attenuated the growth and development of virulent Pst at the early infection stage but not enough to alter its pathogenicity.”
e1“cell death in tobacco, wheat and fission yeast cells”
The same questions in other papers
loss of function · disease · Triticum aestivum (wheat)Positive and negative results
80 papers in the same direction as this paper · 96 in the opposite direction
- no effect
- negative
- negative
173 papers more in this group
See all 177 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.